The USF Genomics Program offers three of training workshops, the RNA-seq Illumina Sequencing Laboratory Workshop, the RNA-seq Data Analysis Computational Workshop, and the Microbiome Analysis Training. The RNA-seq workshops are offered three times a year in February, May, and September. The first Microbiome Analysis Training will be offered in November 2019. These workshops are open to all in the USF community. The RNA-seq Illumnia Sequencing Laboratory Workshop is required for access to use the USF Genomics Equipment Core (more information here).
This training course includes presentations on technology theory and practical experience with entire laboratory sample prep workflow. Experienced trainers will lead you through using TapeStation and Miseq equipment. By the end of this training, you will be able to: complete Illumina library preparation process and set up a sequencing run using Illumina Miseq Control Software.
This 5-day in-depth course will instruct participants on the theoretical and practical concepts related to RNA sequencing analysis, enabling them to perform these analyses independently.
The first workshop module is an introduction to data analysis using Linux, assuring that all participants are able to follow the practical parts. The second module discusses advantages and disadvantages of current sequencing technologies and their implications on data analysis. The third module introduces preprocessing raw reads, read mapping, visualization of mapped reads and one proceeds with first hands-on analyses (QC, mapping, visualization). The fourth module introduces ‘R’ a programming language to perform statistical computing and visualization, and ‘Bioconductor’ an R based toolshed for analyzing genomic data. The last module addresses quantification of gene expression and predict differentially expressed genes, followed by a whole day hands-on RNA-Seq analysis pipeline: TUXEDO protocol.
Day 1 (Monday Morning): Illumina Sequencing Introduction & Computer and Software Check (open to non-registered participants)
Day 2 (Friday): Illumina Sequencing Refresher & Linux for Bioinformatics
Day 3 (Monday): Introduction to RNA sequencing & RNA-seq Alignment and Visualization
Day 4 (Tuesday): Introduction to R and Bioconductor & Expression and Differential Expression
Day 5 (Wednesday): RNA-seq - TUXEDO Pipeline Hands-on Workshop Structure
If you have questions regarding the USF Genomics Workshops, email email@example.com.
Due to enrollment caps, there is a waitlist for the USF Genomics Workshops. You will be contacted after registering about the next available workshop.
This 3-Day in-depth course will instruct participants on theoretical and practical concepts related to microbiome sequencing analysis, enabling them to perform these analyses independently.
Day 1: Introduction to Microbiome Data Analysis and Introduction to R and Data-Plotting
Day 2: Metataxonomic analysis Experimental Design and Metataxonomic analysis OTU/ASV Generation
Day 3: Metataxonomic analysis Visualization
A basic knowledge of biochemistry and molecular biology, a basic familiarity with computer usage, and a need for microbiome sequence analysis for current ongoing research projects are recommended prerequisites.
Participants should bring laptops with a minimum of the following configuration: Mac - OS X 10.12 (64-bit) or Windows - 7 (64-bit)
The deadline for the November 2019 training is October 24, 2019. Admittance to the course is limited and requires registration.
Questions regarding the USF Genomics Training can be sent to firstname.lastname@example.org