Dr. White is a professor in the College of Public Health as well as the deputy director for the Center for Drug Discovery and Innovation. His lab focuses on how the malaria-related protozoa, Toxoplasma gondii, grows and develops in its human host. Employing functional genomic methods, he has defined the cell cycle transcriptome of these parasites and is studying a unique set of plant-like transcription factors, called ApiAP2 proteins, that
are responsible for controlling parasite development in the human host. The White lab uses forward genetic approaches to identify key factors responsible for regulating the complex cell cycle of these parasites. Whole genome
sequencing of a large collection of parasite mutants, has led to the discovery of novel factors that are essential for parasite replication.
Radke, J.B., Worth, D., Hong, D.,
Huang, S., Sullivan Jr, W.J., Wilson, E.H., White, M.W. (2018) Transcriptional
repression by ApiAP2 factors is central to chronic toxoplasmosis. PLoS
Pathogens May 2;14(5):e1007035. doi: 10.1371/journal.ppat.1007035 PMID:29718996
White, M.W. and Suvorova, E. (2018)
Apicomplexa development: something old, borrowed, lost and new. Trends in
Parasitology Available at [https://doi.org/10.1016/j.pt.2018.07.006].
Hong, D., Radke, J. B., White, M.W.
(2017) Opposing transcriptional mechanisms regulate Toxoplasma development.
Preprint available doi:https://doi.org/10.1101/094847. Also available mSphere
Feb 2017, 2 (1) e00347-16. doi: 10.1128/mSphere.00347-16 PMID:28251183
Huang, S., Holmes, M.J., Radke,
J.B., Hong, D., Liu, T., White, M.W., Sullivan Jr., W.J. (2017) Toxoplasma
gondii AP2IX-4 regulates gene expression during bradyzoite development.
Preprint available: doi: https://doi.org/10.1101/104208. Also available mSphere,
Mar 15;2(2) DOI: 10.1128/mSphere.00054-17. PMID:28317026
Naumov, A., Kratzer, S., Chen, L.
M., Suvorova, E. S., Kim, K., White, M. W., (2017) The Toxoplasma centrocone
houses cell cycle regulatory factors. Preprint available, doi:
https://doi.org/10.1101/122465. Also available in mBio 8:e00579-17. https://doi.org/10.1128/mBio.00579-17
Lorenzi H, Khan A, Behnke MS, Namasivayam S, Swapna LS, Hadjithomas M, Karamycheva S, Pinney D, Brunk BP, Ajioka JW, Ajzenberg D, Boothroyd JC, Boyle JP, Dardé ML, Diaz-Miranda MA, Dubey JP, Fritz HM, Gennari SM, Gregory BD, Kim K, Saeij JPJ, Su C, White MW, Zhu X-Q, Howe DK, Rosenthal BM, Grigg ME, Parkinson J, Liu L, Kissinger JC, Roos DS, Sibley LD. 2016. Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nat Commun 7:10147.
El Bissati K, Suvorova ES, Xiao H, Lucas O, Upadhya R, Ma Y, Angeletti RH, White MW, Weiss LM, Kim K. 2016. Toxoplasma gondii Arginine Methyltransferase 1 (PRMT1) Is Necessary for Centrosome Dynamics during Tachyzoite Cell Division. MBio 7:e02094-15.
Engelberg K, Ivey FD, Lin A, Kono M, Lorestani A, Faugno-Fusci D, Gilberger T-W, White M, Gubbels M-J. 2016. A MORN1-associated HAD phosphatase in the basal complex is essential for Toxoplasma gondii daughter budding. Cell Microbiol 18:1153–71.
Sugi T, Ma YF, Tomita T, Murakoshi F, Eaton MS, Yakubu R, Han B, Tu V, Kato K, Kawazu S-I, Gupta N, Suvorova ES, White MW, Kim K, Weiss LM. 2016. Toxoplasma gondii Cyclic AMP-Dependent Protein Kinase Subunit 3 Is Involved in the Switch from Tachyzoite to Bradyzoite Development. MBio 7.
Suvorova ES, Francia M, Striepen B, White MW. 2015. A novel bipartite centrosome coordinates the apicomplexan cell cycle. PLoS Biol 13:e1002093.
Vinayak S, Brooks CF, Naumov A, Suvorova ES, White MW, Striepen B. 2014. Genetic manipulation of the Toxoplasma gondii genome by fosmid recombineering. MBio 5:e02021.
Brown KM, Suvorova E, Farrell A, McLain A, Dittmar A, Wiley GB, Marth G, Gaffney PM, Gubbels MJ, White M, Blader IJ. 2014. Forward genetic screening identifies a small molecule that blocks Toxoplasma gondii growth by inhibiting both host- and parasite-encoded kinases. PLoS Pathog 10:e1004180.
Croken MM, Qiu W, White MW, Kim K. 2014. Gene Set Enrichment Analysis (GSEA) of Toxoplasma gondii expression datasets links cell cycle progression and the bradyzoite developmental program. BMC Genomics 15:515.
Suvorova ES, Croken M, Kratzer S, Ting L-M, Conde de Felipe M, Balu B, Markillie ML, Weiss LM, Kim K, White MW. 2013. Discovery of a splicing regulator required for cell cycle progression. PLoS Genet 9:e1003305.
Radke JB, Lucas O, De Silva EK, Ma Y, Sullivan WJ, Weiss LM, Llinas M, White MW. 2013. ApiAP2 transcription factor restricts development of the Toxoplasma tissue cyst. Proc Natl Acad Sci U S A 110:6871–6.
Suvorova ES, Radke JB, Ting L-M, Vinayak S, Alvarez CA, Kratzer S, Kim K, Striepen B, White MW. 2013. A nucleolar AAA-NTPase is required for parasite division. Mol Microbiol 90:338–55.
Szatanek T, Anderson-White BR, Faugno-Fusci DM, White M, Saeij JPJ, Gubbels M-J. 2012. Cactin is essential for G1 progression in Toxoplasma gondii. Mol Microbiol 84:566–77.