Proteomics

Services Offered

The proteomics core offers a range of techniques for the identification, characterization, and quantification of proteins and peptides.

Protein and Peptide Separation

  • High pH (pH 10) reverse phase chromatography, for offline fraction collection
  • SDS-PAGE gel electrophoresis.

Protein Digestion and Labeling

  • Protein digestion in-gel or in-solution
  • Peptide purification using Zip-tips (Millipore), Desalting columns (Peirce), Hypersep (Thermo)
  • Peptide labeling with stable isotopes for relative protein quantification

Mass Spectrometry: MS, LC-MS and LC-MS/MS

  • Complex proteome analysis (discovery)
  • Parallel reaction monitoring (PRM) for targeted identification of proteins/ PTMs
  • Relative quantification of proteins by stable isotope labeling (SILAC, iTraq)
  • Identification of phosphorylation and other post-translational modification

 

 

ACADEMIC

ACADEMIC

non-ACADEMIC

Service

Gradient/Instrument Time

Assisted*

Unassisted**

 

Proteome Characterization from Complex Sample (filter digest)

3 hours

$90.00

$70.00

$180.00

2 hours

$85.00

$65.00

$170.00

1 hour

$80.00

$60.00

$160.00

Proteome Characterization from Complex Sample (in gel digest)

1 hour

$100.00

$80.00

$200.00

Purified protein or IP with filter digest

1 hour

$85.00

$65.00

$170.00

Purified protein or IP with in-gel digest

1 hour

$89.00

$70.00

$178.00

PRM/targeted analysis Initial workup (includes test runs and method creation)

 

$150.00

 

$300.00

Protein Assay (660nm)

 

$9.00

 

$18.00

Protein assay (600nm+IDCR)

 

$12.00

 

$24.00