The Allergy/Immunology Laboratory of the Division of Allergy & Immunology focuses on dysregulation related to primary immunodefiencies in children and adolescents.
The Division of Allergy & Immunology also has a comprehensive clinical program and the only accredited Pediatric Allergy & Immunology Fellowship Training Program in Florida.
The laboratory occupies approximately 2400 sq ft within the Children's Research Institute of the USF/All Children's Hospital located in St. Petersburg, FL. Key laboratory equipment includes: 3-laser Becton Dickinson LSR II flow cytometer, 3-laser BD FACSAria cell sorter, BD FACSCalibur flow cytometer, ABI Prism thermocycler for real-time quantitative PCR, Coulter ACT-2 diff whole blood analyzer, Molecular Devices, AquaMax 4000 ELISA plate wsher for 96- and 384-well plates and a Bio-Tek EL-800 colorimetric ELISA plate reader. The BD FACSAria sorter is housed within a 10 ft. class II BSC for sterile sorting and sorting of infectious material.
The laboratory equipment also includes a variety of +4C, -20C, and -80C refrigerators and freezers, incubators, microscopes, and centrifuges. A sample repository is included within the lab space consisting of an MVE liquid nitrogen storage facility capable of storing up to 75,000 1 ml samples. The storage facilities are monitored by a A2LA accredited Veriteq temperature monitoring system. Samples are tracked using bar-coded labels and the Laboratory Data Management System (LDMS).
The laboratory is CLIA certified and it serves as the sample processing and protocol repository for ATN, IMPAACT and participates in A/I research protocols.
Children's Research Institute
601 4th Street South, CRI 4008
Saint Petersburg, FL 33701