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Flow Cytometry Core

Available Equipment

Canto II 405 LASER (60mW)
Detector LP Band Pass Fluors
A 502 510/50 BV510, BD Horizon 500, Am Cyan
B none 450/50 DAPI, BV421, Pac Blue, BD Horizon V450

Canto II 488 LASER (20mW)
Detector LP Band Pass Fluors
A 735 780/60 PE-CY7
B 655 670/LP 7AAD, PE-CY5, PerCP, PerCP-Cy5.5, PE-Cy5.5
C 610 none No PMT
D 556 585/42 Alexa532, DsRed, CytoxOr, PE, PI, EB
E 502 530/30 eYFP, FITC, Alexa 488, GFP, To-Pro-1, Cyto-ID
F none 488/10 SSC

Canto II 633 LASER (17mW)
Detector LP Band Pass Fluors
A 735 780/60 APC-CY7, Alexa750
B 685 - No PMT
C - 660/20 APC, Alexa 647, CPC, Cy5, To-PRO-3, DRAQ5, DRAQ7

LSR II 405 LASER (25 mW)
Detector LP Band Pass Fluors
A 635 660/20 Qdot 655, BV650
B 595 610/20 Qdot 605, BV605
C 575 585/42 Qdot 585, BV570
D 505 525/50 BV510, BD Horizon 500, Am Cyan
E none 450/50 DAPI, BV421, Pac Blue, BD Horizon V450
F      
G      

This is the default configuration, but if the experiment needs it, we can reconfigure it to accommodate BV710 or BV785

LSR II 488 LASER (20 mW)
Detector LP Band Pass Fluors
A 675 685/30 PerCP, PerCP-Cy5.5
B 505 510/20 FITC, Alexa 488, eGFP, SyBRGreen, Cyto-ID
C none 488/10 SSC

LSR II 553 LASER (50mW)
Detector LP Band Pass Fluors
A 747 780/60 PE-Cy7
B 680 710/50 PE-Cy5.5
C 633 666/27 PE-Cy5, mPlum
D 603 615/25 PE-Tx-Red, mRaspberry, Alexa568, TxRed
E none 580/20 PE, mTangerine, mOrange

LSR II 637 LASER (50mW)
Detector LP Band Pass Fluors
A 755 780/60 APC-Cy7
B 685 730/45 Alexa 700
C none 660/20 APC, Alexa 647, DRAQ5, DRAQ7

FacsAria IIu 488nm LASER
Detector LP Band Pass Fluors
A

635

 660/20 PercpCy5.5
B 505 530/30 FITC, A488, GFP
C none 488/10 SCC

FacsAria IIu 405nm LASER
Detector LP Band Pass Fluors
A 750 780/60 BV785
B 635 660/20 BV650
C 600 610/20 BV605
D 575 585/42 BV570
E 505 525/20 BV510
F none 450/50 BV421, DAPI

FacsAria Iiu 640nm LASER
Detector LP Band Pass Fluors
A 755 780/60 APC-CY7
B 710 730/45 A700
C none 670/14 APC, A647

FacsAria Iiu 355nm LASER
Detector LP Band Pass Fluors
A 505 525/50 BUV496
B none 450/50 DAPI

FacsAria Iiu 561nm LASER
Detector LP Band Pass Fluors
A 750 780/60 PE-Cy7
B 635 670/30 PE-Cy5
C 595 610/20 PE-TxRed, mCherry
D none 586/15 PE, PI


BD S8 FacsDiscover

Excitation: 405 nm 50 mW, 488 nm 100 mW, 561nm 50mW, 637 nm 100 mW

Detectors: 405 nm 20, 410-860nm; 488nm,16, 495-860 nm; 561 nm, 12, 570-860 nm; 637nm 8, 645-860nm

Imaging detectors are off the blue laser (even if the fluor would be better excited elsewhere), FL1: 434/46, FL2: 600/60, FL3 788/225. The imaging PMTs are NOT unmixed/compensated, so if you want to use these, make sure that no other fluor shows up here.

Our instrument does not have UV laser, nut we extensively tested it and the following BUVs can be used: 496, 563, 615, 661, 737, 805. Unmixing worked fine for nearly all combinations, with BUV615 have a few issues. If you are using more, make sure that you have brilliant staining buffer in the mix.