Molecular Medicine

Xingmin Sun D-9360-2016

Xingmin Sun, PhD

Associate Professor, Department of Molecular Medicine, College of Medicine

Contact Info 12901 Bruce B. Downs Blvd
MDC07
Tampa FL 33612

Academic Email: sun5@usf.edu

Academic Phone:(813) 974-4553

View My C.V.

Education

  • PhD, Natural Sciences, Christian-Albrechts-University of Kiel, 2002
  • MS, Veterinary Microbiology & Immunology, Nanjing Agricultural University, 1994

Interdisciplinary and Emerging Signature Programs

  • Allergy, Immunology & Infectious Disease

Research Interests

  • The Sun Lab (http://www.sunlab-usf.com) focuses on the pathogenesis of Clostridium difficile infection (CDI) and development of novel preventive and therapeutic agents against CDI. We use multidisciplinary approaches in our research, including molecular biology/microbiology, immunology, cell biology, protein engineering, genome sequencing and bioinformatics, flow cytometry and nano-particle delivery. Current projects: 1) The roles of innate immunity and intestinal microbiome in the pathogenesis of CDI; 2) Physiology, virulence and pathogenesis mechanisms of C. difficile; 3) Development of novel therapeutic /preventive agents including vaccines and antimicrobials against C. difficile; 4) Antibiotic resistance mechanisms of C. difficile.

Memberships

  • Anaerobe Society of the Americas (Member, 2016 - Present)
  • Society for Mucosal Immunology (Member, 2014 - Present)
  • American Society for Microbiology (Member, 2005 - Present)

Recent Publications

  • Daou N, Wang Y, Levdikov VM, Nandakumar M, Livny J, Bouillaut L, Blagova E, Zhang K, Belitsky BR, Rhee K, Wilkinson AJ, Sun X, Sonenshein AL. Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027. PloS one. 14(1) : e0206896, 2019. http://www.ncbi.nlm.nih.gov/pubmed/30699117
  • Zhu D, Bullock J, He Y, Sun X. Cwp22, a novel peptidoglycan cross-linking enzyme, plays pleiotropic roles in Clostridioides difficile. Environmental microbiology. 21(8) : 3076-3090, 2019. http://www.ncbi.nlm.nih.gov/pubmed/31173438
  • Li C, Teng P, Peng Z, Sang P, Sun X, Cai J. Bis-Cyclic Guanidines as a Novel Class of Compounds Potent against Clostridium difficile. ChemMedChem. 13(14) : 1414-1420, 2018. http://www.ncbi.nlm.nih.gov/pubmed/29768720
  • Xu D, Han L, Li C, Cao Q, Zhu D, Barrett NH, Harmody D, Chen J, Zhu H, McCarthy PJ, Sun X, Wang G. Bioprospecting Deep-Sea Actinobacteria for Novel Anti-infective Natural Products. Frontiers in microbiology. 9: 787, 2018. http://www.ncbi.nlm.nih.gov/pubmed/29760684
  • Zhu D, Sorg JA, Sun X. Biology: Sporulation, Germination, and Corresponding Therapies for Infection. Frontiers in cellular and infection microbiology. 8: 29, 2018. http://www.ncbi.nlm.nih.gov/pubmed/29473021
  • Wang Y, Wang S, Kelly CP, Feng H, Greenberg A, Sun X. TPL2 Is a Key Regulator of Intestinal Inflammation in Clostridium difficile Infection. Infection and immunity. 86(8) , 2018. http://www.ncbi.nlm.nih.gov/pubmed/29844241
  • Teng P, Li C, Peng Z, Anne Marie V, Nimmagadda A, Su M, Li Y, Sun X, Cai J. Facilely accessible quinoline derivatives as potent antibacterial agents. Bioorganic & medicinal chemistry. 26(12) : 3573-3579, 2018. http://www.ncbi.nlm.nih.gov/pubmed/29858158
  • Wang Y, Wang S, Bouillaut L, Li C, Duan Z, Zhang K, Ju X, Tzipori S, Sonenshein AL, Sun X. Oral Immunization with Nontoxigenic Clostridium difficile Strains Expressing Chimeric Fragments of TcdA and TcdB Elicits Protective Immunity against C. difficile Infection in Both Mice and Hamsters. Infection and immunity. 86(11) , 2018. http://www.ncbi.nlm.nih.gov/pubmed/30150259
  • Wang S, Wang Y, Cai Y, Kelly CP, Sun X. Novel Chimeric Protein Vaccines Against Infection. Frontiers in immunology. 9: 2440, 2018. http://www.ncbi.nlm.nih.gov/pubmed/30405630
  • Li C, Harmanus C, Zhu D, Meng X, Wang S, Duan J, Liu S, Fu C, Zhou P, Liu R, Wu A, Kuijper EJ, Smits WK, Fu L, Sun X. Characterization of the virulence of a non-RT027, non-RT078 and binary toxin-positive Clostridium difficile strain associated with severe diarrhea. Emerging microbes & infections. 7(1) : 211, 2018. http://www.ncbi.nlm.nih.gov/pubmed/30542069
  • Peng Z, Wang S, Gide M, Zhu D, Lamabadu Warnakulasuriya Patabendige HM, Li C, Cai J, Sun X. A Novel Bacteriophage Lysin-Human Defensin Fusion Protein Is Effective in Treatment of Infection in Mice. Frontiers in microbiology. 9: 3234, 2018. http://www.ncbi.nlm.nih.gov/pubmed/30687250
  • Peng Z, Jin D, Kim HB, Stratton CW, Wu B, Tang YW, Sun X. Update on Antimicrobial Resistance in Clostridium difficile: Resistance Mechanisms and Antimicrobial Susceptibility Testing. Journal of clinical microbiology. 55(7) : 1998-2008, 2017. http://www.ncbi.nlm.nih.gov/pubmed/28404671
  • Ghose C, Eugenis I, Edwards AN, Sun X, McBride SM, Ho DD. Immunogenicity and protective efficacy of Clostridium difficile spore proteins. Anaerobe. 37: 85-95, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26688279
  • Ghose C, Eugenis I, Sun X, Edwards AN, McBride SM, Pride DT, Kelly CP, Ho DD. Immunogenicity and protective efficacy of recombinant Clostridium difficile flagellar protein FliC. Emerging Microbes & Infections. 5: e8, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26839147
  • Kim HB, Wang Y, Sun X. A Detrimental Role of Immunosuppressive Drug, Dexamethasone, During Clostridium difficile Infection in Association with a Gastrointestinal Microbial Shift. Journal of Microbiology and Biotechnology. 26(3) : 567-71, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26809802
  • Schmidt DJ, Beamer G, Tremblay JM, Steele JA, Kim HB, Wang Y, Debatis M, Sun X, Kashentseva EA, Dmitriev IP, Curiel DT, Shoemaker CB, Tzipori S. A Tetraspecific VHH-based Neutralizing Antibody Modifies Disease Outcome in Three Animal Models of Clostridium difficile Infection. Clinical and Vaccine Immunology : CVI. , 2016. http://www.ncbi.nlm.nih.gov/pubmed/27413067
  • Sponseller JK, Steele JA, Schmidt DJ, Kim HB, Beamer G, Sun X, Tzipori S. Hyperimmune bovine colostrum as a novel therapy to combat Clostridium difficile infection. The Journal of Infectious Diseases. 211(8) : 1334-41, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25381448
  • Wang YK, Yan YX, Kim HB, Ju X, Zhao S, Zhang K, Tzipori S, Sun X. A chimeric protein comprising the glucosyltransferase and cysteine proteinase domains of toxin B and the receptor binding domain of toxin A induces protective immunity against Clostridium difficile infection in mice and hamsters. Human Vaccines & Immunotherapeutics. 11(9) : 2215-22, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26036797
  • Zhang K, Zhao S, Wang Y, Zhu X, Shen H, Chen Y, Sun X. The non-toxigenic Clostridium difficile CD37 protects mice against infection with a BI/NAP1/027 type of C. difficile strain. Anaerobe. 36: 49-52, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26461425
  • Sun X, Hirota SA. The roles of host and pathogen factors and the innate immune response in the pathogenesis of Clostridium difficile infection. Molecular Immunology. 63(2) : 193-202, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25242213
  • Yong Y, Liu S, Hua G, Jia R, Zhao Y, Sun X, Liao M, Ju X. Identification and functional characterization of Toll-like receptor 2-1 in geese. BMC Veterinary Research. 11: 108, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25967535
  • Huang T, Perez-Cordon G, Shi L, Li G, Sun X, Wang X, Wang J, Feng H. Clostridium difficile toxin B intoxicated mouse colonic epithelial CT26 cells stimulate the activation of dendritic cells. Pathogens and Disease. 73(3) , 2015. http://www.ncbi.nlm.nih.gov/pubmed/25743476
  • Wang YK, Zou Q, Sun JH, Wang HA, Sun X, Chen ZF, Yan YX. Screening of single-stranded DNA (ssDNA) aptamers against a zearalenone monoclonal antibody and development of a ssDNA-based enzyme-linked oligonucleotide assay for determination of zearalenone in corn. Journal of Agricultural and Food Chemistry. 63(1) : 136-41, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25485848
  • Zhao S, Ghose-Paul C, Zhang K, Tzipori S, Sun X. Immune-based treatment and prevention of Clostridium difficile infection. Human Vaccines & Immunotherapeutics. 10(12) : 3522-30, 2014. http://www.ncbi.nlm.nih.gov/pubmed/25668664
  • Kim H, B, Q, Zhang X, Sun G, Beamer D, Schmidt Y, Wang & S, Tzipori. Effect of oral tigecycline treatment on Clostridium difficile and human gut microflora. Antimicrob Agents Chemother.. 58(12) : 7560-4, 2014.
  • Kim HB, Zhang Q, Sun X, Beamer G, Wang Y, Tzipori S. Beneficial effect of oral tigecycline treatment on Clostridium difficile infection in gnotobiotic piglets. Antimicrobial Agents and Chemotherapy. 58(12) : 7560-4, 2014. http://www.ncbi.nlm.nih.gov/pubmed/25267665
  • Ali Y, Koberg S, Heßner S, Sun X, Rabe B, Back A, Neve H, Heller KJ. Temperate Streptococcus thermophilus phages expressing superinfection exclusion proteins of the Ltp type. Frontiers in Microbiology. 5: 98, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24659988
  • Steele J, Chen K, Sun X, Zhang Y, Wang H, Tzipori S, Feng H. Systemic dissemination of Clostridium difficile toxins A and B is associated with severe, fatal disease in animal models. The Journal of Infectious Diseases. 205(3) : 384-91, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22147798
  • Steele J, K, Chen X, Sun Y, Zhang H, Wang S, Tzipori & H, Feng. Toxemia is the cause of systemic disease in the piglet and mouse models of Clostridium difficile infection. J. Infect. Dis.. 205(3) : 384-91, 2012.
  • Wang H, Sun X, Zhang Y, Li S, Chen K, Shi L, Nie W, Kumar R, Tzipori S, Wang J, Savidge T, Feng H. A chimeric toxin vaccine protects against primary and recurrent Clostridium difficile infection. Infection and Immunity. 80(8) : 2678-88, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22615245
  • Wu J, Lu Z, Nie M, Zhou H, Sun X, Xue X, Bi J, Fang G. Optimization of cryopreservation procedures for porcine endothelial progenitor cells. Journal of Bioscience and Bioengineering. 113(1) : 117-23, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22036230
  • Sun X, Wang H, Zhang Y, Chen K, Davis B, Feng H. Mouse relapse model of Clostridium difficile infection. Infection and Immunity. 79(7) : 2856-64, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21576341
  • Sun X, Savidge T, Feng H. The enterotoxicity of Clostridium difficile toxins. Toxins. 2(7) : 1848-80, 2010. http://www.ncbi.nlm.nih.gov/pubmed/22069662
  • He X, Sun X, Wang J, Wang X, Zhang Q, Tzipori S, Feng H. Antibody-enhanced, Fc gamma receptor-mediated endocytosis of Clostridium difficile toxin A. Infection and Immunity. 77(6) : 2294-303, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19307220
  • Sun X, He X, Tzipori S, Gerhard R, Feng H. Essential role of the glucosyltransferase activity in Clostridium difficile toxin-induced secretion of TNF-alpha by macrophages. Microbial Pathogenesis. 46(6) : 298-305, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19324080
  • He X, Wang J, Steele J, Sun X, Nie W, Tzipori S, Feng H. An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins. Journal of Microbiological Methods. 78(1) : 97-100, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19393695
  • Yang G, Zhou B, Wang J, He X, Sun X, Nie W, Tzipori S, Feng H. Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium. BMC Microbiology. 8: 192, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18990232
  • Sun X, Mierke DF, Biswas T, Lee SY, Landy A, Radman-Livaja M. Architecture of the 99 bp DNA-six-protein regulatory complex of the lambda att site. Molecular Cell. 24(4) : 569-80, 2006. http://www.ncbi.nlm.nih.gov/pubmed/17114059
  • Sun X, Göhler A, Heller KJ, Neve H. The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis. Virology. 350(1) : 146-57, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16643978

Positions Held

  • Assistant Professor (Department of Molecular Medicine, College of Medicine, University of South Florida 2015 - 2019)
  • Assistant Professor (adjunct appointment) (Clinical and Translational Science Institute, Tufts University 2014 - 2015)
  • Research Assistant Professor (Department of Infectious Disease and Global Health, Tufts University Cummings School of Veterinary Medicine 2012 - 2015)

Awards/Honors

  • Excellence in Innovation Award (University of South Florida - 2020)
  • Faculty Outstanding Research Achievement Award (University of South Florida - 2018)
  • Tufts Institute for Innovation Inaugural Award (Tufts University - 2014)
  • K01 Mentored Research Scientist Development Award (NIH/NIDDK - 2012)
  • ESCMID (European Society of Clinical Microbiology and Infectious Diseases) Award for the "6th (European Society of Clinical Microbiology and Infectious Diseases - 2009)
  • Fellow for 48th Annual ICAAC/46th IDSA (American Society for Microbiology and the Infectious Diseases Society of America - 2008)
  • Postdoctoral Travel Award (American Society for Biochemistry and Molecular Biology - 2007)
  • Young Scientist Award (Federation of European Microbiological Societies for the 7th Symposium on Lactic - 2002)
  • Excellent Graduate Student Award (Nanjing Agricultural University - 1993)
  • Excellent Graduate Student Award (Nanjing Agricultural University - 1992)