Molecular Pharmacology and Physiology

Craig Doupnik I-3617-2012

Craig Doupnik, Ph.D.

Asst Dean, College of Medicine Molecular Pharmacology & Physiology

Associate Professor (Tenured), Department of Molecular Pharmacology & Physiology, College of Medicine

Contact Info 12901 Bruce B. Downs Blvd.
Tampa FL 33612

Academic Email:

Academic Phone:(813) 974-1557


  • PHD, Physiology & Biophysics, University of Cincinnati, 1993
  • M.Sc., Physiology & Biophysics, University of Cincinnati, 1989
  • B.Sc., Biological Sciences, Ohio State University, 1984

Interdisciplinary and Emerging Signature Programs

  • Biomedical Engineering & Nanomedicine
  • Cardiovascular Sciences
  • Neuroscience

Research Interests

  • 1) Ion channel regulation by G protein signaling networks, 2) Computational biology & protein engineering, 3) Venom peptides


  • American Chemical Society (member, 2020 - Present)
  • International Society on Toxinology (member, 2015 - Present)
  • American Peptide Society (member, 2015 - Present)
  • International Association of Medical Science Educators (member, 2011 - Present)

Recent Publications

  • Patel D, Kuyucak S, Doupnik CA. Structural Determinants Mediating Tertiapin Block of Neuronal Kir3.2 Channels. Biochemistry. , 2020.
  • Doupnik CA. Identification of Aethina tumida Kir Channels as Putative Targets of the Bee Venom Peptide Tertiapin Using Structure-Based Virtual Screening Methods. Toxins. 11(9) , 2019.
  • Doupnik CA. Venom-derived peptides inhibiting Kir channels: Past, present, and future. Neuropharmacology. 127: 161-172, 2017.
  • Doupnik CA. RGS Redundancy and Implications in GPCR-GIRK Signaling. International Review of Neurobiology. 123: 87-116, 2015.
  • Doupnik CA, Parra KC, & Guida WC. A computational design approach for virtual screening of peptide interactions across K(+) channel families. Computational and Structural Biotechnology Journal. 13: 85-94, 2014.
  • Seyfang Nazian SJ, Saporta S, Doupnik CA, Johnson WE, & Stevenson FT. Assessment of an adjusted vs. fixed pass line for student performance in a medical school curriculum. Medical Science Educator. 22: 270-271, 2012.
  • Doupnik CA. GPCR-Kir channel signaling complexes: defining rules of engagement. Journal of receptor and signal transduction research. 28(1-2) : 83-91, 2008.
  • Drenan RM, Doupnik CA, Jayaraman M, Buchwalter AL, Kaltenbronn KM, Huettner JE, Linder ME, Blumer KJ. R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism. The Journal of biological chemistry. 281(38) : 28222-31, 2006.
  • Jaén C, Doupnik CA. RGS3 and RGS4 differentially associate with G protein-coupled receptor-Kir3 channel signaling complexes revealing two modes of RGS modulation. Precoupling and collision coupling. The Journal of biological chemistry. 281(45) : 34549-60, 2006.
  • Jaén C, Doupnik CA. Neuronal Kir3.1/Kir3.2a channels coupled to serotonin 1A and muscarinic m2 receptors are differentially modulated by the "short" RGS3 isoform. Neuropharmacology. 49(4) : 465-76, 2005.
  • Drenan RM, Doupnik CA, Boyle MP, Muglia LJ, Huettner JE, Linder ME, Blumer KJ. Palmitoylation regulates plasma membrane-nuclear shuttling of R7BP, a novel membrane anchor for the RGS7 family. The Journal of cell biology. 169(4) : 623-33, 2005.
  • Doupnik CA, Jaén C, Zhang Q. Measuring the modulatory effects of RGS proteins on GIRK channels. Methods in enzymology. 389: 131-54, 2004.
  • Zhang Q, Dickson A, Doupnik CA. Gbetagamma-activated inwardly rectifying K(+) (GIRK) channel activation kinetics via Galphai and Galphao-coupled receptors are determined by Galpha-specific interdomain interactions that affect GDP release rates. The Journal of biological chemistry. 279(28) : 29787-96, 2004.
  • Zhang Q, Pacheco MA, Doupnik CA. Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation. The Journal of physiology. 545(Pt 2) : 355-73, 2002.
  • Doupnik CA, Xu T, Shinaman JM. Profile of RGS expression in single rat atrial myocytes. Biochimica et biophysica acta. 1522(2) : 97-107, 2001.
  • Cunnick JM, Mei L, Doupnik CA, Wu J. Phosphotyrosines 627 and 659 of Gab1 constitute a bisphosphoryl tyrosine-based activation motif (BTAM) conferring binding and activation of SHP2. The Journal of biological chemistry. 276(26) : 24380-7, 2001.
  • Ehrengruber MU, Doupnik CA, Xu Y, Garvey J, Jasek MC, Lester HA, Davidson N. Activation of heteromeric G protein-gated inward rectifier K+ channels overexpressed by adenovirus gene transfer inhibits the excitability of hippocampal neurons. Proceedings of the National Academy of Sciences of the United States of America. 94(13) : 7070-5, 1997.
  • Doupnik CA, Davidson N, Lester HA, Kofuji P. RGS proteins reconstitute the rapid gating kinetics of gbetagamma-activated inwardly rectifying K+ channels. Proceedings of the National Academy of Sciences of the United States of America. 94(19) : 10461-6, 1997.
  • Kofuji P, Doupnik CA, Davidson N, Lester HA. A unique P-region residue is required for slow voltage-dependent gating of a G protein-activated inward rectifier K+ channel expressed in Xenopus oocytes. The Journal of physiology. 490 ( Pt 3): 633-45, 1996.
  • Doupnik CA, Dessauer CW, Slepak VZ, Gilman AG, Davidson N, Lester HA. Time resolved kinetics of direct G beta 1 gamma 2 interactions with the carboxyl terminus of Kir3.4 inward rectifier K+ channel subunits. Neuropharmacology. 35(7) : 923-31, 1996.
  • Doupnik CA, Lim NF, Kofuji P, Davidson N, Lester HA. Intrinsic gating properties of a cloned G protein-activated inward rectifier K+ channel. The Journal of general physiology. 106(1) : 1-23, 1995.
  • Doupnik CA, Davidson N, Lester HA. The inward rectifier potassium channel family. Current opinion in neurobiology. 5(3) : 268-77, 1995.
  • Dascal N, Doupnik CA, Ivanina T, Bausch S, Wang W, Lin C, Garvey J, Chavkin C, Lester HA, Davidson N. Inhibition of function in Xenopus oocytes of the inwardly rectifying G-protein-activated atrial K channel (GIRK1) by overexpression of a membrane-attached form of the C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America. 92(15) : 6758-62, 1995.
  • Doupnik CA, Pun RY. G-protein activation mediates prepulse facilitation of Ca2+ channel currents in bovine chromaffin cells. The Journal of membrane biology. 140(1) : 47-56, 1994.
  • Doupnik CA, Pun RY. Cyclic AMP-dependent phosphorylation modifies the gating properties of L-type Ca2+ channels in bovine adrenal chromaffin cells. Pflügers Archiv : European journal of physiology. 420(1) : 61-71, 1992.
  • Plews PI, Abdel-Malek ZA, Doupnik CA, Leikauf GD. Endothelin stimulates chloride secretion across canine tracheal epithelium. The American journal of physiology. 261(2 Pt 1) : L188-94, 1991.
  • Doupnik CA, Leikauf GD. Acrolein stimulates eicosanoid release from bovine airway epithelial cells. The American journal of physiology. 259(4 Pt 1) : L222-9, 1990.
  • Lee HK, Ohya Y, Doupnik CA, Sperelakis N. Effects of azelastine on contraction of guinea pig tracheal smooth muscle. European journal of pharmacology. 187(1) : 67-74, 1990.
  • Leikauf GD, Claesson HE, Doupnik CA, Hybbinette S, Grafström RC. Cysteinyl leukotrienes enhance growth of human airway epithelial cells. The American journal of physiology. 259(4 Pt 1) : L255-61, 1990.
  • Leikauf GD, Leming LM, O'Donnell JR, Doupnik CA. Bronchial responsiveness and inflammation in guinea pigs exposed to acrolein. Journal of applied physiology (Bethesda, Md. : 1985). 66(1) : 171-8, 1989.
  • Murlas CG, Doupnik CA. Electromechanical coupling of ferret airway smooth muscle in response to leukotriene C4. Journal of applied physiology (Bethesda, Md. : 1985). 66(6) : 2533-8, 1989.
  • Leikauf GD, Doupnik CA, Leming LM, Wey HE. Sulfidopeptide leukotrienes mediate acrolein-induced bronchial hyperresponsiveness. Journal of applied physiology (Bethesda, Md. : 1985). 66(4) : 1838-45, 1989.

Positions Held

  • Assistant Professor (Department of Physiology & Biophysics, College of Medicine, University of South Florida 1997 - 2003)
  • Postdoctoral Fellow (Biology, California Institute of Technology 1993 - 1997)


  • Dean's Recognition Award for Teaching Excellence (University of South Florida College of Medicine - 2013)
  • Robert J. Grasso Award (Association of Medical Science Graduate Students (AMSGS) - 2003)
  • Robert J. Boucek Research Award (American Heart Association - Florida & Puerto Rico Affiliate - 1998)
  • Initial Investigator Award (American Heart Association - Florida & Puerto Rico Affiliate - 1998)
  • Postdoctoral Fellowship Awards (American Heart Association - Greater Los Angeles Affiliate - 1994)
  • Jeffery Doane Memorial Award (University of Cincinnati College of Medicine - 1991)