The USF Genomics Program offers two types of training workshops, the RNA-seq Illumina Sequencing Laboratory Workshop and the RNA-seq Data Analysis Computational Workshop. Each workshop is offered three times a year. These workshops are open to all in the USF community and required for access to use the USF Genomics shared equipment.
This training course includes presentations on technology theory and practical experience with entire laboratory sample prep workflow. Experienced trainers will lead you through using TapeStation and Miseq equipment. By the end of this training, you will be able to: complete Illumina library preparation process and set up a sequencing run using Illumina Miseq Control Software.
This 5-day in-depth course will instruct participants on the theoretical and practical concepts related to RNA sequencing analysis, enabling them to perform these analyses independently.
The first workshop module is an introduction to data analysis using Linux, assuring that all participants are able to follow the practical parts. The second module discusses advantages and disadvantages of current sequencing technologies and their implications on data analysis. The third module introduces preprocessing raw reads, read mapping, visualization of mapped reads and one proceeds with first hands-on analyses (QC, mapping, visualization). The fourth module introduces ‘R’ a programming language to perform statistical computing and visualization, and ‘Bioconductor’ an R based toolshed for analyzing genomic data. The last module addresses quantification of gene expression and predict differentially expressed genes, followed by a whole day hands-on RNA-Seq analysis pipeline: TUXEDO protocol.
Day 1 (Friday): Illumina Sequencing Introduction & Computer and Software Check (open to non-registered participants)
Day 2 (Friday): Illumina Sequencing Refresher & Linux for Bioinformatics
Day 3 (Monday): Introduction to RNA sequencing & RNA-seq Alignment and Visualization
Day 4 (Tuesday): Introduction to R and Bioconductor & Expression and Differential Expression
Day 5 (Wednesday): RNA-seq - TUXEDO Pipeline Hands-onWorkshop Structure
If you have questions regarding the USF Genomics Workshops, email email@example.com.
Due to enrollment caps, there is a waitlist for the USF Genomics Workshops. You will be contacted after registering about the next available workshop.