Andreas Seyfang H-2445-2012

Andreas Seyfang, PH.D.

Associate Professor, Department of Molecular Medicine, College of Medicine

Associate Professor (Joint Appointment), Department of Internal Medicine- Division of Infectious Diseases, College of Medicine

Associate Professor, College of Medicine Molecular Medicine

Associate Professor (Joint Appointment), Department of Neurosurgery, College of Medicine

Associate Professor (Joint Appointment), Department of Global Health, College of Public Health

Associate Professor (Joint Appointment), School of Physical Therapy & Rehabilitation Sciences, College of Medicine

Contact Info Molecular Medicine, MDC07
12901 Bruce B. Downs Blvd.
Tampa FL 33612

Academic Email: aseyfang@health.usf.edu

Academic Phone:(813) 974-2332

View My C.V.

Education

  • Postdoc, Molecular Microbiology, Oregon Health Sciences University, 1995
  • PHD, Biochemistry, University of Tubingen, 1993
  • Postdoc, Molecular Parasitology, University of Bordeaux, 1993
  • MSc, Parasitology, Zoology, University of Tübingen, 1989
  • DAAD Scholar, Microbiology, Parasitology, Duke University, 1984
  • BSc, Biology, University of Tübingen, 1983

Interdisciplinary and Emerging Signature Programs

  • Allergy, Immunology & Infectious Disease
  • Cardiovascular
  • Neuroscience

Research Interests

  • LABORATORY OF MEDICAL MICROBIOLOGY AND MOLECULAR PARASITOLOGY:
  • Research in the Seyfang laboratory focuses on two major projects (i) membrane permeases (transporters) as target for drug delivery and (ii) cytochrome b5 reductase as enzymatic drug target in opportunistic microbial pathogens including protozoan parasites and nosocomial and neuro-pathogenic fungi.
  • We are studying membrane permeases (transporters) and receptors in opportunistic microbial pathogens including protozoan parasites (trypanosomes, Leishmania) and nosocomial and neuro-pathogenic fungi (Candida albicans, Cryptococcus neoformans). We use a multi-disciplinary approach of biochemistry, proteomics, molecular pharmacology and genetics to investigate the structure-function relationship, substrate/drug selectivity, protein-protein interaction, and significance for microbial pathogenicity of these membrane proteins at the molecular level in both in vitro culture and animal models. The objective of this project is to use membrane permeases, such as the myo-inositol transporter MIT, for delivery of cytotoxic and parasite-specific substrate analogues as novel pharmacological targets in these microbial pathogens.
  • A second project investigates cytochrome b5 reductase as enzymatic drug target in these opportunistic pathogens. Cytochrome b5 reductase (Cb5r) plays an important role in P450-mediated detoxification of xenobiotics and drugs, lipid biosynthesis, and the synthesis of cholesterol (humans, Leishmania) or ergosterol (fungi and Trypanosoma cruzi). Hence we use recombinant Cb5r protein for biochemical, structural and pharmacological studies and in silico modeling as a novel pharmacological target in these opportunistic microbial pathogens.
  • TECHNIQUES that are applied in the laboratory include protein biochemistry and proteomics, microarray and real-time PCR gene expression analysis, site-directed mutagenesis, targeted gene disruption and reverse genetics, heterologeous expression in Xenopus oocytes microinjected with transporter RNA, and the generation of transgenic protozoa and candida cells. Furthermore, we use axenic cultivation of both mammalian and insect forms of trypanosomes and Leishmania, and candida (yeast and hyphae forms) to probe the pharmacology of these important permeases in vivo. Hence, our studies are aimed to develop novel inositol-based drugs such as fluoro-inositol analogues specific for these microbial pathogens.

Memberships

  • American Society for Microbiology (Member, 2008 - Present)
  • American Association for the Advancement of Science (Member, 2008 - Present)
  • Society for Neuroscience (Member, 2008 - Present)
  • American Society for Biochemistry and Molecular Biology (Member, 2008 - Present)

Recent Publications

  • Duffy AR, Beckie TM, Brenner LA, Beckstead JW, Seyfang A, Postolache TT, Groer MW. Relationship Between Toxoplasma gondii and Mood Disturbance in Women Veterans. Military Medicine. 180(6) : 621-5, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26032378
  • Seyfang, A., Saporta, S. & Johnson, W.E. Effect of Pre-Medical Education on Student Performance in a First-Year Medical School Curriculum. Medical Science Educator. 23(4) : 712, 2013.
  • Seyfang, A., Nazian, S.J., Saporta, S., Doupnik, C.A., Johnson, W.E. & Stevenson, F.T. Assessment of an Adjusted vs. Fixed Pass Line for Student Performance in a Medical School Curriculum. Medical Science Educator. 22(4) : 270-271, 2012.
  • Groër MW, Yolken RH, Xiao JC, Beckstead JW, Fuchs D, Mohapatra SS, Seyfang A, Postolache TT. Prenatal depression and anxiety in Toxoplasma gondii-positive women. American journal of obstetrics and gynecology. 204(5) : 433.e1-7, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21345406
  • Xue C, Liu T, Chen L, Li W, Liu I, Kronstad JW, Seyfang A, Heitman J. Role of an expanded inositol transporter repertoire in Cryptococcus neoformans sexual reproduction and virulence. mBio. 1(1) : e00084-10, 2010. http://www.ncbi.nlm.nih.gov/pubmed/20689743
  • Liu L, Bailey SM, Okuka M, Muñoz P, Li C, Zhou L, Wu C, Czerwiec E, Sandler L, Seyfang A, Blasco MA, Keefe DL. Telomere lengthening early in development. Nature Cell Biology. 9(12) : 1436-41, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17982445
  • Lin T, Gao L, Seyfang A, Oliver JH. 'Candidatus Borrelia texasensis', from the American dog tick Dermacentor variabilis. International Journal of Systematic and Evolutionary Microbiology. 55(Pt 2) : 685-93, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15774644
  • Seyfang A, Jin JH. Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round. Analytical Biochemistry. 324(2) : 285-91, 2004. http://www.ncbi.nlm.nih.gov/pubmed/14690693
  • Mongan TP, Ganapasam S, Hobbs SB, Seyfang A. Substrate specificity of the Leishmania donovani myo-inositol transporter: critical role of inositol C-2, C-3 and C-5 hydroxyl groups. Molecular and Biochemical Parasitology. 135(1) : 133-41, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15287594
  • Ling J, Pi W, Yu X, Bengra C, Long Q, Jin H, Seyfang A, Tuan D. The ERV-9 LTR enhancer is not blocked by the HS5 insulator and synthesizes through the HS5 site non-coding, long RNAs that regulate LTR enhancer function. Nucleic Acids Research. 31(15) : 4582-96, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12888519
  • Jin JH, Seyfang A. High-affinity myo-inositol transport in Candida albicans: substrate specificity and pharmacology. Microbiology. 149(Pt 12) : 3371-81, 2003. http://www.ncbi.nlm.nih.gov/pubmed/14663071
  • Seyfang A, Landfear SM. Four conserved cytoplasmic sequence motifs are important for transport function of the Leishmania inositol/H(+) symporter. The Journal of Biological Chemistry. 275(8) : 5687-93, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10681553
  • Seyfang A, Landfear SM. Substrate depletion upregulates uptake of myo-inositol, glucose and adenosine in Leishmania. Molecular and Biochemical Parasitology. 104(1) : 121-30, 1999. http://www.ncbi.nlm.nih.gov/pubmed/10589986
  • Barrett MP, Tetaud E, Seyfang A, Bringaud F, Baltz T. Trypanosome glucose transporters. Molecular and Biochemical Parasitology. 91(1) : 195-205, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9574935
  • Vasudevan G, Carter NS, Drew ME, Beverley SM, Sanchez MA, Seyfang A, Ullman B, Landfear SM. Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant. Proceedings of the National Academy of Sciences of the United States of America. 95(17) : 9873-8, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9707568
  • Seyfang A, Kavanaugh MP, Landfear SM. Aspartate 19 and glutamate 121 are critical for transport function of the myo-inositol/H+ symporter from Leishmania donovani. The Journal of Biological Chemistry. 272(39) : 24210-5, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9305873
  • Wille U, Seyfang A, Duszenko M. Glucose uptake occurs by facilitated diffusion in procyclic forms of Trypanosoma brucei. European Journal of Biochemistry / FEBS. 236(1) : 228-33, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8617269
  • Bakalara N, Seyfang A, Baltz T, Davis C. Trypanosoma brucei and Trypanosoma cruzi: life cycle-regulated protein tyrosine phosphatase activity. Experimental Parasitology. 81(3) : 302-12, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7498427
  • Barrett MP, Tetaud E, Seyfang A, Bringaud F, Baltz T. Functional expression and characterization of the Trypanosoma brucei procyclic glucose transporter, THT2. The Biochemical Journal. 312 ( Pt 3): 687-91, 1995. http://www.ncbi.nlm.nih.gov/pubmed/8554506
  • Bakalara N, Seyfang A, Davis C, Baltz T. Characterization of a life-cycle-stage-regulated membrane protein tyrosine phosphatase in Trypanosoma brucei. European Journal of Biochemistry / FEBS. 234(3) : 871-7, 1995. http://www.ncbi.nlm.nih.gov/pubmed/8575447
  • Seyfang A, Duszenko M. Functional reconstitution of the Trypanosoma brucei plasma-membrane D-glucose transporter. European Journal of Biochemistry / FEBS. 214(2) : 593-7, 1993. http://www.ncbi.nlm.nih.gov/pubmed/8513808
  • Duszenko, M. Seyfang, A. Endocytosis and Intracellular Transport of Variant Surface Glycoproteins in Trypanosomes. Advances in Cell and Molecular Biology of Membranes: Membrane Traffic in Protozoa. JAI Press. Greenwich(Plattner, H.; Ed.) : 227-258, 1993.
  • Seyfang A, Duszenko M. Specificity of glucose transport in Trypanosoma brucei. Effective inhibition by phloretin and cytochalasin B. European Journal of Biochemistry / FEBS. 202(1) : 191-6, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1935976
  • Seyfang A, Mecke D, Duszenko M. Degradation, recycling, and shedding of Trypanosoma brucei variant surface glycoprotein. The Journal of Protozoology. 37(6) : 546-52, 1990. http://www.ncbi.nlm.nih.gov/pubmed/2086784
  • Frommel TO, Seyfang A, Balber AE. Trypanosoma brucei sspp.: cleavage of variant specific and common glycoproteins during exposure of live cells to trypsin. Experimental Parasitology. 66(2) : 213-24, 1988. http://www.ncbi.nlm.nih.gov/pubmed/2456221

Awards/Honors

  • Outstanding Freshman Instructor Award (USF Second-year MD student class - 2019)
  • GPIBS Award for Excellence in Teaching (USF PhD and Master's graduate students - 2019)
  • Outstanding Pre-Clinical Teaching Award (USF Third-year MD student class - 2017)
  • Outstanding Freshman Instructor Award (USF Second-year MD student class - 2017)
  • Recognition of Outstanding Dedication to Teaching (USF Morsani College of Medicine - 2015)
  • Robert J. Grasso Award for Outstanding Dedication to Graduate Education (USF College of Medicine - 2009)
  • AHA Fellowship (American Heart Association - 1997)
  • Alexander von Humboldt Fellow (Alexander von Humboldt Foundation - 1995)
  • FRM Fellowship (Fondation pour la Recherche Médicale (France) - 1994)
  • EMBO Fellowship (European Molecular Biology Organization - 1993)
  • PhD, Biochemistry with Magna cum laude (University of Tübingen - 1993)
  • DAAD Fellowship (German Academic Exchange Service - 1984)