Functional consequences of bacterial-fungal dysbiosis in E/VLBW infants- Study functional changes to the developing infant microbiome that are dependent on proper trans-kingdom interactions among bacterial and fungal communities. 

Chronic Toxoplasma gondii, Pregnancy reactivation, and Perinatal Depression- This study will contribute new knowledge to understandings of the pathophysiology of both T. gondii infection and depression in the perinatal period. The possible reactivation of the latent parasite will be examined in Hispanic women. We will explore the potential transmission of T.gondii to the fetus in mothers who are T.gondii positive.   

The Preterm Infant Microbiome: Biological, Behavioral and Health Outcomes at 2 and 4 years of Age- This is a prospective study of a cohort of VLBW infants initially studied in an R21 grant, with measures of human milk intake, milk immunobiology, fecal calprotectin levels and early health outcomes. We propose to study the microbiomes of these infants in the 6 weeks of preserved stool samples while they were admitted to the Neonatal Intensive Care Unit. We will follow these infants through 4 years of age, studying the succession of the microbiome, and the relationships of microbiome to early and later health outcomes. 

The Association between Preterm Milk Immunobiology and Infant Health- Study the relationships between human milk immunity and milk volume with clinical outcomes and immune and enteral biomarkers in preterm infants while hospitalized in the neonatal intensive care unit. 

The Administration of Time-Matched Breast Milk: Infant Sleep Protection and Promotion

The Impact of Maternal Sleep on Human Milk Oligosaccharides and Microbiome

Our volunteer program aims to provide a holistic biobehavioral laboratory experience for students with an interest in research and bench science. We host an array of volunteers, including both undergraduate and graduate students from different disciplines (nursing, pre-medical, biotechnology, psychology, etc.) with varying levels of experience. No prior laboratory experience is required to join—we hope to foster an interest and passion in biobehavioral research in students from any background, as well as promote a sense of community, collaboration, and empowerment in STEM.

Our volunteers receive instruction and training in a variety of bench science methods including DNA extraction & sequencing, RNA extraction & sequencing, cell culturing, ELISA and multiplex ELISA. Volunteers are also given the opportunity to participate in ongoing research within the College of Nursing. We encourage poster presentations and manuscript development/authorship! Volunteers may obtain leadership positions in the lab.

• Developing model for measuring glucose uptake in intestinal epithelial cells.
• Developing protocols demonstrating reliability, validity, and internal consistency of small volume samples for multi-omic studies including: 
  • Rectal swab stored at room temperature vs stool frozen at -80 degrees.
  • Dried blood spot vs. Serum for protein and cytokine analysis.
• Determine liver cell expression differences by gender in response to western and regular diet.
• Determine mesenteric fat expression differences by gender in response to western and regular diet.
• Determine impact of microbiota on growth in infants with postnatal growth failure.

• llumina MiSeq desktop gene sequencer
• MAGPIX multiplexing system
• QuantStudio 5 Real-Time PCR system
• Thermo Scientific Multiskan FC (ELISA microplate reader)
• (x6) -80o Freezers
• Liquid nitrogen tanks (cell storage), cell culture room
• (x3) Fume hoods