Molecular Medicine

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* (Molecular Medicine Faculty Profile)

Gene Ness

Gene Ness, Ph.D.

Professor, Department of Molecular Medicine, College of Medicine

Associate Member, H. Lee Moffitt Cancer Research Institute

Contact Info

  • 12901 Bruce B. Downs Blvd.
    MDC 7
    Tampa FL 33612
  • Academic Email: gness@usf.edu

Education

  • PHD, Biochemistry, University of North Dakota, 1971
  • B.S., Chemistry, Bemidji State University, 1966

Interdisciplinary and Emerging Signature Programs

  • Cardiovascular

Awards/Honors

  • Mentor, Outstanding Dissertation Prize Winning Student 1999)
  • Professorial Excellence Award 1997)
  • Bronze Service Award (American Heart Association - Florida Affiliate - 1992)
  • Outstanding Scientist Medal (Florida Academy of Sciences - 1988)

Memberships

  • Member (Society for Experimental Biology and Medicine, 2007 - Present)
  • Member (American Heart Association, 2007 - Present)
  • Member (American Society of Biochemistry and Molecular Biology, 2007 - Present)

Recent Publications

  • Ness GC, Nazian SJ. Developmental expression of multiple forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA in rat testes. Journal of andrology. 13(4) : 318-22
  • Nguyen LB, Salen G, Shefer S, Tint GS, Shore V, Ness GC. Decreased cholesterol biosynthesis in sitosterolemia with xanthomatosis: diminished mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and enzyme protein associated with increased low-density lipoprotein receptor function. Metabolism: clinical and experimental. 39(4) : 436-43, 1990.
  • Simonet WS, Ness GC. Post-transcriptional regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA in rat liver. Glucocorticoids block the stabilization caused by thyroid hormones. The Journal of biological chemistry. 264(1) : 569-73, 1989.
  • Ness GC, Lalich JJ, Porter JW. Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in allylamine-fed rats. Atherosclerosis. 19(2) : 185-9
  • Ness GC, Sukalski KA, Sample CE, Pendleton LC, McCreery MJ, Nordlie RC. Radiation inactivation analysis of rat liver microsomal glucose-6-phosphatase. The Journal of biological chemistry. 264(13) : 7111-4, 1989.
  • Nazian SJ, Brewer LD, Ness GC. Pituitary regulation of the expression of the farnesyl pyrophosphate synthetase gene in the testes of the sexually maturing rat. Journal of andrology. 12(4) : 264-72
  • Ness GC, Pendleton LC, McCreery MJ. In situ determination of the functional size of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase by radiation inactivation analysis. Biochimica et biophysica acta. 953(3) : 361-4, 1988.
  • You M, Cao Q, Liang X, Ajmo JM, Ness GC. Mammalian sirtuin 1 is involved in the protective action of dietary saturated fat against alcoholic fatty liver in mice. The Journal of nutrition. 138(3) : 497-501, 2008.
  • Simonet WS, Ness GC. Transcriptional and posttranscriptional regulation of rat hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by thyroid hormones. The Journal of biological chemistry. 263(25) : 12448-53, 1988.
  • You M, Liang X, Ajmo JM, Ness GC. Involvement of mammalian sirtuin 1 in the action of ethanol in the liver. American journal of physiology. Gastrointestinal and liver physiology. 294(4) : G892-8, 2008.
  • Harwood HJ, Alvarez IM, Greene YJ, Ness GC, Stacpoole PW. Development of a noncompetitive, solid phase, bridged biotin-avidin enzyme immunoassay for measurement of human leukocyte microsomal HMG-CoA reductase protein concentration. Journal of lipid research. 28(3) : 292-304, 1987.
  • Lagor WR, Heller R, de Groh ED, Ness GC. Functional analysis of the hepatic HMG-CoA reductase promoter by in vivo electroporation. Experimental biology and medicine (Maywood, N.J.). 232(3) : 353-61, 2007.
  • Ness GC, Pendleton LC, Pendleton AS. Loss of NADPH during assays of HMG-CoA reductase: implications and approaches to minimize errors. Lipids. 22(6) : 409-12, 1987.
  • Eichler DC, Solomonson LP, Barber MJ, McCreery MJ, Ness GC. Radiation inactivation analysis of enzymes. Effect of free radical scavengers on apparent target sizes. The Journal of biological chemistry. 262(20) : 9433-6, 1987.
  • Sample CE, Pendleton LC, Ness GC. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA levels by L-triiodothyronine. Biochemistry. 26(3) : 727-31, 1987.
  • Lopez D, Abisambra Socarrás JF, Bedi M, Ness GC. Activation of the hepatic LDL receptor promoter by thyroid hormone. Biochimica et biophysica acta. 1771(9) : 1216-25, 2007.
  • Simonet WS, Ness GC. Turnover rates of HMG-CoA reductase mRNA: role of pituitary and thyroid hormones. Biochemical and biophysical research communications. 146(3) : 1033-9, 1987.
  • Ness GC, Holland RC, Lopez D. Selective compensatory induction of hepatic HMG-CoA reductase in response to inhibition of cholesterol absorption. Experimental biology and medicine (Maywood, N.J.). 231(5) : 559-65, 2006.
  • Sample CE, Ness GC. Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase by insulin. Biochemical and biophysical research communications. 137(1) : 201-7, 1986.
  • Lopez D, Ness GC. Characterization of the rat LDL receptor 5''-flanking region. Biochimica et biophysica acta. 1761(4) : 492-500, 2006.
  • Ness GC, Sample CE, Smith M, Pendleton LC, Eichler DC. Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase. The Biochemical journal. 233(1) : 167-72, 1986.
  • Ness GC, Holland RC. Degradation of HMG-CoA reductase in rat liver is cholesterol and ubiquitin independent. FEBS letters. 579(14) : 3126-30, 2005.
  • Ness GC, Eales SJ, Pendleton LC, Smith M. Activation of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by NADPH. Effects of dietary treatments. The Journal of biological chemistry. 260(23) : 12391-3, 1985.
  • Ness GC, McCreery MJ, Sample CE, Smith M, Pendleton LC. Sulfhydryl/disulfide forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Journal of biological chemistry. 260(30) : 16395-9, 1985.
  • Ness GC, Pendleton LC, McCreery MJ. Target size analysis by radiation inactivation: the use of free radical scavengers. Experimental biology and medicine (Maywood, N.J.). 230(7) : 455-63, 2005.
  • Lagor WR, de Groh ED, Ness GC. Diabetes alters the occupancy of the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase promoter. The Journal of biological chemistry. 280(44) : 36601-8, 2005.
  • Ness GC, Gertz KR. Hepatic HMG-CoA reductase expression and resistance to dietary cholesterol. Experimental biology and medicine (Maywood, N.J.). 229(5) : 412-6, 2004.
  • Singer II, Kawka DW, Kazazis DM, Alberts AW, Chen JS, Huff JW, Ness GC. Hydroxymethylglutaryl-coenzyme A reductase-containing hepatocytes are distributed periportally in normal and mevinolin-treated rat livers. Proceedings of the National Academy of Sciences of the United States of America. 81(17) : 5556-60, 1984.
  • Ness GC, Gertz KR. Increased sensitivity to dietary cholesterol in diabetic and hypothyroid rats associated with low levels of hepatic HMG-CoA reductase expression. Experimental biology and medicine (Maywood, N.J.). 229(5) : 407-11, 2004.
  • Phillips CE, Ness GC. Topography of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase. Biochemical and biophysical research communications. 119(2) : 772-8, 1984.
  • Osborne AR, Pollock VV, Lagor WR, Ness GC. Identification of insulin-responsive regions in the HMG-CoA reductase promoter. Biochemical and biophysical research communications. 318(4) : 814-8, 2004.
  • Ness GC. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Molecular and cellular biochemistry. 53-54(1-2) : 299-306, 1983.
  • Oettl K, Höfler G, Ness GC, Sattler W, Malle E. An apparent decrease in cholesterol biosynthesis in peroxisomal-defective Chinese hamster ovary cells is related to impaired mitochondrial oxidation. Biochemical and biophysical research communications. 305(4) : 957-63, 2003.
  • Ness GC, Benton GA, Deiter SA, Wickham PS. Influence of mevalonate kinase on studies of the MgATP-dependent inactivator of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Archives of biochemistry and biophysics. 214(2) : 705-13, 1982.
  • Ness GC, Kohlruss N, Gertz KR. Association of the low-density lipoprotein receptor with caveolae in hamster and rat liver. Biochemical and biophysical research communications. 303(1) : 177-81, 2003.
  • Ness GC, Way SC, Wickham PS. Proteinase involvement in the solubilization of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Biochemical and biophysical research communications. 102(1) : 81-5, 1981.
  • Fitzky BU, Moebius FF, Asaoka H, Waage-Baudet H, Xu L, Xu G, Maeda N, Kluckman K, Hiller S, Yu H, Batta AK, Shefer S, Chen T, Salen G, Sulik K, Simoni RD, Ness GC, Glossmann H, Patel SB, Tint GS. 7-Dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of Smith-Lemli-Opitz/RSH syndrome. The Journal of clinical investigation. 108(6) : 905-15, 2001.
  • Ness GC, Spindler CD, Benton GA. Characteristics of magnesium-adenosine triphosphate-dependent inactivators of 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Journal of biological chemistry. 255(19) : 9013-6, 1980.
  • Ness GC, Gertz KR, Holland RC. Regulation of hepatic lanosterol 14 alpha-demethylase gene expression by dietary cholesterol and cholesterol-lowering agents. Archives of biochemistry and biophysics. 395(2) : 233-8, 2001.
  • Ness GC, Spindler CD, Moffler MH. Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase from rat liver. Archives of biochemistry and biophysics. 197(2) : 493-9, 1979.
  • Ness GC, Chambers CM. Feedback and hormonal regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase: the concept of cholesterol buffering capacity. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 224(1) : 8-19, 2000.
  • Keller RK, Adair WL, Ness GC. Studies on the regulation of glycoprotein biosynthesis. An investigation of the rate-limiting steps of dolichyl phosphate biosynthesis. The Journal of biological chemistry. 254(20) : 9966-9, 1979.
  • Slakey LL, Ferrick TJ, Ness GC, Porter JW. De novo fatty acid synthesis and fatty acid elongation catalyzed by subcellular fractions from hog and human aorta. Lipids. 14(5) : 451-7, 1979.
  • Ness GC, Zhao Z, Wiggins L. Insulin and glucagon modulate hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity by affecting immunoreactive protein levels. The Journal of biological chemistry. 269(46) : 29168-72, 1994.
  • Ness GC, Moffler MH. Influence of a naturally occurring competing enzymic activity on studies of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Biochimica et biophysica acta. 572(2) : 333-44, 1979.
  • Ness GC, Chambers CM, Lopez D. Atorvastatin action involves diminished recovery of hepatic HMG-CoA reductase activity. Journal of lipid research. 39(1) : 75-84, 1998.
  • Ness GC, Miller JP, Moffler MH, Woods LS, Harris HB. Perinatal development of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in rat lung, liver and brain. Lipids. 14(5) : 447-50, 1979.
  • Ness GC. Developmental regulation of the expression of genes encoding proteins involved in cholesterol homeostasis. American journal of medical genetics. 50(4) : 355-7, 1994.
  • Lopez D, Chambers CM, Keller RK, Ness GC. Compensatory responses to inhibition of hepatic squalene synthase. Archives of biochemistry and biophysics. 351(2) : 159-66, 1998.
  • Ness GC, Moffler MH. Interference with the determination of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and its properties by a microsomal enzymic activity. Archives of biochemistry and biophysics. 189(1) : 221-3, 1978.
  • Xu G, Salen G, Shefer S, Batta AK, Ness GC, Nguyen LB, Zhao Z, Chen TS, Niemann W, Tint GS. Different feedback regulation of hepatic cholesterol and bile acid synthesis by glycodeoxycholic acid in rabbits. Gastroenterology. 105(4) : 1192-9, 1993.
  • Chambers CM, Ness GC. Dietary cholesterol regulates hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression in rats primarily at the level of translation. Archives of biochemistry and biophysics. 354(2) : 317-22, 1998.
  • Lakshmanan MR, Dugan RE, Nepokroeff CM, Ness GC, Porter JW. Regulation of rat liver beta-hydroxy-beta-methylglutaryl coenzyme. A reductase activity and cholesterol levels of serum and liver in various dietary and hormonal states. Archives of biochemistry and biophysics. 168(1) : 89-95, 1975.
  • Keller RK, Cannons A, Vilsaint F, Zhao Z, Ness GC. Identification and regulation of rat squalene synthetase mRNA. Archives of biochemistry and biophysics. 302(1) : 304-6, 1993.
  • Ness GC, Lopez D, Chambers CM, Zhao Z, Beach DL, Ko SS, Trzaskos JM. Effects of 15-oxa-32-vinyl-lanost-8-ene-3 beta,32 diol on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase and low density lipoprotein receptor in rat liver. Archives of biochemistry and biophysics. 357(2) : 259-64, 1998.
  • Dugan RE, Ness GC, Lakshmanan MR, Nepokroeff CM, Porter JW. Regulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase by the interplay of hormones. Archives of biochemistry and biophysics. 161(2) : 499-504, 1974.
  • Shefer S, Nguyen LB, Salen G, Ness GC, Chowdhary IR, Lerner S, Batta AK, Tint GS. Differing effects of cholesterol and taurocholate on steady state hepatic HMG-CoA reductase and cholesterol 7 alpha-hydroxylase activities and mRNA levels in the rat. Journal of lipid research. 33(8) : 1193-200, 1992.
  • Ness GC, Lopez D, Chambers CM, Newsome WP, Cornelius P, Long CA, Harwood HJ. Effects of L-triiodothyronine and the thyromimetic L-94901 on serum lipoprotein levels and hepatic low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and apo A-I gene expression. Biochemical pharmacology. 56(1) : 121-9, 1998.
  • Salen G, Shefer S, Nguyen L, Ness GC, Tint GS, Shore V. Sitosterolemia. Journal of lipid research. 33(7) : 945-55, 1992.
  • Chambers CM, McLean MP, Ness GC. Smith-Lemli-Opitz syndrome produced in rats with AY 9944 treated by intravenous injection of lipoprotein cholesterol. American journal of medical genetics. 68(3) : 322-7, 1997.
  • Nepokroeff CM, Lakshmanan MR, Ness GC, Muesing RA, Kleinsek DA, Porter JW. Coordinate control of rat liver lipogenic enzymes by insulin. Archives of biochemistry and biophysics. 162(2) : 340-4, 1974.
  • Chambers CM, Ness GC. Translational regulation of hepatic HMG-CoA reductase by dietary cholesterol. Biochemical and biophysical research communications. 232(2) : 278-81, 1997.
  • Xu G, Salen G, Batta AK, Shefer S, Nguyen LB, Niemann W, Chen TS, Arora-Mirchandani R, Ness GC, Tint GS. Glycocholic acid and glycodeoxycholic acid but not glycoursocholic acid inhibit bile acid synthesis in the rabbit. Gastroenterology. 102(5) : 1717-23, 1992.
  • Salen G, Shefer S, Nguyen L, Ness GC, Tint GS, Batta AK. Sitosterolemia. Sub-cellular biochemistry. 28: 453-76, 1997.
  • Nguyen LB, Cobb M, Shefer S, Salen G, Ness GC, Tint GS. Regulation of cholesterol biosynthesis in sitosterolemia: effects of lovastatin, cholestyramine, and dietary sterol restriction. Journal of lipid research. 32(12) : 1941-8, 1991.
  • Ness GC, Lopez D, Borrego O, Gilbert-Barness E. Increased expression of low-density lipoprotein receptors in a Smith-Lemli-Opitz infant with elevated bilirubin levels. American journal of medical genetics. 68(3) : 294-9, 1997.
  • Nepokroeff CM, Lakshmanan MR, Ness GC, Dugan RE, Porter JW. Regulation of the diurnal rhythm of rat liver beta-hydroxy-beta-methylglutaryl coenzmye A reductase activity by insulin, glucagon, cyclic AMP and hydrocortisone. Archives of biochemistry and biophysics. 160(2) : 387-96, 1974.
  • Ness GC, Pendleton LC. Thyroid hormone increases glyceraldehyde 3-phosphate dehydrogenase gene expression in rat liver. FEBS letters. 288(1-2) : 21-2, 1991.
  • Lopez D, Ness GC. Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase unmask transcriptional regulation of hepatic low-density lipoprotein receptor gene expression by dietary cholesterol. Archives of biochemistry and biophysics. 344(1) : 215-9, 1997.
  • Ness GC, Keller RK, Pendleton LC. Feedback regulation of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol is not due to altered mRNA levels. The Journal of biological chemistry. 266(23) : 14854-7, 1991.
  • Ness GC, Dugan RE, Lakshmanan MR, Nepokroeff CM, Porter JW. Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in hypophysectomized rats by L-triiodothyronine. Proceedings of the National Academy of Sciences of the United States of America. 70(12) : 3839-42, 1973.
  • Lopez D, Chambers CM, Ness GC. 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors unmask cryptic regulatory mechanisms. Archives of biochemistry and biophysics. 343(1) : 118-22, 1997.
  • Ness GC. Thyroid hormone. Basis for its hypocholesterolemic effect. The Journal of the Florida Medical Association. 78(6) : 383-5, 1991.
  • Ness GC, Chambers CM. The diurnal variation of hepatic HMG-CoA reductase activity is due to changes in the level of immunoreactive protein. Archives of biochemistry and biophysics. 327(1) : 41-4, 1996.
  • Lakshmanan MR, Nepokroeff CM, Ness GC, Dugan RE, Porter JW. Stimulation by insulin of rat liver -hydroxy- -methylglutaryl coenzyme A reductase and cholesterol-synthesizing activities. Biochemical and biophysical research communications. 50(3) : 704-10, 1973.
  • Keller RK, Zhao Z, Chambers C, Ness GC. Farnesol is not the nonsterol regulator mediating degradation of HMG-CoA reductase in rat liver. Archives of biochemistry and biophysics. 328(2) : 324-30, 1996.
  • Slakey LL, Ness GC, Qureshi N, Porter JW. Occurrence of the enzymes effecting the conversion of acetyl CoA to squalene in homogenates of hog aorta. Journal of lipid research. 14(4) : 485-94, 1973.
  • Ness GC, Zhao Z, Lopez D. Inhibitors of cholesterol biosynthesis increase hepatic low-density lipoprotein receptor protein degradation. Archives of biochemistry and biophysics. 325(2) : 242-8, 1996.
  • Xu G, Salen G, Shefer S, Ness GC, Nguyen LB, Tint GS, Parker TS, Roberts J, Batta AK, Chen TS, Zhao Z, Kong X. Increasing hepatic cholesterol 7alpha-hydroxylase reduces plasma cholesterol concentrations in normocholesterolemic and hypercholesterolemic rabbits. Hepatology (Baltimore, Md.). 24(4) : 882-7, 1996.
  • Ness GC, Takahashi T, Lee YP. Thyroid hormones on amino acid and protein metabolism. I. Concentration and composition of free amino acids in blood plasma of the rat. Endocrinology. 85(6) : 1166-71, 1969.
  • Shefer S, Nguyen LB, Salen G, Ness GC, Tint GS, Batta AK, Hauser S, Rani I. Regulation of cholesterol 7 alpha-hydroxylase by hepatic 7 alpha-hydroxylated bile acid flux and newly synthesized cholesterol supply. The Journal of biological chemistry. 266(5) : 2693-6, 1991.
  • Xu G, Salen G, Shefer S, Ness GC, Chen TS, Zhao Z, Tint GS. Reproducing abnormal cholesterol biosynthesis as seen in the Smith-Lemli-Opitz syndrome by inhibiting the conversion of 7-dehydrocholesterol to cholesterol in rats. The Journal of clinical investigation. 95(1) : 76-81, 1995.
  • Ness GC, Pendleton LC, Li YC, Chiang JY. Effect of thyroid hormone on hepatic cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase and apolipoprotein A-I mRNA levels in hypophysectomized rats. Biochemical and biophysical research communications. 172(3) : 1150-6, 1990.
  • Xu G, Salen G, Shefer S, Ness GC, Nguyen LB, Parker TS, Chen TS, Zhao Z, Donnelly TM, Tint GS. Unexpected inhibition of cholesterol 7 alpha-hydroxylase by cholesterol in New Zealand white and Watanabe heritable hyperlipidemic rabbits. The Journal of clinical investigation. 95(4) : 1497-504, 1995.
  • Xu G, Salen G, Shefer S, Ness GC, Chen TS, Zhao Z, Salen L, Tint GS. Treatment of the cholesterol biosynthetic defect in Smith-Lemli-Opitz syndrome reproduced in rats by BM 15.766. Gastroenterology. 109(4) : 1301-7, 1995.
  • Ness GC, Lopez D. Transcriptional regulation of rat hepatic low-density lipoprotein receptor and cholesterol 7 alpha hydroxylase by thyroid hormone. Archives of biochemistry and biophysics. 323(2) : 404-8, 1995.
  • Nguyen LB, Shefer S, Salen G, Ness GC, Tint GS, Zaki FG, Rani I. A molecular defect in hepatic cholesterol biosynthesis in sitosterolemia with xanthomatosis. The Journal of clinical investigation. 86(3) : 923-31, 1990.
  • Ness GC, Pendelton LC, Zhao Z. Thyroid hormone rapidly increases cholesterol 7 alpha-hydroxylase mRNA levels in hypophysectomized rats. Biochimica et biophysica acta. 1214(3) : 229-33, 1994.
  • Salen G, Batta AK, Tint GS, Shefer S, Ness GC. Inverse relationship between plasma cholestanol concentrations and bile acid synthesis in sitosterolemia. Journal of lipid research. 35(10) : 1878-87, 1994.
  • Walker RF, Ness GC, Zhao Z, Bercu BB. Effects of stimulated growth hormone secretion on age-related changes in plasma cholesterol and hepatic low density lipoprotein messenger RNA concentrations. Mechanisms of ageing and development. 75(3) : 215-26, 1994.
  • Shefer S, Salen G, Bullock J, Nguyen LB, Ness GC, Vhao Z, Belamarich PF, Chowdhary I, Lerner S, Batta AK. The effect of increased hepatic sitosterol on the regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase and cholesterol 7 alpha-hydroxylase in the rat and sitosterolemic homozygotes. Hepatology (Baltimore, Md.). 20(1 Pt 1) : 213-9, 1994.
  • Ness GC, Zhao Z, Keller RK. Effect of squalene synthase inhibition on the expression of hepatic cholesterol biosynthetic enzymes, LDL receptor, and cholesterol 7 alpha hydroxylase. Archives of biochemistry and biophysics. 311(2) : 277-85, 1994.
  • Nguyen LB, Shefer S, Salen G, Ness GC, Batta A, Chowdhary IR, Paroulek E, Hauser S. Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in the rat ileum: effects of bile acids and lovastatin. Metabolism: clinical and experimental. 43(11) : 1446-50, 1994.
  • Ness GC, Wiggins L, Zhao Z. Insulin increases hepatic 3-hydroxy-methylglutaryl coenzyme A reductase mRNA and immunoreactive protein levels in diabetic rats. Archives of biochemistry and biophysics. 309(1) : 193-4, 1994.
  • Ness GC, Eales S, Lopez D, Zhao Z. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by sterols and nonsterols in rat liver. Archives of biochemistry and biophysics. 308(2) : 420-5, 1994.
  • Ness GC, Zhao Z. Thyroid hormone rapidly induces hepatic LDL receptor mRNA levels in hypophysectomized rats. Archives of biochemistry and biophysics. 315(1) : 199-202, 1994.

Positions Held

  • Professor (Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1986 - 2006)
  • Member (Institute for Biomolecular Science, University of South Florida 1986 - 2002)
  • Associate Professor (Department of Biochemistry, College of Medicine, University of South Florida 1980 - 1986)
  • Assistant Professor (Department of Biochemistry, College of Medicine, University of South Florida 1974 - 1980)