Molecular Pharmacology and Physiology

David E Birk I-4072-2012

David E Birk, PH.D.

Professor, Orthopaedics & Sports Medicine, College of Medicine

Distinguished Professor, Molecular Pharmacology & Physiology

Adjunct Professor, Orthopaedic Surgery, Perelman School of Medicine

Contact Info Department of Molecular Pharmacology & Physiology
12901 Bruce B. Downs Blvd., MDC 8
Tampa FL 33612

Academic Email: dbirk@health.usf.edu

Academic Phone:(813) 974-8598

View My C.V.

Education

  • PHD, Anatomy, Wayne State University, 1981
  • MS, Biology, New York University, 1978
  • AB, Biology, Colgate University, 1973

Interdisciplinary and Emerging Signature Programs

  • Allergy, Immunology & Infectious Disease
  • Cellular and Molecular Biology

Research Interests

  • Our general research interests are the cell and molecular biology as well as pathobiology of the extracellular matrix. Our major focus is on acquisition and reacquisition of tissue-specific structure and function. We utilize a multidisciplinary approach integrating genetics and mouse models with cell, and molecular biology as well as biomechanics to elucidate the regulatory mechanisms involved in development, growth and aging as well as the relationship between a dysfunctional regulatory step and pathobiology. Our current work focuses on the regulatory roles of small leucine rich proteoglycans, collagen V/XI, as well as collagens XII and XIV in the regulation of matrix assembly. Our main projects address extracellular matrix in the dermis, tendons, ligaments and the corneal stroma. This work also addresses the molecular pathophysiology underlying human congenital disorders such as, Ehlers Danlos syndrome, congenital corneal stromal dystrophy, myopathies and osteogenesis imperfecta.

Memberships

  • Orthopaedic Research Society (Member, 2009 - Present)
  • American Society for Biochemistry and Molecular Biology (Member, 2008 - Present)
  • American Society for Matrix Biology (Member, (Council 2014-2017), 1998 - Present)
  • American Association of Anatomists (Member, 1981 - Present)
  • Association for Research in Vision and Opthhalmology (Member, 1979 - Present)
  • American Society for Investigative Pathology (Member, 2014 - 2016)
  • International Society for Matrix Biology (Member, (Council 2009-2012), 1986 - 2013)

Recent Publications

  • Hubmacher D, Taye N, Balic Z, Thacker S, Adams SM, Birk DE, Schweitzer R, Apte SS. Limb- and tendon-specific Adamtsl2 deletion identifies a role for ADAMTSL2 in tendon growth in a mouse model for geleophysic dysplasia. Matrix biology : journal of the International Society for Matrix Biology. , 2019. http://www.ncbi.nlm.nih.gov/pubmed/30738849
  • Call M, Elzarka M, Kunesh M, Hura N, Birk DE, Kao WW. Therapeutic efficacy of mesenchymal stem cells for the treatment of congenital and acquired corneal opacity. Molecular vision. 25: 415-426, 2019. http://www.ncbi.nlm.nih.gov/pubmed/31523119
  • Han B, Li Q, Wang C, Patel P, Adams SM, Doyran B, Nia HT, Oftadeh R, Zhou S, Li CY, Liu XS, Lu XL, Enomoto-Iwamoto M, Qin L, Mauck RL, Iozzo RV, Birk DE, Han L. Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix. ACS nano. 13(10) : 11320-11333, 2019. http://www.ncbi.nlm.nih.gov/pubmed/31550133
  • Mead TJ, McCulloch DR, Ho JC, Du Y, Adams SM, Birk DE, Apte SS. The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate, tendon-specific protective mechanism against heterotopic ossification. JCI insight. 3(7) , 2018. http://www.ncbi.nlm.nih.gov/pubmed/29618652
  • Baghdadi MB, Castel D, Machado L, Fukada SI, Birk DE, Relaix F, Tajbakhsh S, Mourikis P. Reciprocal signalling by Notch-Collagen V-CALCR retains muscle stem cells in their niche. Nature. 557(7707) : 714-718, 2018. http://www.ncbi.nlm.nih.gov/pubmed/29795344
  • Robinson KA, Sun M, Barnum CE, Weiss SN, Huegel J, Shetye SS, Lin L, Saez D, Adams SM, Iozzo RV, Soslowsky LJ, Birk DE. Decorin and biglycan are necessary for maintaining collagen fibril structure, fiber realignment, and mechanical properties of mature tendons. Matrix biology : journal of the International Society for Matrix Biology. 64: 81-93, 2017. http://www.ncbi.nlm.nih.gov/pubmed/28882761
  • Park AC, Phan N, Massoudi D, Liu Z, Kernien JF, Adams SM, Davidson JM, Birk DE, Liu B, Greenspan DS. Deficits in Col5a2 Expression Result in Novel Skin and Adipose Abnormalities and Predisposition to Aortic Aneurysms and Dissections. The American journal of pathology. 187(10) : 2300-2311, 2017. http://www.ncbi.nlm.nih.gov/pubmed/28734943
  • Johnston JM, Connizzo BK, Shetye SS, Robinson KA, Huegel J, Rodriguez AB, Sun M, Adams SM, Birk DE, Soslowsky LJ. Collagen V haploinsufficiency in a murine model of classic Ehlers-Danlos syndrome is associated with deficient structural and mechanical healing in tendons. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 35(12) : 2707-2715, 2017. http://www.ncbi.nlm.nih.gov/pubmed/28387435
  • Foster JW, Wahlin K, Adams SM, Birk DE, Zack DJ, Chakravarti S. Cornea organoids from human induced pluripotent stem cells. Scientific reports. 7: 41286, 2017. http://www.ncbi.nlm.nih.gov/pubmed/28128337
  • Muir AM, Massoudi D, Nguyen N, Keene DR, Lee SJ, Birk DE, Davidson JM, Marinkovich MP, Greenspan DS. BMP1-like proteinases are essential to the structure and wound healing of skin. Matrix biology : journal of the International Society for Matrix Biology. 56: 114-131, 2016. http://www.ncbi.nlm.nih.gov/pubmed/27363389
  • Connizzo BK, Adams SM, Adams TH, Jawad AF, Birk DE, Soslowsky LJ. Multiscale regression modeling in mouse supraspinatus tendons reveals that dynamic processes act as mediators in structure-function relationships. Journal of biomechanics. 49(9) : 1649-57, 2016. http://www.ncbi.nlm.nih.gov/pubmed/27067362
  • Connizzo BK, Han L, Birk DE, Soslowsky LJ. Collagen V-heterozygous and -null supraspinatus tendons exhibit altered dynamic mechanical behaviour at multiple hierarchical scales. Interface focus. 6(1) : 20150043, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26855746
  • DeNigris J, Yao Q, Birk EK, Birk DE. Altered dermal fibroblast behavior in a collagen V haploinsufficient murine model of classic Ehlers-Danlos syndrome. Connective tissue research. 57(1) : 1-9, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26713685
  • Markova DZ, Pan TC, Zhang RZ, Zhang G, Sasaki T, Arita M, Birk DE, Chu ML. Forelimb contractures and abnormal tendon collagen fibrillogenesis in fibulin-4 null mice. Cell and tissue research. 364(3) : 637-46, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26711913
  • Izu Y, Ezura Y, Koch M, Birk DE, Noda M. Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis. Cell and tissue research. 364(3) : 623-35, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26753503
  • Connizzo BK, Adams SM, Adams TH, Birk DE, Soslowsky LJ. Collagen V expression is crucial in regional development of the supraspinatus tendon. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 34(12) : 2154-2161, 2016. http://www.ncbi.nlm.nih.gov/pubmed/28005290
  • Mienaltowski MJ, Dunkman AA, Buckley MR, Beason DP, Adams SM, Birk DE, Soslowsky LJ. The injury response of geriatric mouse patellar tendons. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. , 2015. http://www.ncbi.nlm.nih.gov/pubmed/26704368
  • Igoucheva O, Alexeev V, Halabi CM, Adams SM, Stoilov I, Sasaki T, Arita M, Donahue A, Mecham RP, Birk DE, Chu ML. Fibulin-4 E57K Knock-in Mice Recapitulate Cutaneous, Vascular and Skeletal Defects of Recessive Cutis Laxa 1B with both Elastic Fiber and Collagen Fibril Abnormalities. The Journal of biological chemistry. 290(35) : 21443-59, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26178373
  • Park AC, Phillips CL, Pfeiffer FM, Roenneburg DA, Kernien JF, Adams SM, Davidson JM, Birk DE, Greenspan DS. Homozygosity and Heterozygosity for Null Col5a2 Alleles Produce Embryonic Lethality and a Novel Classic Ehlers-Danlos Syndrome-Related Phenotype. The American journal of pathology. 185(7) : 2000-11, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25987251
  • Sun M, Connizzo BK, Adams SM, Freedman BR, Wenstrup RJ, Soslowsky LJ, Birk DE. Targeted deletion of collagen V in tendons and ligaments results in a classic Ehlers-Danlos syndrome joint phenotype. The American journal of pathology. 185(5) : 1436-47, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25797646
  • Chen S, Mienaltowski MJ, Birk DE. Regulation of corneal stroma extracellular matrix assembly. Experimental eye research. 133: 69-80, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25819456
  • Connizzo BK, Freedman BR, Fried JH, Sun M, Birk DE, Soslowsky LJ. Regulatory role of collagen V in establishing mechanical properties of tendons and ligaments is tissue dependent. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 33(6) : 882-8, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25876927
  • Gordon JA, Freedman BR, Zuskov A, Iozzo RV, Birk DE, Soslowsky LJ. Achilles tendons from decorin- and biglycan-null mouse models have inferior mechanical and structural properties predicted by an image-based empirical damage model. Journal of biomechanics. 48(10) : 2110-5, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25888014
  • Screen HR, Birk DE, Kadler KE, Ramirez F, Young MF. Tendon Functional Extracellular Matrix. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 33: 793-799, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25640030
  • Espana EM, Sun M, Birk DE. Existence of Corneal Endothelial Slow-Cycling Cells. Investigative ophthalmology & visual science. 56(6) : 3827-37, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26066751
  • Mienaltowski MJ, Adams SM, Birk DE. Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties. Stem cell research & therapy. 5(4) : 86, 2014. http://www.ncbi.nlm.nih.gov/pubmed/25005797
  • Chiquet M, Birk DE, Bönnemann CG, Koch M. Collagen XII: Protecting bone and muscle integrity by organizing collagen fibrils. The international journal of biochemistry & cell biology. 53C: 51-54, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24801612
  • Pan TC, Zhang RZ, Arita M, Bogdanovich S, Adams SM, Gara SK, Wagener R, Khurana TS, Birk DE, Chu ML. A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16. The Journal of biological chemistry. 289(15) : 10293-307, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24563484
  • Chen S, Young MF, Chakravarti S, Birk DE. Interclass small leucine-rich repeat proteoglycan interactions regulate collagen fibrillogenesis and corneal stromal assembly. Matrix biology : journal of the International Society for Matrix Biology. 35: 103-11, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24447998
  • Mienaltowski MJ, Birk DE. Structure, physiology, and biochemistry of collagens. Advances in experimental medicine and biology. 802: 5-29, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24443018
  • Basu S, Hertsenberg AJ, Funderburgh ML, Burrow MK, Mann MM, Du Y, Lathrop KL, Syed-Picard FN, Adams SM, Birk DE, Funderburgh JL. Human limbal biopsy-derived stromal stem cells prevent corneal scarring. Science translational medicine. 6(266) : 266ra172, 2014. http://www.ncbi.nlm.nih.gov/pubmed/25504883
  • Muir AM, Ren Y, Butz DH, Davis NA, Blank RD, Birk DE, Lee SJ, Rowe D, Feng JQ, Greenspan DS. Induced ablation of Bmp1 and Tll1 produces osteogenesis imperfecta in mice. Human molecular genetics. 23(12) : 3085-101, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24419319
  • Zou Y, Zwolanek D, Izu Y, Gandhy S, Schreiber G, Brockmann K, Devoto M, Tian Z, Hu Y, Veit G, Meier M, Stetefeld J, Hicks D, Straub V, Voermans NC, Birk DE, Barton ER, Koch M, Bönnemann CG. Recessive and dominant mutations in COL12A1 cause a novel EDS/myopathy overlap syndrome in humans and mice. Human molecular genetics. 23(9) : 2339-52, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24334604
  • Dunkman AA, Buckley MR, Mienaltowski MJ, Adams SM, Thomas SJ, Satchell L, Kumar A, Pathmanathan L, Beason DP, Iozzo RV, Birk DE, Soslowsky LJ. The tendon injury response is influenced by decorin and biglycan. Annals of biomedical engineering. 42(3) : 619-30, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24072490
  • Dunkman AA, Buckley MR, Mienaltowski MJ, Adams SM, Thomas SJ, Kumar A, Beason DP, Iozzo RV, Birk DE, Soslowsky LJ. The injury response of aged tendons in the absence of biglycan and decorin. Matrix biology : journal of the International Society for Matrix Biology. 35: 232-8, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24157578
  • Smith SM, Zhang G, Birk DE. Collagen V localizes to pericellular sites during tendon collagen fibrillogenesis. Matrix biology : journal of the International Society for Matrix Biology. 33: 47-53, 2014. http://www.ncbi.nlm.nih.gov/pubmed/23954412
  • Mienaltowski MJ, Birk DE. Mouse models in tendon and ligament research. Advances in experimental medicine and biology. 802: 201-30, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24443029

Positions Held

  • Professor (Molecular Pharmacology and Physiology, University of South Florida 2012 - 2015)
  • Professor (Pathology & Cell Biology, College of Medicine, University of South Florida 2007 - 2012)
  • Professor (Pathology, Anatomy & Cell Biology, Jefferson Medical College, Thomas Jefferson University 1998 - 2008)
  • Professor (Ophthalmology, Tufts University School of Medicine 1997 - 1998)
  • Professor (Anatomy and Cellular Biology, Tufts University School of Medicine 1996 - 1998)
  • Associate Professor (Anatomy and Cellular Biology, Tufts University School of Medicine 1992 - 1996)
  • Graduate Faculty (Cell and Developmental Biology Program, Rutgers University 1984 - 1992)
  • Associate Professor (Pathology, University of Medicine & Dentistry of New Jersey-Robert Wood Johnson Medical School 1989 - 1992)
  • Assistant Professor (Pathology, University of Medicine & Dentistry of New Jersey-Robert Wood Johnson Medical School 1984 - 1989)

Awards/Honors

  • Fellow (American Association of Anatomists - 2015)
  • Fellow (Association for Research in Vision and Ophthalmology - 2010)
  • Dean's Citation for Significant Contributions to the Advancement of Education (Jefferson Medical College - 2006)
  • Excellence in Teaching Award (Awarded by Jefferson Medical College Class of 2008 - 2005)
  • Research Career Development Award (NIH NEI - 1984)
  • Individual NRSA (NIH NEI - 1982)