USF Genomics

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The USF Genomics Program has the capability to assist with both sample sequencing and computational analysis for research projects at USF.


  • USF Genomics Core

    The USF Genomics Equipment Core is a NextGen Sequencing laboratory facility which consolidates resources and expertise to provide genomics laboratory training from the Library preparation to Next Generation Sequencing. The core offers assistance with experimental design, trouble shooting, and data analysis. Certified users may reserve time in the USF Genomics Equipment Core for library prep and sequencing. Completion of the USF Genomics RNAseq Laboratory Training is required to become a certified user of the Core. We welcome members of the USF community to contact us regarding the following services:

     

    Equipment for NextGen Sequencing:

    • Illumina - NextSeq-500
    • Illumina - MiSeq System
    • 10X GENOMICS- Single Cell Instrument - Chromium Controller

    Instrumentation for library-prep and quality-assurance:

    • Agilent - TapeStation 2200, TapeStation 4200 (DNA and RNA quality assessment)
    • Invitrogen Qubit 2.0 fluorometer (DNA and RNA sample quantification)
    • Roche - LightCycler 96 Real-time PCR Instrument (DNA library quantification)
    • Covaris - M220 Focused Sonicator (DNA fragmentation)

     

    More Information

    USF Genomics Core Policies and Procedures

    USF Genomics Core Service Agreement

    Sample Submission Sheet

    USF Genomics Core Rate Sheet

    RNAseq Protocols

     

    Certified Users can submit work requests to the USF Genomics Core laboratory facilities through iLab: https://my.ilabsolutions.com/service_center/show_external/5175      

     

    Questions regarding the USF Genomics Equipment Core can be sent to genomics@health.usf.edu.

  • USF Genomics Hub

    The USF Genomics Hub is the computational branch of the USF Genomics Core. The Hub consists of computational biology consultants for genomics research projects. These consultants run Mac, Linux and Windows workstations and have access to USF’s High-Performance Computing (HPC) cluster and a variety of both proprietary and open-source, command-line based analysis software. Data analyzed by the Hub are securely stored on a new HIPAA-compliant component of USF’s HPC cluster. HPC infrastructure and hardware supporting USF Genomics and the USF Genomics Core Facility is managed by the USF Department of Research Computing.

    The members of the USF Genomics Hub also are instructors for the USF Genomics RNAseq Data Analysis Training. This training reviews theoretical and practical concepts related to RNA sequencing analysis, enabling them to perform these analyses independently.

    To place requests for computational assistance from the USF Genomics Program, please submit queries through the link below. After submitting a request, a member of the Hub will reach out to set up a consultation time.

    USF Genomics Computational Inquiry Form

    For more information, contact genomics@health.usf.edu.

  • Grant Resources

    The USF Genomics Program has assembled resources for PI's at USF to use when applying for grants. The following document include the resources and facilities available through the USF Genomics Program:

    USF Genomics Facilities and Resources