State-of-the-art facilities and techniques for high-quality purification necessary for structural and functional characterization.

Cloning & Construct Design

Primer design, codon optimization, vector selection. Fusion tag selection (His, MBP, GST, fluorescent tags). Strategies for domain truncation, signal peptide engineering, mutagenesis for stability, incorporation of cleavage sites.

Expression Systems

Bacterial, insect, and mammalian expression. Membrane protein workflows.

Protein Purification (Chromatography)

Affinity, ion exchange, SEC; FPLC method development; polishing for structural biology-grade samples.

Quality Control & Sample Logistics

SDS-PAGE, SEC-MALS (where available), DLS, thermostability assays, and endotoxin testing. Buffer screening, storage conditions, aliquoting, and coordination with downstream cores.