Supports multicolor fluorescent studies for imaging of living, whole mount or thickly sliced specimens. Dynamic biological processes can be imaged hundreds of micrometers within living cells and tissues. Provides support for applications where phototoxicity/photobleaching are a concern such as time course studies of living cells and tissues. Low magnification lens and long working distance stage allow imaging of large samples, embryos, and animals. A range of microprobe objectives are available for minimally invasive in vivo imaging of deep tissues. This microscope also offers conventional confocal laser scanning of samples on slide.
|Laser source||Excitation wavelength||Dyes & Fluorophores||Emission color|
|405 Diode||405 nm||DAPI, Hoechst||Violet|
|Multi-line Argon||457 nm||CFP||Cyan|
|488 nm||Alexa 488, Oregon Green, FITC, GFP, EGFP, DiO, Cy2||Green|
|514 nm||YFP, EYFP||Yellow|
|Green HeNe||543 nm||Cy3, TRITC, mCherry, Alexa 543, Alexa 594||Orange-red|
|Red HeNe||633 nm||Alexa 633, Alexa 647, Cy5, TO-PRO3,||Far red|
Fluorochrome excitation with multi-photon IR laser.
|Emission color||Dye||Excitation (FV1000-tested)|
|Blue/cyan||Alexa 350||780-800 nm|
|Hoechst||780-800 nm, 900-1100 nm|
|DAPI||780-800 nm, 900-1100 nm|
|Green||Oregon Green||800-860 nm|
|Alexa 488||800-830 nm, (860 nm, 910-920 nm)|
|FITC||750-800 nm, (860 nm, 910-920 nm)|
|Yellow / Orange||YFP||890-950 nm|
|Rhodamine B||800-860 nm|
|Alexa 568||780-840 nm|
Download and view Olympus FV1000 User Guide.
For viewing and basic image handling, a free viewer software (FV10-ASW viewer) is available.
For more information on FV1000MPE system, click on http://microscope.olympus-global.com/en/ga/product/fv1000mpe/index.cfm
Multiphoton vs. Single-photon laser scanning.
One of the main advantage of multiphoton laser scanning microscopy comes from the use of infra-red (IR) lasers and signal detection without pinhole. IR lasers seem to penetrate more deeply than shorter-wavelength visible lasers and, with no need for pinhole, more light signals are collected, consequently increasing the resolution and contrast of image from deep and thick sample. This enhancement is well demonstrated by the figure below, in which a Drosophila embryo (~200 µm thick) stained with FITC-conjugated anti-a-tubulin antibody was imaged with 488nm visible laser (single photon) or 800nm IR laser (multiphoton) on Olympus FV1000 confocal microscope.
Multiphoton laser scanning image.
3T3 cells are stained with DAPI and anti-tubulin (green) antibody and imaged with 800 nm to 920 nm laser to visualize both DAPI and green Alexa fluoro 488 antibody. As seen in the figures, DAPI and Alexa 488 exhibit different excitation and emission profiles over various wavelength; DAPI is excited well with 820~840 nm laser, whereas Alexa 488 shows better exciation with 880~920 nm laser. It is noticeable that some DAPI emissions appear to be detected in Alexa 488 (green) channel at shorter wavelength (i.e. 800 nm), about which one needs to be careful during double immunofluorescence imaging.