The purpose of this controlled experimental design cell culture model study is to measure the transforming activities of Human Papillomavirus (HPV) 16 E7 & E6. In vitro transformation will be conducted while transfecting HPV 16 E7 & E6 oncogenes into primary, baby mouse kidney (BMK) cells. Primary, BMK cells will then be co-transfected with HPV 14 & 21 E6 proteins to determine the effect on the oncogenic activities of HPV 16 oncogenes.
Public Health Relevance
These laboratory experiments should lead to important insights into strategies that may be used to inhibit the oncogenic activity of HR HPVs.