GHIDR Faculty

Wilbur Milhous, Ph.D.

Director
Associate Dean for Research
Professor
Office: CPH 1139
Email: wmilhous@health.usf.edu

  • Biography

    Professor Wilbur K. Milhous is the Associate Dean for Research and a Professor of Global Health Infectious Disease Research (GHIDR) in the USF College of Public Health. He is a South Carolina native with both a BS and MS from Clemson University. He received his PhD in 1983 from the UNC Gillings School of Global Health, in a combined training-with-industry program at the Burroughs Wellcome Fund (now GSK), and has 33 years of experience in small molecule drug development with a primary focus on malaria and emerging diseases. After UNC, Dr. Milhous joined the Walter Reed Army Institute of Research (WRAIR) working in Experimental Therapeutics for discovery, lead optimization and translational research in drug development for the Military Infectious Disease Research Program (Research Coordinator) and became the Consultant to the Army Surgeon General in Medical Microbiology and Chief Science Officer for Therapeutics at WRAIR. In 2007, Dr. Milhous joined the faculty of the USF GHIDR Center and became a member of the Medicines for Malaria Expert Scientific Advisory Committee.  He was founding co-chair of the American Society of Tropical Medicine and Hygiene (ASTMH) Committee on Global Health. He is a Fellow of ASTMH and the American Academy of Microbiology and an American Board of Medical Microbiology and Professor Emeritus Diplomat in Medical and Public Health at WRAIR. Dr. Milhous has active affiliations and leadership roles in managing the College’s diverse multimillion ($50M in 2015/16) research portfolio at USF to include the Center for Drug, Discovery, and Innovation.  He holds a joint appointment with the College of Medicine, Department of Internal Medicine.  As a 32-year member of the ASTMH, he has received numerous honors and awards to include the ASTMH-LePrince Medal in Malariology, the Gorgas Medal, ASTMH-Ashford Medal, Distinguished Alumnus of UNC, USF Research Achievement Award as well as numerous military awards during active federal service.  Professor Milhous is Editor of Antimicrobial Agents and Chemotherapy and a member of editorial boards (JCM, JTM) and a reviewer of 8 primary journals. He has over 170 publications with over 10,000 citations including 14 books/book chapters and 10 therapeutic patents. 

    For more Information:
    Dr. Milhous'Faculty Page
    https://scholar.google.com/citations?user=uH1mQ-4AAAAJ&hl=en 



Thomas Unnasch

Thomas Unnasch, PhD

Chair, Department of Global Health
Disease control and elimination of vector – borne pathogen
Office: CPH 1118, IDRB 422 (Lab)
Email:  tunnasch@health.usf.edu

 

  • Biography

    Dr. Unnasch is the Chair of the COPH Department of Global Health. He received his PhD in enzymology from the Massachusetts Institute of Technology in 1981. He joined USF in 2007 after 10 years as a professor at the University of Alabama, Birmingham. Dr. Unnasch’s lab focuses on vector-borne diseases and human filarial infections. Currently, he is working to develop new treatment options for filarial parasites by targeting the ecdysteroid signaling pathway to disrupt parasite reproduction and development. He is investigating the molecular basis of the relationship between B. malayi and its wolbachia endosymbiont with the goal of identifying new ways to disrupt this essential relationship. His lab is also involved in developing new tools to enhance the efficacy of surveillance activities carried out by onchocerciasis (river blindness) control and elimination programs in Africa and Latin America. He is working to develop improved diagnostic methods as well as mathematical algorithms to use data collected from screening pools of vectors to quantify the intensity of exposure in the affected human population. He works closely with collaborators in Africa and Latin America to develop spatial models to predict zones that are at risk of onchocerciasis using remote sensing data and to develop more efficient methods for the collection of the vector blackflies to monitor transmission.

    Additionally, he runs the Center’s Biosafety-Level 3 laboratory studying the ecology of Eastern Equine Encephalitis Virus (EEEV) and other viral encephalopathies. Specifically, he is working to elucidate the dynamic processes in the host-vector relationship that drive the development of EEEV enzootics and epidemics. The ultimate goal of these studies is to develop a predictive model for habitats that are most likely to represent EEEV enzootic foci. Such a model could be used to most efficiently target the surveillance and vector control efforts of the mosquito control programs throughout Florida and the Southeastern USA.

    Dr. Unnasch is a fellow of the American Society of Tropical Medicine and Hygiene (ASTMH), a Distinguished USF Health Professor, and State of Florida World Class Scholar. He is a member of the editorial Boards of Molecular and Biochemical Parasitology and PloS Neglected Tropical Diseases, and Editor in Chief of Research Reports in Tropical Medicine.

    More Information:
    Dr. Unnasch's faculty page
    Department of Global Health

     

  • Courses

    • Laboratory Techniques in Public Health PHC 6561
      • This is a unique interactive laboratory based course. Each lecture will be supported by a “wet lab” where students would get hands on experience of laboratory research techniques using basic and advanced biochemical and molecular tools.
    • Population Assessment II
    • Laboratory Rotations in Global Health
    • Foundations of Global Health
    • Special Topics in Global Health Infectious Disease Research
    • Independent Study: Public Health
    • Supervised Field Experience
    • Special Project: MPH
    • Directed Research
    • Dissertation
  • Current Funding

    Functional Interplay Between Brugia and its Wolbachia Symbiont
    NIH/NIAID/R56AI118936  

    Community-Directed Vector Control to Enhance Mass Drug Administration for Onchocerciasis Elimination in Africa
    NIH/NIAID/R01AI123245
  • Recent Publications

    Gervasi SS, Burkett-Cadena N, Burgan SC, Schrey AW, Hassan HK, Unnasch TR, Martin LB. 2016. Host stress hormones alter vector feeding preferences, success, and productivity. Proc Biol Sci 283.

    Bennuru S, Cotton JA, Ribeiro JM, Grote A, Harsha B, Holroyd N, et al. Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont. MBio. 2016;7(6). Epub 2016/11/25. doi: 10.1128/mBio.02028-16. PubMed PMID: 27881553.  

    Choi YJ, Tyagi R, McNulty SN, Rosa BA, Ozersky P, Martin J, et al. Genomic diversity in Onchocerca volvulus and its Wolbachia endosymbiont. Nature Microbiol. 2016;2:16207. Epub 2016/11/22. doi: 10.1038/nmicrobiol.2016.207. PubMed PMID: 27869792.  

    Cotton JA, Bennuru S, Grote A, Harsha B, Tracey A, Beech R, et al. The genome of Onchocerca volvulus, agent of river blindness. Nature Microbiol. 2016;2:16216. Epub 2016/11/22. doi: 10.1038/nmicrobiol.2016.216. PubMed PMID: 27869790.  

    Golden A, Stevens EJ, Yokobe L, Faulx D, Kalnoky M, Peck R, et al. A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus. PLoS Negl Trop Dis. 2016;10(1):e0004292. doi: 10.1371/journal.pntd.. eCollection 2016 Jan.  

    Mhashilkar AS, Vankayala SL, Liu C, Kearns F, Mehrotra P, Tzertzinis G, Palli SR, Woodcock HL, Unnasch TR. 2016. Identification of Ecdysone Hormone Receptor Agonists as a Therapeutic Approach for Treating Filarial Infections. PLoS Negl Trop Dis 10:e0004772.  

    Zarroug IM, Hashim K, ElMubark WA, Shumo ZA, Salih KA, ElNojomi NA, et al. The First Confirmed Elimination of an Onchocerciasis Focus in Africa: Abu Hamed, Sudan. Am J Trop Med Hyg. 2016;27:16-0274.  

    Voronin D, Bachu S, Shlossman M, Unnasch TR, Ghedin E, Lustigman S. 2016. Glucose and Glycogen Metabolism in Brugia malayi Is Associated with Wolbachia Symbiont Fitness. PLoS One 11:e0153812  

    Stockwell TB, Heberlein-Larson LA, Tan Y, Halpin RA, Fedorova N, Katzel DA, Smole S, Unnasch TR, Kramer LD, Das SR. 2015. First Complete Genome Sequences of Two Keystone Viruses from Florida. Genome Announc 3.  

    Young RM, Burkett-Cadena ND, McGaha TW, Rodriguez-Perez MA, Toé LD, Adeleke MA, Sanfo M, Soungalo T, Katholi CR, Noblet R, Fadamiro H, Torres-Estrada JL, Salinas-Carmona MC, Baker B, Unnasch TR, et al. 2015. Identification of Human Semiochemicals Attractive to the Major Vectors of Onchocerciasis. PLoS Negl Trop Dis 9:e3450.  

    Liu C, Voronin D, Poole CB, Bachu S, Rogers MB, Jin J, Ghedin E, Lustigman S, McReynolds LA, Unnasch TR. 2015. Functional analysis of microRNA activity in Brugia malayi. Int J Parasitol 45:579–83.  



John H. Adams, Ph.D.

John Adams, PhD

Professor, Department of Global Health
Biological investigation of the malaria parasite
Office: IDRB 304
Email: jadams3@health.usf.edu

 

  • Biography

    Dr. Adams is a distinguished Professor in the College of public Health. He received a BA from Hendrix College in Arkansas before attending the University of Illinois where he received his MSc and PhD in veterinary medical science. He spent 16 years as a professor at the University of Notre Dame before joining the faculty of the Department of Global Health in 2007. Dr. Adams’ research focuses on host-parasite interactions and improving the understanding of processes critical for infection and pathogenesis in malaria infections caused by Plasmodium falciparum and Plasmodium vivax with the expectation that an improved understanding of parasite biology will enable development of better ways to control malaria through vaccines, drugs, and other prevention strategies. His lab runs a vaccine development project to optimize efficacy of the Duffy Binding Protein (DBP) as a broadly effective vaccine that elicits antibodies to strain-transcending neutralizing epitopes. He is also working on a large scale transposon mutagenesis screen using whole genome piggyBac insertional mutagenesis of P. falciparum to experimentally identify and validate critical processes and pathways that are novel anti-malarial drug targets. Chemogenetic profiling is used to define critical pathways linked to Artemisinin resistance, understand the mechanism of action of antimalarial drugs, and predict optimal drug combination therapies. Additionally, his lab uses functional genomic studies to identify essential genes of P. falciparum through whole genomic piggyBac mutagenesis and genotype-phenotype associations using forward genetic screens.

    Dr. Adams has more than 120 publications in peer-reviewed scientific journals. He serves as Editor for Infection and Immunity and acts as an ad-hoc reviewer for 11 other journals. His lab is supported by funding from the National Institutes of Health and the Bill and Melinda Gates Foundations.

    More Information:
    Dr. Adams' faculty page
    Department of Global Health
  • Courses

    • Independent Study: Public Health
    • Directed Research
  • Current Funding

    Immunological characterization of the Plasmodium vivax Duffy Binding Protein
    NIH/NIAID/R01AI064478​

    A large-scale transposon mutagenesis screen of Plasmodium falciparum
    NIH/NIAID/R01AI094973

    Chemogenomic Profiling of Plasmodium Falciparum Drug Responses and Resistance
    NIH/NIAID/R01AI117017

    B Cell Epitope Mapping of Viral and Parasitic Antigens
    WashingtonUniversity/NIAID/HHSN272201400018C

    Long-term Continuous Culture of Plasmodium Vivax Blood Stages
    Bill and Melinda Gates Foundation/OPP1023643 ​

    3D Microfluidic Human Liver Models for Malaria Drugs
    Bill and Melinda Gates Foundation/OPP1023601
  • Recent Publications

    Bronner IF, Otto TD, Zhang M, Udenze K, Wang C, Quail MA, Jiang RHY, Adams JH, Rayner JC. 2016. Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants. Genome Res 26:980–9.  

    Chen E, Salinas ND, Huang Y, Ntumngia F, Plasencia MD, Gross ML, Adams JH, Tolia NH. 2016. Broadly neutralizing epitopes in the Plasmodium vivax vaccine candidate Duffy Binding Protein. Proc Natl Acad Sci U S A 113:6277–82.  

    Pradhan A, Siwo GH, Singh N, Martens B, Balu B, Button-Simons KA, Tan A, Zhang M, Udenze KO, Jiang RHY, Ferdig MT, Adams JH, Kyle DE. 2015. Chemogenomic profiling of Plasmodium falciparum as a tool to aid antimalarial drug discovery. Sci Rep 5:15930.  

    Mikolajczak SA, Vaughan AM, Kangwanrangsan N, Roobsoong W, Fishbaugher M, Yimamnuaychok N, Rezakhani N, Lakshmanan V, Singh N, Kaushansky A, Camargo N, Baldwin M, Lindner SE, Adams JH, Sattabongkot J, Prachumsri J, Kappe SHI. 2015. Plasmodium vivax liver stage development and hypnozoite persistence in human liver-chimeric mice. Cell Host Microbe 17:526–35.  

    Lupton EJ, Roth A, Patrapuvich R, Maher SP, Singh N, Sattabongkot J, Adams JH. 2015. Enhancing longevity of Plasmodium vivax and P. falciparum sporozoites after dissection from mosquito salivary glands. Parasitol Int 64:211–218.  

    Chootong P, McHenry AM, Ntumngia FB, Sattabongkot J, Adams JH. 2014. The association of Duffy binding protein region II polymorphisms and its antigenicity in Plasmodium vivax isolates from Thailand. Parasitol Int 63:858–64.  

    Souza-Silva FA, Torres LM, Santos-Alves JR, Tang ML, Sanchez BAM, Sousa TN, Fontes CJF, Nogueira PA, Rocha RS, Brito CFA, Adams JH, Kano FS, Carvalho LH. 2014. Duffy antigen receptor for chemokine (DARC) polymorphisms and its involvement in acquisition of inhibitory anti-duffy binding protein II (DBPII) immunity. PLoS One 9:e93782.  

    Campbell CO, Santiago DN, Guida WC, Manetsch R, Adams JH. 2014. In silico characterization of an atypical MAPK phosphatase of Plasmodium falciparum as a suitable target for drug discovery. Chem Biol Drug Des 84:158–68.  

    Ntumngia FB, Schloegel J, McHenry AM, Barnes SJ, George MT, Kennedy S, Adams JH. 2013. Immunogenicity of single versus mixed allele vaccines of Plasmodium vivax Duffy binding protein region II. Vaccine 31:4382–8.  

    Handa S, Ramamoorthy D, Spradling TJ, Guida WC, Adams JH, Bendinskas KG, Merkler DJ. 2013. Production of recombinant 1-deoxy-d-xylulose-5-phosphate synthase from Plasmodium vivax in Escherichia coli. FEBS Open Bio 3:124–9.  

    Auliff AM, Balu B, Chen N, O’Neil MT, Cheng Q, Adams JH. 2012. Functional analysis of Plasmodium vivax dihydrofolate reductase-thymidylate synthase genes through stable transformation of Plasmodium falciparum. PLoS One 7:e40416.  

    Ntumngia FB, Schloegel J, Barnes SJ, McHenry AM, Singh S, King CL, Adams JH. 2012. Conserved and variant epitopes of Plasmodium vivax Duffy binding protein as targets of inhibitory monoclonal antibodies. Infect Immun 80:1203–8.  

    King CL, Adams JH, Xianli J, Grimberg BT, McHenry AM, Greenberg LJ, Siddiqui A, Howes RE, da Silva-Nunes M, Ferreira MU, Zimmerman PA. 2011. Fy(a)/Fy(b) antigen polymorphism in human erythrocyte Duffy antigen affects susceptibility to Plasmodium vivax malaria. Proc Natl Acad Sci U S A 108:20113–8. 

    Balu B, Maher SP, Pance A, Chauhan C, Naumov A V, Andrews RM, Ellis PD, Khan SM, Lin J-W, Janse CJ, Rayner JC, Adams JH. 2011. CCR4-associated factor 1 coordinates the expression of Plasmodium falciparum egress and invasion proteins. Eukaryot Cell 10:1257–63.  

    Fonager J, Franke-Fayard BMD, Adams JH, Ramesar J, Klop O, Khan SM, Janse CJ, Waters AP. 2011. Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites. BMC Genomics 12:155.  



Michael White , Ph.D.

Michael White, PhD

Deputy Director, Center for Drug Discovery and Innovation; Professor, Department of Global Health     
Genomics approach to understanding Toxoplasmosis
Office: IDRB, 325
Email: mwhite3@health.usf.edu
  • Biography

    Dr. White is a Distinguished USF Health Professor in the Department of Global Health, College of Public Health.  In addition, Dr. White serves as Deputy Director for the Center for Drug Discovery and Innovation and is Co-Director of the newly accredited USF Genetic Counseling Program.  He received his PhD in microbiology from Oregon State University. His lab focuses on how the malaria-related protozoa, Toxoplasma gondii. Projects in his laboratory focus on how these parasites interact with their host cell to produce new progeny and establish persistent infections. Employing functional genomic methods, he has defined the cell cycle and developmental transcriptomes of these parasites and is studying a unique set of plant-like transcription factors, called ApiAP2 proteins, that are responsible for controlling parasite development in the human host.  The White lab uses forward genetic approaches to identify key factors responsible for regulating the complex cell cycle of these parasites.  Whole genome sequencing of a large collection of parasite mutants, has led to the discovery of novel factors that are essential for parasite replication.

    Dr. White has over 100 peer-reviewed publications and serves as a reviewer for several journals. He is the immediate past Chair of the NIH Pathogenic Eukaryotic Study Section and a member of the Genome Consortium for Toxoplasma gondii.

    More Information:
    Dr. White's faculty page
    Department of Global Health
    CDDI

     

  • Courses

    • Human Genomics in Medicine and Public Health PHC6601
      • This course will introduce genomics and modern genetic technologies to health students who have limited training in molecular biology and biochemistry. The course will integrate these rapidly developing technologies into the real world of personal health decisions and public health initiatives.
  • Current Funding

    Developmental Switches Regulating Tissue Cyst Formation
    NIH/NIAID/R56AI124682  

    Studies of DNA Licensing in Apicomplexa Parasites
    NIH/NIAID/R01AI122760  

    Centrosome Control of Toxoplasma Growth
    NIH/NIAID/R01AI109843  

    The AP2 Factors Required for Toxoplasma Replication
    NIH/NIAID/R01AI089885
  • Recent Publications

    Lorenzi H, Khan A, Behnke MS, Namasivayam S, Swapna LS, Hadjithomas M, Karamycheva S, Pinney D, Brunk BP, Ajioka JW, Ajzenberg D, Boothroyd JC, Boyle JP, Dardé ML, Diaz-Miranda MA, Dubey JP, Fritz HM, Gennari SM, Gregory BD, Kim K, Saeij JPJ, Su C, White MW, Zhu X-Q, Howe DK, Rosenthal BM, Grigg ME, Parkinson J, Liu L, Kissinger JC, Roos DS, Sibley LD. 2016. Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nat Commun 7:10147.

    El Bissati K, Suvorova ES, Xiao H, Lucas O, Upadhya R, Ma Y, Angeletti RH, White MW, Weiss LM, Kim K. 2016. Toxoplasma gondii Arginine Methyltransferase 1 (PRMT1) Is Necessary for Centrosome Dynamics during Tachyzoite Cell Division. MBio 7:e02094-15.  

    Engelberg K, Ivey FD, Lin A, Kono M, Lorestani A, Faugno-Fusci D, Gilberger T-W, White M, Gubbels M-J. 2016. A MORN1-associated HAD phosphatase in the basal complex is essential for Toxoplasma gondii daughter budding. Cell Microbiol 18:1153–71.  

    Sugi T, Ma YF, Tomita T, Murakoshi F, Eaton MS, Yakubu R, Han B, Tu V, Kato K, Kawazu S-I, Gupta N, Suvorova ES, White MW, Kim K, Weiss LM. 2016. Toxoplasma gondii Cyclic AMP-Dependent Protein Kinase Subunit 3 Is Involved in the Switch from Tachyzoite to Bradyzoite Development. MBio 7.  

    Suvorova ES, Francia M, Striepen B, White MW. 2015. A novel bipartite centrosome coordinates the apicomplexan cell cycle. PLoS Biol 13:e1002093.  

    Vinayak S, Brooks CF, Naumov A, Suvorova ES, White MW, Striepen B. 2014. Genetic manipulation of the Toxoplasma gondii genome by fosmid recombineering. MBio 5:e02021.  

    Brown KM, Suvorova E, Farrell A, McLain A, Dittmar A, Wiley GB, Marth G, Gaffney PM, Gubbels MJ, White M, Blader IJ. 2014. Forward genetic screening identifies a small molecule that blocks Toxoplasma gondii growth by inhibiting both host- and parasite-encoded kinases. PLoS Pathog 10:e1004180.  

    Croken MM, Qiu W, White MW, Kim K. 2014. Gene Set Enrichment Analysis (GSEA) of Toxoplasma gondii expression datasets links cell cycle progression and the bradyzoite developmental program. BMC Genomics 15:515.  

    Suvorova ES, Croken M, Kratzer S, Ting L-M, Conde de Felipe M, Balu B, Markillie ML, Weiss LM, Kim K, White MW. 2013. Discovery of a splicing regulator required for cell cycle progression. PLoS Genet 9:e1003305.  

    Radke JB, Lucas O, De Silva EK, Ma Y, Sullivan WJ, Weiss LM, Llinas M, White MW. 2013. ApiAP2 transcription factor restricts development of the Toxoplasma tissue cyst. Proc Natl Acad Sci U S A 110:6871–6.  

    Suvorova ES, Radke JB, Ting L-M, Vinayak S, Alvarez CA, Kratzer S, Kim K, Striepen B, White MW. 2013. A nucleolar AAA-NTPase is required for parasite division. Mol Microbiol 90:338–55.  

    Szatanek T, Anderson-White BR, Faugno-Fusci DM, White M, Saeij JPJ, Gubbels M-J. 2012. Cactin is essential for G1 progression in Toxoplasma gondii. Mol Microbiol 84:566–77.  

    Suvorova ES, Lehmann MM, Kratzer S, White MW. 2012. Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii. Mol Biochem Parasitol 181:7–16.  

    A complete list of publications can be found at the following URL address: http://www.ncbi.nlm.nih.gov/sites/myncbi/michael.white.2/bibliography/41152274/public/?sort=date&direction=ascending  



Lindsey Shaw

Lindsey 'Les' Shaw, PhD

Director of Graduate Studies, Department of Cell Biology, Microbiology, and Molecular Biology     
Bacterial pathogenesis and antibacterial drug discovery
Office: ISA 6204
Email: shaw@usf.edu
  • Biography

    Dr. Shaw is the Director of Graduate Studies in the Department of Cell Biology, Microbiology, and Molecular Biology. He received his PhD in molecular microbiology from the University of Sheffield in England. He joined USF in 2007 as an assistant professor. He became director of graduate studies in spring 2009, was promoted to Associate Professor with tenure during the summer of 2012, and became a Full Professor in summer 2016. The Shaw lab works to understand the mechanisms bacteria use in the infection process with a goal of developing new treatments options that minimize the emergence of resistance. They place a specific emphasis on Methicillin Resistant Staphylococcus aureus (MRSA), although they also study other members of the ESKAPE pathogens as well. More specifically, they use molecular tools to investigate regulatory elements and how they coordinate and module the progression of infection and the roll of proteolytic enzymes in the virulence process. His lab also runs a drug discovery program to discovery new antibacterial drugs to combat ESKAPE pathogens.

    More Information:
    Dr.Shaw's faculty page
    Department of Cell Biology, Microbiology, and Molecular Biology 

  • Courses

    • Bacteria Pathogenesis & Resistance BSC 6932
    • Advances in Cell and Molecular Biology PCB 6920
      • A journal club in which graduate students present and discuss research publications from the preceding twelve months in the fields of molecular and cellular biology.
    • Molecular Microbiology MCB 4320
      • Subjects covered include: bacterial pathogenesis, mechanisms of genetic control, horizontal gene transfer, bacteriophage biology, DHA mutagenesis and repair, antibiotic resistance
    • Microbiology undergraduate research
    • Directed Research
    • Thesis: Master’s Dissertation: Doctoral
  • Current Funding

    Exploring the Influence of an Intracellular Aminopeptidase on Staphylococcus Aureus Virulence
    NIH/NIAID/R21AI109389
  • Recent Publications

    Carroll RK, Weiss A, Broach WH, Wiemels RE, Mogen AB, Rice KC, Shaw LN. 2016. Genome-wide Annotation, Identification, and Global Transcriptomic Analysis of Regulatory or Small RNA Gene Expression in Staphylococcus aureus. MBio 7:e01990-15.

    Broach WH, Shaw LN, Weiss A, Lee MC. 2016. Towards the complete small RNome of Acinetobacter baumannii. Microb Genomics 2.  

    Carroll RK, Weiss A, Shaw LN. 2016. RNA-Sequencing of Staphylococcus aureus Messenger RNA. Methods Mol Biol 1373:131–41.  

    Bionda N, Fleeman RM, de la Fuente-Núñez C, Rodriguez MC, Reffuveille F, Shaw LN, Pastar I, Davis SC, Hancock REW, Cudic P. 2016. Identification of novel cyclic lipopeptides from a positional scanning combinatorial library with enhanced antibacterial and antibiofilm activities. Eur J Med Chem 108:354–63.  

    Weiss A, Shaw LN. 2015. Small things considered: the small accessory subunits of RNA polymerase in Gram-positive bacteria. FEMS Microbiol Rev 39:541–54.

    Krute CN, Carroll RK, Rivera FE, Weiss A, Young RM, Shilling A, Botlani M, Varma S, Baker BJ, Shaw LN. 2015. The disruption of prenylation leads to pleiotropic rearrangements in cellular behavior in Staphylococcus aureus. Mol Microbiol 95:819–32.  

    Fleeman R, LaVoi TM, Santos RG, Morales A, Nefzi A, Welmaker GS, Medina-Franco JL, Giulianotti MA, Houghten RA, Shaw LN. 2015. Combinatorial Libraries As a Tool for the Discovery of Novel, Broad-Spectrum Antibacterial Agents Targeting the ESKAPE Pathogens. J Med Chem 58:3340–3355.  

    Burda WN, Miller HK, Krute CN, Leighton SL, Carroll RK, Shaw LN. 2014. Investigating the genetic regulation of the ECF sigma factor σS in Staphylococcus aureus. BMC Microbiol 14:280.  

    Van Horn KS, Burda WN, Fleeman R, Shaw LN, Manetsch R. 2014. Antibacterial Activity of a Series of N 2 , N 4 -Disubstituted Quinazoline-2,4-diamines. J Med Chem 57:3075–3093.  

    Weiss A, Ibarra JA, Paoletti J, Carroll RK, Shaw LN. 2014. The δ subunit of RNA polymerase guides promoter selectivity and virulence in Staphylococcus aureus. Infect Immun 82:1424–35  

    Carroll RK, Veillard F, Gagne DT, Lindenmuth JM, Poreba M, Drag M, Potempa J, Shaw LN. 2013. The Staphylococcus aureus leucine aminopeptidase is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine. Biol Chem 394:791–803.  

    Rivera FE, Miller HK, Kolar SL, Stevens SM, Shaw LN. 2012. The impact of CodY on virulence determinant production in community-associated methicillin-resistant Staphylococcus aureus. Proteomics 12:263–8.  

    Beau J, Mahid N, Burda WN, Harrington L, Shaw LN, Mutka T, Kyle DE, Barisic B, van Olphen A, Baker BJ. 2012. Epigenetic tailoring for the production of anti-infective cytosporones from the marine fungus Leucostoma persoonii. Mar Drugs 10:762–74.    



Rays Jiang, Ph.D

Rays Jiang, PhD

Assistant Professor, Department of Global Health and Center for Drug discovery and Innovation     
Computational and integrative biology approach to disease
Office: IDRB 402
Email: Jiang2@health.usf.edu
  • Biography

    Dr. Rays Jiang specializes in systems biology in the Global Health (GH) department and Center for Drug Discovery and Innovation (CDDI) in USF. She received her PhD in genomics from Wageningen University in the Netherlands. The Jiang lab uses computational biology to analyze and integrate diverse large-scale genomics data sets of infectious diseases. She uses genome, transcriptome, epigenome, and population genomics data to understand mechanisms that are critical for pathogen growth. The lab is using gene network analysis and evolutionary trajectory studies to identify the most unique and amenable pathways in pathogens for drug intervention, while simultaneously creating a roadmap of metabolic and signaling changes in the human cell. She is also working to create Cloud-based computational pipelines that can be used to combat existing and emerging infectious diseases with new diagnostic, tracking, and analysis tools. Dr. Jiang has an impressive publication history and is a recipient of multiple research funding awards.  

    More Information:
    Dr. Jiang's faculty page
    Rays Jiang Lab
    Department of Global health
    CDDI  

  • Courses

    • PHC 6934 – Quantitative Genomics and Genetics
      • This course will introduce quantitative genetic and genomic concepts and skills to health students. Through real world case studies and student projects, students will develop effective analytical skills to handle fundamental problems in genomics.
    • Supervised Field Experience
  • Current Funding

    Chemogenomic Profiling of Plasmodium Falciparum Drug Responses and Resistance
    NIH/NIAID/R01AI117017  

    Targeting Mutant KRas for Cancer Therapy
    NIH/NCI/R35CA197731  

    3D Microfluidic Human Liver Models for Malaria Drugs
    Bill and Melinda Gates Foundation/OPP1023601 ​

    Exploring Third Generation Sequencing Technology for Malaria Genomics
    New Investigator Research Award of USF
  • Recent Publications

    Bronner IF, Otto TD, Zhang M, Udenze K, Wang C, Quail MA, Jiang RHY, Adams JH, Rayner JC. 2016. Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants. Genome Res 26:980–9.

    Dankwa S, Lim C, Bei AK, Jiang RHY, Abshire JR, Patel SD, Goldberg JM, Moreno Y, Kono M, Niles JC, Duraisingh MT. 2016. Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite. Nat Commun 7:11187  

    Egan ES, Jiang RHY, Moechtar MA, Barteneva NS, Weekes MP, Nobre L V, Gygi SP, Paulo JA, Frantzreb C, Tani Y, Takahashi J, Watanabe S, Goldberg J, Paul AS, Brugnara C, Root DE, Wiegand RC, Doench JG, Duraisingh MT. 2015. Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion. Science 348:711–4.  

    Merrick CJ, Jiang RHY, Skillman KM, Samarakoon U, Moore RM, Dzikowski R, Ferdig MT, Duraisingh MT. 2015. Functional analysis of sirtuin genes in multiple Plasmodium falciparum strains. PLoS One 10:e0118865.  

    Paul AS, Saha S, Engelberg K, Jiang RHY, Coleman BI, Kosber AL, Chen C-T, Ganter M, Espy N, Gilberger TW, Gubbels M-J, Duraisingh MT. 2015. Parasite Calcineurin Regulates Host Cell Recognition and Attachment by Apicomplexans. Cell Host Microbe 18:49–60.  

    Coleman BI, Skillman KM, Jiang RHY, Childs LM, Altenhofen LM, Ganter M, Leung Y, Goldowitz I, Kafsack BFC, Marti M, Llinás M, Buckee CO, Duraisingh MT. 2014. A Plasmodium falciparum histone deacetylase regulates antigenic variation and gametocyte conversion. Cell Host Microbe 16:177–86.  

    Jiang RHY, de Bruijn I, Haas BJ, Belmonte R, Löbach L, Christie J, van den Ackerveken G, Bottin A, Bulone V, Díaz-Moreno SM, Dumas B, Fan L, Gaulin E, Govers F, Grenville-Briggs LJ, Horner NR, Levin JZ, Mammella M, Meijer HJG, Morris P, Nusbaum C, Oome S, Phillips AJ, van Rooyen D, Rzeszutek E, Saraiva M, Secombes CJ, Seidl MF, Snel B, Stassen JHM, Sykes S, Tripathy S, van den Berg H, Vega-Arreguin JC, Wawra S, Young SK, Zeng Q, Dieguez-Uribeondo J, Russ C, Tyler BM, van West P. 2013. Distinctive expansion of potential virulence genes in the genome of the oomycete fish pathogen Saprolegnia parasitica. PLoS Genet 9:e1003272.  

    Jiang RHY, Stahelin R V, Bhattacharjee S, Haldar K. 2013. Eukaryotic virulence determinants utilize phosphoinositides at the ER and host cell surface. Trends Microbiol 21:145–56.  

    Neafsey DE, Galinsky K, Jiang RHY, Young L, Sykes SM, Saif S, Gujja S, Goldberg JM, Young S, Zeng Q, Chapman SB, Dash AP, Anvikar AR, Sutton PL, Birren BW, Escalante AA, Barnwell JW, Carlton JM. 2012. The malaria parasite Plasmodium vivax exhibits greater genetic diversity than Plasmodium falciparum. Nat Genet 44:1046–50.  

    Jiang RHY, Tyler BM. 2012. Mechanisms and evolution of virulence in oomycetes. Annu Rev Phytopathol 50:295–318.  

    Jiang RHY, Marti M. 2012. A PIP Gets the plasmodium protein export pathway going. Cell Host Microbe 11:99–100.  

    Raffaele S, Farrer RA, Cano LM, Studholme DJ, MacLean D, Thines M, Jiang RHY, Zody MC, Kunjeti SG, Donofrio NM, Meyers BC, Nusbaum C, Kamoun S. 2010. Genome Evolution Following Host Jumps in the Irish Potato Famine Pathogen Lineage. Science (80- ) 330.  

    Baxter L, Tripathy S, Ishaque N, Boot N, Cabral A, Kemen E, Thines M, Ah-Fong A, Anderson R, Badejoko W, Bittner-Eddy P, Boore JL, Chibucos MC, Coates M, Dehal P, Delehaunty K, Dong S, Downton P, Dumas B, Fabro G, Fronick C, Fuerstenberg SI, Fulton L, Gaulin E, Govers F, Hughes L, Humphray S, Jiang RHY, Judelson H, Kamoun S, Kyung K, Meijer H, Minx P, Morris P, Nelson J, Phuntumart V, Qutob D, Rehmany A, Rougon-Cardoso A, Ryden P, Torto-Alalibo T, Studholme D, Wang Y, Win J, Wood J, Clifton SW, Rogers J, Van den Ackerveken G, Jones JDG, McDowell JM, Beynon J, Tyler BM. 2010. Signatures of Adaptation to Obligate Biotrophy in the Hyaloperonospora arabidopsidis Genome. Science (80- ) 330.  

    Jiang RHY, Tripathy S, Govers F, Tyler BM. 2008. RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members. Proc Natl Acad Sci U S A 105:4874–9.  

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