Samuel Wickline, MD

Tgh Endowed Chair, Cardiovascular Medicine

Associate Dean, College of Medicine Cardiology

Professor, Cardiovascular Sciences

Director, USF Health Heart Institute

Contact Info 560 Channelside Drive
Tampa FL 33602

Academic Email: wickline@usf.edu

Education

Interdisciplinary and Emerging Signature Programs

• Biomedical Engineering & Nanomedicine
• Cardiovascular Sciences

Research Interests

• My laboratory pursues basic and clinical research topics in molecular imaging and nanotechnology. We design novel methods, hardware, and software for clinical and molecular imaging with ultrasound and MRI. Cross disciplinary nanomedicine research is conducted with many collaborators. Current areas of research interest include:
• 1. Development of molecularly targeted peptide nanostructures for control of gene function by delivery of oligonucleotides and mRNA to modulate inflammatory diseases.
• 2. The design of theranostic biocompatible perfluorocarbon nanostructures for quantitative magnetic resonance imaging and delivery of pharmaceutical agents in atherosclerosis and heart failure.
• 3. The design and synthesis of protease-activatible nanostructures for therapeutic use in metastatic cancer and atherosclerosis.
• 4. The development of nanostructures that restore cellular autophagy in diseases such as muscular dystrophy and heart failure.

Recent Publications

• Zou W, Rohatgi N, Brestoff JR, Moley JR, Li Y, Williams JW, Alippe Y, Pan H, Pietka TA, Mbalaviele G, Newberry EP, Davidson NO, Dey A, Shoghi KI, Head RD, Wickline SA, Randolph GJ, Abumrad NA, Teitelbaum SL. Myeloid-specific Asxl2 deletion limits diet-induced obesity by regulating energy expenditure. The Journal of clinical investigation. , 2020.
• Yan H, Hu Y, Akk A, Rai MF, Pan H, Wickline SA, Pham CTN. Induction of WNT16 via Peptide-mRNA Nanoparticle-Based Delivery Maintains Cartilage Homeostasis. Pharmaceutics. 12(1) , 2020.
• Wilson AJ, Zhou Q, Vargas I, Palekar R, Grabau R, Pan H, Wickline SA. Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles. Methods in molecular biology (Clifton, N.J.). 2118: 111-120, 2020.
• Rai MF, Pan H, Yan H, Sandell LJ, Pham CTN, Wickline SA. Applications of RNA interference in the treatment of arthritis. Translational research : the journal of laboratory and clinical medicine. 214: 1-16, 2019.
• Marsh JN, Korenblat KM, Liu TC, McCarthy JE, Wickline SA. Resolution of Murine Toxic Hepatic Injury Quantified With Ultrasound Entropy Metrics. Ultrasound in medicine & biology. 45(10) : 2777-2786, 2019.
• MohanKumar K, Namachivayam K, Song T, Jake Cha B, Slate A, Hendrickson JE, Pan H, Wickline SA, Oh JY, Patel RP, He L, Torres BA, Maheshwari A. A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions. Nature communications. 10(1) : 3494, 2019.
• Hamilton AR, Traniello IM, Ray AM, Caldwell AS, Wickline SA, Robinson GE. Division of labor in honey bees is associated with transcriptional regulatory plasticity in the brain. The Journal of experimental biology. 222(Pt 14) , 2019.
• Strand MS, Krasnick BA, Pan H, Zhang X, Bi Y, Brooks C, Wetzel C, Sankpal N, Fleming T, Goedegebuure SP, DeNardo DG, Gillanders WE, Hawkins WG, Wickline SA, Fields RC. Precision delivery of RAS-inhibiting siRNA to KRAS driven cancer via peptide-based nanoparticles. Oncotarget. 10(46) : 4761-4775, 2019.
• Mills KA, Quinn JM, Roach ST, Palisoul M, Nguyen M, Noia H, Guo L, Fazal J, Mutch DG, Wickline SA, Pan H, Fuh KC. p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts. Scientific reports. 9(1) : 4762, 2019.
• Yan H, Duan X, Pan H, Akk A, Sandell LJ, Wickline SA, Rai MF, Pham CTN. Development of a peptide-siRNA nanocomplex targeting NF- κB for efficient cartilage delivery. Scientific reports. 9(1) : 442, 2019.
• McGill JB, Johnson M, Hurst S, Cade WT, Yarasheski KE, Ostlund RE, Schechtman KB, Razani B, Kastan MB, McClain DA, de Las Fuentes L, Davila-Roman VG, Ory DS, Wickline SA, Semenkovich CF. Low dose chloroquine decreases insulin resistance in human metabolic syndrome but does not reduce carotid intima-media thickness. Diabetology & metabolic syndrome. 11: 61, 2019.
• Yan H, Duan X, Collins KH, Springer LE, Guilak F, Wickline SA, Rai MF, Pan H, Pham CTN. Nanotherapy Targeting NF-κB Attenuates Acute Pain After Joint Injury. Precision nanomedicine. 2(1) : 245-248, 2019.
• Vemuri C, Upadhya GA, Arif B, Jia J, Lin Y, Gaut JP, Fazal J, Pan H, Wickline SA, Chapman WC. Antithrombin Perfluorocarbon Nanoparticles Improve Renal Allograft Function in a Murine Deceased Criteria Donor Model. Transplantation direct. 4(9) : e384, 2018.
• Moore JK, Chen J, Pan H, Gaut JP, Jain S, Wickline SA. Quantification of vascular damage in acute kidney injury with fluorine magnetic resonance imaging and spectroscopy. Magnetic resonance in medicine. 79(6) : 3144-3153, 2018.
• Kabir AU, Lee TJ, Pan H, Berry JC, Krchma K, Wu J, Liu F, Kang HK, Hinman K, Yang L, Hamilton S, Zhou Q, Veis DJ, Mecham RP, Wickline SA, Miller MJ, Choi K. Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis. JCI insight. 3(8) , 2018.
• Pan H, Palekar RU, Hou KK, Bacon J, Yan H, Springer LE, Akk A, Yang L, Miller MJ, Pham CT, Schlesinger PH, Wickline SA. Anti-JNK2 peptide-siRNA nanostructures improve plaque endothelium and reduce thrombotic risk in atherosclerotic mice. International journal of nanomedicine. 13: 5187-5205, 2018.
• Vendrov AE, Stevenson MD, Alahari S, Pan H, Wickline SA, Madamanchi NR, Runge MS. Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice. Journal of the American Heart Association. 6(11) , 2017.
• Thairu MW, Skidmore IH, Bansal R, Nováková E, Hansen TE, Li-Byarlay H, Wickline SA, Hansen AK. Efficacy of RNA interference knockdown using aerosolized short interfering RNAs bound to nanoparticles in three diverse aphid species. Insect Molecular Biology. 26(3) : 356-368, 2017.
• Lozhkin A, Vendrov AE, Pan H, Wickline SA, Madamanchi NR, Runge MS. NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis. Journal of Molecular and Cellular Cardiology. 102: 10-21, 2017.
• Pan D, Pham CT, Weilbaecher KN, Tomasson MH, Wickline SA, Lanza GM. Contact-facilitated drug delivery with Sn2 lipase labile prodrugs optimize targeted lipid nanoparticle drug delivery. Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology. 8(1) : 85-106, 2016.
• Palekar RU, Vemuri C, Marsh JN, Arif B, Wickline SA. Antithrombin nanoparticles inhibit stent thrombosis in ex vivo static and flow models. Journal of Vascular Surgery. 64(5) : 1459-1467, 2016.
• Yan H, Duan X, Pan H, Holguin N, Rai MF, Akk A, Springer LE, Wickline SA, Sandell LJ, Pham CT. Suppression of NF-κB activity via nanoparticle-based siRNA delivery alters early cartilage responses to injury. Proceedings of the National Academy of Sciences of the United States of America. 113(41) : E6199-E6208, 2016.
• Palekar RU, Jallouk AP, Myerson JW, Pan H, Wickline SA. Inhibition of Thrombin With PPACK-Nanoparticles Restores Disrupted Endothelial Barriers and Attenuates Thrombotic Risk in Experimental Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 36(3) : 446-55, 2016.
• Esser AK, Schmieder AH, Ross MH, Xiang J, Su X, Cui G, Zhang H, Yang X, Allen JS, Williams T, Wickline SA, Pan D, Lanza GM, Weilbaecher KN. Dual-therapy with αvβ3-targeted Sn2 lipase-labile fumagillin-prodrug nanoparticles and zoledronic acid in the Vx2 rabbit tumor model. Nanomedicine : Nanotechnology, Biology, and Medicine. 12(1) : 201-11, 2016.
• Schmieder AH, Caruthers SD, Keupp J, Wickline SA, Lanza GM. Recent Advances in (19)Fluorine Magnetic Resonance Imaging with Perfluorocarbon Emulsions. Engineering (Beijing, China). 1(4) : 475-489, 2015.
• Hou KK, Pan H, Schlesinger PH, Wickline SA. A role for peptides in overcoming endosomal entrapment in siRNA delivery - A focus on melittin. Biotechnology advances. 33(6 Pt 1) : 931-40, 2015.
• Goette MJ, Keupp J, Rahmer J, Lanza GM, Wickline SA, Caruthers SD. Balanced UTE-SSFP for 19F MR imaging of complex spectra. Magnetic Resonance in Medicine. 74(2) : 537-43, 2015.
• Goette MJ, Lanza GM, Caruthers SD, Wickline SA. Improved quantitative (19) F MR molecular imaging with flip angle calibration and B1 -mapping compensation. Journal of Magnetic Resonance Imaging : JMRI. 42(2) : 488-94, 2015.
• Jallouk AP, Palekar RU, Marsh JN, Pan H, Pham CT, Schlesinger PH, Wickline SA. Delivery of a Protease-Activated Cytolytic Peptide Prodrug by Perfluorocarbon Nanoparticles. Bioconjugate Chemistry. 26(8) : 1640-50, 2015.
• Palekar RU, Jallouk AP, Goette MJ, Chen J, Myerson JW, Allen JS, Akk A, Yang L, Tu Y, Miller MJ, Pham CT, Wickline SA, Pan H. Quantifying progression and regression of thrombotic risk in experimental atherosclerosis. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. 29(7) : 3100-9, 2015.
• Pham CT, Pan H, Wickline SA. Peptide-siRNA nanotherapeutics in arthritis. Oncotarget. 6(17) : 14731-2, 2015.
• Palekar RU, Jallouk AP, Lanza GM, Pan H, Wickline SA. Molecular imaging of atherosclerosis with nanoparticle-based fluorinated MRI contrast agents. Nanomedicine (London, England). 10(11) : 1817-32, 2015.
• Hughes MS, McCarthy JE, Bruillard PJ, Marsh JN, Wickline SA. Entropy vs. Energy Waveform Processing: A Comparison Based on the Heat Equation. Entropy (Basel, Switzerland). 17(6) : 3518-3551, 2015.
• Pham CT, Pan H, Wickline SA. Peptide-siRNA nanotherapeutics in arthritis. Oncotarget. : 14731-14732, 2015.
• Chen J, Vemuri C, Palekar RU, Gaut JP, Goette M, Hu L, Cui G, Zhang H, Wickline SA. Antithrombin nanoparticles improve kidney reperfusion and protect kidney function after ischemia-reperfusion injury. American journal of physiology. Renal physiology. 308(7) : F765-73, 2015.
• Jallouk AP, Palekar RU, Pan H, Schlesinger PH, Wickline SA. Modifications of natural peptides for nanoparticle and drug design. Advances in Protein Chemistry and Structural Biology. 98: 57-91, 2015.
• Wang K, Pan D, Schmieder AH, Senpan A, Hourcade DE, Pham CT, Mitchell LM, Caruthers SD, Cui G, Wickline SA, Shen B, Lanza GM. Synergy between surface and core entrapped metals in a mixed manganese-gadolinium nanocolloid affords safer MR imaging of sparse biomarkers. Nanomedicine : Nanotechnology, Biology, and Medicine. 11(3) : 601-9, 2015.
• Hughes MS, Marsh JN, Wickline SA, McCarthy JE. Additional results for "joint entropy of continuously differentiable ultrasonic waveforms" [J. Acoust. Soc. Am. 133(1), 283-300 (2013)]. The Journal of the Acoustical Society of America. 137(1) : 501, 2015.
• Pan D, Kim B, Hu G, Gupta DS, Senpan A, Yang X, Schmieder A, Swain C, Wickline SA, Tomasson MH, Lanza GM. A strategy for combating melanoma with oncogenic c-Myc inhibitors and targeted nanotherapy. Nanomedicine (London, England). 10(2) : 241-51, 2015.
• Myerson JW, He L, Allen JS, Williams T, Lanza G, Tollefsen D, Caruthers S, Wickline S. Thrombin-inhibiting nanoparticles rapidly constitute versatile and detectable anticlotting surfaces. Nanotechnology. 25(39) : 395101, 2014.
• Bulte JW, Schmieder AH, Keupp J, Caruthers SD, Wickline SA, Lanza GM. MR cholangiography demonstrates unsuspected rapid biliary clearance of nanoparticles in rodents: implications for clinical translation. Nanomedicine : nanotechnology, biology, and medicine. 10(7) : 1385-8, 2014.
• Hu L, Wickline SA, Hood JL. Magnetic resonance imaging of melanoma exosomes in lymph nodes. Magnetic Resonance in Medicine. , 2014.
• Zhou HF, Yan H, Hu Y, Springer LE, Yang X, Wickline SA, Pan D, Lanza GM, Pham CT. Fumagillin prodrug nanotherapy suppresses macrophage inflammatory response via endothelial nitric oxide. ACS Nano. 8(7) : 7305-17, 2014.
• Bibee KP, Cheng YJ, Ching JK, Marsh JN, Li AJ, Keeling RM, Connolly AM, Golumbek PT, Myerson JW, Hu G, Chen J, Shannon WD, Lanza GM, Weihl CC, Wickline SA. Rapamycin nanoparticles target defective autophagy in muscular dystrophy to enhance both strength and cardiac function. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. 28(5) : 2047-61, 2014.
• Jamis-Dow CA, Barbier GH, Watkins MP, Lanza GM, Caruthers SD, Wickline SA. Bicuspid Pulmonic Valve and Pulmonary Artery Aneurysm. Cardiology Research. 5(2) : 83-84, 2014.
• Pan D, Schmieder AH, Wang K, Yang X, Senpan A, Cui G, Killgore K, Kim B, Allen JS, Zhang H, Caruthers SD, Shen B, Wickline SA, Lanza GM. Anti-angiogenesis therapy in the Vx2 rabbit cancer model with a lipase-cleavable Sn 2 taxane phospholipid prodrug using α(v)β₃-targeted theranostic nanoparticles. Theranostics. 4(6) : 565-78, 2014.
• Jallouk AP, Moley KH, Omurtag K, Hu G, Lanza GM, Wickline SA, Hood JL. Nanoparticle incorporation of melittin reduces sperm and vaginal epithelium cytotoxicity. PloS One. 9(4) : e95411, 2014.
• Schmieder AH, Wang K, Zhang H, Senpan A, Pan D, Keupp J, Caruthers SD, Wickline SA, Shen B, Wagner EM, Lanza GM. Characterization of early neovascular response to acute lung ischemia using simultaneous (19)F/ (1)H MR molecular imaging. Angiogenesis. 17(1) : 51-60, 2014.
• Palekar RU, Myerson JW, Schlesinger PH, Sadler JE, Pan H, Wickline SA. Thrombin-targeted liposomes establish a sustained localized anticlotting barrier against acute thrombosis. Molecular pharmaceutics. 10(11) : 4168-75, 2013.
• Chen J, Pan H, Lanza GM, Wickline SA. Perfluorocarbon nanoparticles for physiological and molecular imaging and therapy. Advances in chronic kidney disease. 20(6) : 466-78, 2013.
• Schmieder AH, Winter PM, Williams TA, Allen JS, Hu G, Zhang H, Caruthers SD, Wickline SA, Lanza GM. Molecular MR imaging of neovascular progression in the Vx2 tumor with αvβ3-targeted paramagnetic nanoparticles. Radiology. 268(2) : 470-80, 2013.
• Hou KK, Pan H, Lanza GM, Wickline SA. Melittin derived peptides for nanoparticle based siRNA transfection. Biomaterials. 34(12) : 3110-9, 2013.
• Pan D, Schirra CO, Wickline SA, Lanza GM. Multicolor computed tomographic molecular imaging with noncrystalline high-metal-density nanobeacons. Contrast media & molecular imaging. 9(1) : 13-25, 2013.
• Hughes MS, McCarthy JE, Marsh JN, Wickline SA. Joint entropy of continuously differentiable ultrasonic waveforms. The Journal of the Acoustical Society of America. 133(1) : 283-300, 2013.

Awards/Honors

• Washington University Chancellors Award for Innovation and Entrepreneurship (Washington University - 2014)
• James R. Hornsby Family Professor of Biomedical Science (Washington University - 2012)
• Washington University Fellow Alumni Achievement Award (Washington University - 2012)
• Faculty Leader of the Year (Washington University - 2010)
• Established Investigator Award (American Heart Association - 1998)
• Clinician-Scientist Award (American Heart Association - 1993)
• Knowlton "Incentive for Excellence" Award (Washingon University School of Medicine - 1986)
• Lange Award for Outstanding Student sophomore, junior, and senior years; (John A. Burns School of Medicine - 1980)
• First Place - Presentation ofVectorcardiographic Infarct Sizing in Baboon (University of Hawaii - 1977)