Proteomics
Services Offered
The proteomics core offers a range of techniques for the identification, characterization, and quantification of proteins and peptides.
Protein and Peptide Separation
- High pH (pH 10) reverse phase chromatography, for offline fraction collection
- SDS-PAGE gel electrophoresis.
Protein Digestion and Labeling
- Protein digestion in-gel or in-solution
- Peptide purification using Zip-tips (Millipore), Desalting columns (Peirce), Hypersep (Thermo)
- Peptide labeling with stable isotopes for relative protein quantification
Mass Spectrometry: MS, LC-MS and LC-MS/MS
- Complex proteome analysis (discovery)
- Parallel reaction monitoring (PRM) for targeted identification of proteins/ PTMs
- Relative quantification of proteins by stable isotope labeling (SILAC, iTraq)
- Identification of phosphorylation and other post-translational modification
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ACADEMIC |
ACADEMIC |
Non-USF ACADEMIC |
INDUSTRY |
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Service |
Gradient/Instrument Time |
Assisted* |
Unassisted** |
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Proteome Characterization from Complex Sample |
3 hours |
$95.00 |
$70.00 |
$150.00 |
$190.00 |
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2 hours |
$90.00 |
$65.00 |
$140.00 |
$180.00 | |
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1 hour |
$85.00 |
$60.00 |
$135.00 |
$170.00 | |
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Proteome Characterization with gel digest |
1 hour |
$100.00 |
$80.00 |
$160.00 |
$200.00 |
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PRM/targeted analysis Initial workup (includes test runs and method creation) |
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$150.00 |
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$250.00 |
$300.00 |
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Protein Assay (660nm) |
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$9.00 |
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$12.00 |
$18.00 |
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Protein assay (600nm+IDCR) |
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$12.00 |
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$18.00 |
$24.00 |