Flow Cytometry

Available Equipment

Canto II 405 LASER (60mW)
Detector LP  Band Pass Fluors
A 502  510/50 BV510, BD Horizon 500, Am Cyan
B none  450/50 DAPI, BV421, Pac Blue, BD Horizon V450

Canto II 488 LASER (20mW)
Detector LP  Band Pass Fluors
A 735  780/60 PE-CY7
B 655  670/LP 7AAD, PE-CY5, PerCP, PerCP-Cy5.5, PE-Cy5.5
C 610  none No PMT
D 556  585/42 Alexa532, DsRed, CytoxOr, PE, PI, EB
E 502  530/30 eYFP, FITC,  Alexa 488,  GFP, To-Pro-1, Cyto-ID
F none  488/10 SSC

 Canto II 633 LASER (17mW)
Detector LP  Band Pass Fluors
A 735  780/60 APC-CY7, Alexa750
B 685  - No PMT
C 660/20 APC, Alexa 647, CPC, Cy5, To-PRO-3, DRAQ5, DRAQ7

LSR II 405 LASER (25 mW)
Detector  LP  Band Pass Fluors
A 755  780/60 BV785
B 690  710/50 BV710
C 635  660/20 Qdot 655, BV650
D 595  610/20 Qdot 605, BV605
E 575  585/42 Qdot 585, BV570
F 505  525/50 BV510, BD Horizon 500, Am Cyan
G none  450/50 DAPI, BV421, Pac Blue, BD Horizon V450

LSR II 488 LASER (20 mW)
Detector LP  Band Pass Fluors
A 675  685/30 PerCP, PerCP-Cy5.5
B 505  510/20 FITC, Alexa 488,  eGFP, SyBRGreen, Cyto-ID
C none  488/10 SSC 

LSR II 553 LASER (50mW)
Detector LP Band Pass Fluors
A 747 780/60 PE-Cy7
B 680 710/50 PE-Cy5.5
C 633 666/27 PE-Cy5, mPlum
D 603 615/25 PE-Tx-Red, mRaspberry, Alexa568, TxRed
E none 580/20 PE, mTangerine, mOrange

LSR II 637 LASER (50mW)
Detector LP Band Pass Fluors
A 755 780/60 APC-Cy7
B 685 730/45 Alexa 700
C none 660/20 APC, Alexa 647, DRAQ5, DRAQ7

FacsAria IIu 488nm LASER
Detector LP Band Pass Fluors
A

635

660/20 PercpCy5.5
B 505 530/30 FITC, A488, GFP
C  none  488/10  SCC

FacsAria IIu 405nm LASER
Detector LP Band Pass Fluors
A 750 780/60 BV785
B 635 660/20 BV650
C 600 610/20 BV605
D 575 585/42 BV570
E 505 525/20 BV510
F none 450/50  BV421, DAPI

FacsAria Iiu 640nm LASER
Detector LP Band Pass Fluors
A 755 780/60 APC-CY7
B 710 730/45 A700
C none 670/14 APC, A647

FacsAria Iiu 355nm LASER
Detector LP Band Pass Fluors
A 505 525/50 BUV496
B none 450/50 DAPI

FacsAria Iiu 561nm LASER
Detector LP Band Pass Fluors
A 750 780/60 PE-Cy7
B 635 670/30 PE-Cy5
C 595 610/20 PE-TxRed, mCherry
D none 586/15 PE, PI

FacsMelody 488nm LASER
Detector LP Band Pass Fluors
A 752 783/56 PE-Cy7
B 665 700/54 PE-Cy5, PercpCy5.5, 7AAD
C 560 586/42 PE, PI
D 507 527/32 FITC, A488, GFP, BB515
E none 488/10 SSC

FacsMelody 405nm LASER
 Detector LP Band Pass Fluors 
A none 755LP DAPI, BV421
B 635 500LP BV510
C none 448/45 BV786

FacsMelody 640nm LASER
Detector LP Band Pass Fluors
A 752 783/56 APC-CY7
B none 660/10 APC-A647