The proteomics core offers a range of techniques for the identification, characterization, and quantification of proteins and peptides.

Protein and Peptide Separation

• High pH (pH 10) reverse phase chromatography, for offline fraction collection
• SDS-PAGE gel electrophoresis.

Protein Digestion and Labeling

• Protein digestion in-gel or in-solution
• Peptide purification using Zip-tips (Millipore), Desalting columns (Peirce), Hypersep (Thermo)
• Peptide labeling with stable isotopes for relative protein quantification

Mass Spectrometry: MS, LC-MS and LC-MS/MS

• Complex proteome analysis (discovery)
• Parallel reaction monitoring (PRM) for targeted identification of proteins/ PTMs
• Relative quantification of proteins by stable isotope labeling (SILAC, iTraq)
• Identification of phosphorylation and other post-translational modification

		ACADEMIC	ACADEMIC	Non-USF ACADEMIC	INDUSTRY
Service	Gradient/Instrument Time	Assisted*	Unassisted**
Proteome Characterization from Complex Sample	3 hours	$95.00	$70.00	$150.00	$190.00
	2 hours	$90.00	$65.00	$140.00	$180.00
	1 hour	$85.00	$60.00	$135.00	$170.00
Proteome Characterization with gel digest	1 hour	$100.00	$80.00	$160.00	$200.00
PRM/targeted analysis Initial workup (includes test runs and method creation)		$150.00		$250.00	$300.00
Protein Assay (660nm)		$9.00		$12.00	$18.00
Protein assay (600nm+IDCR)		$12.00		$18.00	$24.00