Kay-Pong DanielYip

Kay-Pong Daniel Yip, Ph.D.

Professor, College Of Medicine Molecular Pharmacology & Physiology

College Of Medicine Molecular Pharmacology & Physiology

Director, Molecular Pharmacology and Physiology Seminar Series, University of South Florida

Contact Info 12901 Bruce B. Downs Blvd.
MDC 8
Tampa, FL 33612

Academic Email: dyip@health.usf.edu

Academic Phone: (813) 974-7111

Education

  • PHD, Physiology & Biophysics, University Of Southern California, 1991
  • B.S. (Honors), Biology, Chinese University of Hong Kong, 1984

Interdisciplinary and Emerging Signature Programs

  • Cancer Biology
  • Cardiovascular
  • Cellular and Molecular Biology
  • Pulmonary Physiology

Research Interests

  • The research interests of our laboratory are to develop and apply confocal fluorescence microscopy, laser-based video microscopy, and flash photolysis of caged compounds to study renal cellular function in situ and in vivo. Currently we are investigating the regulation of water channels (aquaporin-2) trafficking in kidney collecting duct by antidiuretic hormone (vasopressin), the role of integrins and Ca2+ microdomains in mechanical signal transduction in renal vascular smooth muscle, and cellular mechanisms of pressure natriuresis and its relationship with the development of hypertension.

  • In addition, we're studying the regulation of blood pressure and renal functions, with emphasis in developing novel imaging techniques for in vivo and in situ studies, and using interdisciplinary approaches to study the physiological phenomena from subcellular to organ level. There are two active projects in lab, which are supported by NIH and AHA.

  • 1. Intracellular Ca2+ oscillations and trafficking of aquaporin-2 (AQP2) in intact inner medullary collecting duct cells. Confocal fluorescence microscopy allows the study of physiological process in a single cell in a perfused tubule without the complication of out-of-focus-fluorescence signal derived from the other cells. By combining confocal fluorescence microscopy and tubular microperfusion technique, we are the first to demonstrate that vasopressin (AVP) in physiological range ( 0.01  0.1 nM) mobilizes intracellular Ca2+ in form of oscillations in intact inner medullary collecting duct (J. Biol. Chem. 2000). By developing a novel confocal fluorescence imaging technique using a styryl dye (FM1-43) to monitor real time exocytosis of aquaporin-2 in inner medullary collecting duct (IMCD), we provide the first direct evidence that AVP induced AQP2 exocytotic insertion is Ca2+ dependent (J. Physiol. 2002). We also have evidence that AVP uses not only PKA but also EPAC as cAMP receptor to induce AQP2 apical exocytosis (Am. J. Physiol. 2005).

  • 2. Integrin-mediated mechanotransduction in renal vascular smooth muscle cells. Myogenic response and tubuloglomerular feedback are the two major mechanisms responsible to provide renal blood flow autoregulation. But mechanism of mechanotransduction in myogenic response is not known. We hypothesize that increase of transmural pressure (stretch) alters the attachment between extracellular matrix (ECM) and integrins in vascular smooth muscle, and that mechanical force is transduced via integrin to trigger Ca2+ sparks (local subcellular Ca2+ release from ryanodine receptors, detected by high speed confocal microscopy) to initiate and maintain myogenic contraction. We are testing these hypotheses by applying controlled mechanical force using fibronectin-coated paramagnetic beads (or nanoparticles) and monitoring the occurrence of Ca2+ sparks in freshly isolated renal vascular smooth muscles, and examining the effects of transmural pressure in the frequency of Ca2+ sparks in intact renal vascular smooth muscle cells. (Am. J. Physiol 2007)

Memberships

  • American Society of Nephrology (Member, 2008 - Present)
  • American Physiological Society (Member, 2008 - Present)

Recent Publications

  • Balasubramanian, L.Lo, CM.Sham, JS.Yip, KP. Remanent cell traction force in renal vascular smooth muscle cells induced by integrin-mediated mechanotransduction. American journal of physiology. Cell physiology. 304(4) : C382-91, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23325413
  • Yip, KP.Sham, JS. Tracking stars: automated two-dimensional analysis of Ca²⁺ events. Focus on "Automated region of interest analysis of dynamic Ca²⁺ signals in image sequences". American journal of physiology. Cell physiology. 303(3) : C233-5, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22648951
  • Yip, KP.Sham, JS. Mechanisms of vasopressin-induced intracellular Ca2+ oscillations in rat inner medullary collecting duct. American journal of physiology. Renal physiology. 300(2) : F540-8, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21147839
  • Mok, SC.Bonome, T.Vathipadiekal, V.Bell, A.Johnson, ME.Wong, KK.Park, DC.Hao, K.Yip, DK.Donninger, H.Ozbun, L.Samimi, G.Brady, J.Randonovich, M.Pise-Masison, CA.Barrett, JC.Wong, WH.Welch, WR.Berkowitz, RS.Birrer, MJ. A gene signature predictive for outcome in advanced ovarian cancer identifies a survival factor: microfibril-associated glycoprotein 2. Cancer cell. 16(6) : 521-32, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19962670
  • Balasubramanian, L.Sham, JS.Yip, KP. Calcium signaling in vasopressin-induced aquaporin-2 trafficking. Pflügers Archiv : European journal of physiology. 456(4) : 747-54, 2008. http://www.ncbi.nlm.nih.gov/pubmed/17957381
  • Balasubramanian, L.Yip, KP.Hsu, TH.Lo, CM. Impedance Analysis of Renal Vascular Smooth Muscle Cells. American journal of physiology. Cell physiology. , 2008. http://www.ncbi.nlm.nih.gov/pubmed/18684988
  • Ditlevsen, S.Yip, KP.Marsh, DJ.Holstein-Rathlou, NH. Parameter estimation of feedback gain in a stochastic model of renal hemodynamics: differences between spontaneously hypertensive and Sprague-Dawley rats. American journal of physiology. Renal physiology. 292(2) : F607-16, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17018842
  • Sosnovtseva, OV.Pavlov, AN.Mosekilde, E.Yip, KP.Holstein-Rathlou, NH.Marsh, DJ. Synchronization among mechanisms of renal autoregulation is reduced in hypertensive rats. , 2007. http://www.ncbi.nlm.nih.gov/pubmed/17728377
  • Balasubramanian, L.Ahmed, A.Lo, CM.Sham, JS.Yip, KP. Integrin-mediated mechanotransduction in renal vascular smooth muscle cells: activation of calcium sparks. American journal of physiology. Regulatory, integrative and comparative physiology. 293(4) : R1586-94, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17699564
  • Leong, PK.Yang, LE.Landon, CS.McDonough, AA.Yip, KP. Phenol injury-induced hypertension stimulates proximal tubule Na+/H+ exchanger activity. American journal of physiology. Renal physiology. 290(6) : F1543-50, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16390865
  • Umesh, A.Thompson, MA.Chini, EN.Yip, KP.Sham, JS. Integrin ligands mobilize Ca2+ from ryanodine receptor-gated stores and lysosome-related acidic organelles in pulmonary arterial smooth muscle cells. The Journal of biological chemistry. 281(45) : 34312-23, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16963791
  • Yip, KP. Epac-mediated Ca(2+) mobilization and exocytosis in inner medullary collecting duct. American journal of physiology. Renal physiology. 291(4) : F882-90, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16684923
  • Raghavan, R.Chen, X.Yip, KP.Marsh, DJ.Chon, KH. Interactions between TGF-dependent and myogenic oscillations in tubular pressure and whole kidney blood flow in both SDR and SHR. American journal of physiology. Renal physiology. 290(3) : F720-32, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16219915
  • Marsh, DJ.Sosnovtseva, OV.Pavlov, AN.Yip, KP.Holstein-Rathlou, NH. Frequency encoding in renal blood flow regulation. American journal of physiology. Regulatory, integrative and comparative physiology. 288(5) : R1160-7, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15661968
  • Chon, KH.Raghavan, R.Chen, YM.Marsh, DJ.Yip, KP. Interactions of TGF-dependent and myogenic oscillations in tubular pressure. American journal of physiology. Renal physiology. 288(2) : F298-307, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15479856
  • Yip, KP. Flash photolysis of caged nitric oxide inhibits proximal tubular fluid reabsorption in free-flow nephron. American journal of physiology. Regulatory, integrative and comparative physiology. 289(2) : R620-R626, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15790750
  • Yang, XR.Lin, MJ.Yip, KP.Jeyakumar, LH.Fleischer, S.Leung, GP.Sham, JS. Multiple ryanodine receptor subtypes and heterogeneous ryanodine receptor-gated Ca2+ stores in pulmonary arterial smooth muscle cells. American journal of physiology. Lung cellular and molecular physiology. 289(2) : L338-48, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15863441
  • Ditlevsen, S.Yip, KP.Holstein-Rathlou, NH. Parameter estimation in a stochastic model of the tubuloglomerular feedback mechanism in a rat nephron. Mathematical biosciences. 194(1) : 49-69, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15836864
  • Lin, MJ.Leung, GP.Zhang, WM.Yang, XR.Yip, KP.Tse, CM.Sham, JS. Chronic hypoxia-induced upregulation of store-operated and receptor-operated Ca2+ channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension. Circulation research. 95(5) : 496-505, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15256480
  • Zhang, WM.Yip, KP.Lin, MJ.Shimoda, LA.Li, WH.Sham, JS. ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors. American journal of physiology. Lung cellular and molecular physiology. 285(3) : L680-90, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12740215
  • Zou, R.Cupples, WA.Yip, KP.Holstein-Rathlou, NH.Chon, KH. Time-varying properties of renal autoregulatory mechanisms. IEEE transactions on bio-medical engineering. 49(10) : 1112-20, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12374335
  • Yip, KP.Kurtz, I. Confocal fluorescence microscopy measurements of pH and calcium in living cells. Methods in cell biology. 70: 417-27, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12512331
  • Yip, KP. Coupling of vasopressin-induced intracellular Ca2+ mobilization and apical exocytosis in perfused rat kidney collecting duct. The Journal of physiology. 538(Pt 3) : 891-9, 2002. http://www.ncbi.nlm.nih.gov/pubmed/11826172
  • Yip, KP.Tsuruoka, S.Schwartz, GJ.Kurtz, I. Apical H(+)/base transporters mediating bicarbonate absorption and pH(i) regulation in the OMCD. American journal of physiology. Renal physiology. 283(5) : F1098-104, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12372786
  • Chan, WL.Holstein-Rathlou, NH.Yip, KP. Integrin mobilizes intracellular Ca(2+) in renal vascular smooth muscle cells. American journal of physiology. Cell physiology. 280(3) : C593-603, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11171579
  • Yip, KP.Wagner, AJ.Marsh, DJ. Detection of apical Na(+)/H(+) exchanger activity inhibition in proximal tubules induced by acute hypertension. American journal of physiology. Regulatory, integrative and comparative physiology. 279(4) : R1412-8, 2000. http://www.ncbi.nlm.nih.gov/pubmed/11004011
  • Chou, CL.Yip, KP.Michea, L.Kador, K.Ferraris, JD.Wade, JB.Knepper, MA. Regulation of aquaporin-2 trafficking by vasopressin in the renal collecting duct. Roles of ryanodine-sensitive Ca2+ stores and calmodulin. The Journal of biological chemistry. 275(47) : 36839-46, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10973964
  • Pushkin, A.Yip, KP.Clark, I.Abuladze, N.Kwon, TH.Tsuruoka, S.Schwartz, GJ.Nielsen, S.Kurtz, I. NBC3 expression in rabbit collecting duct: colocalization with vacuolar H+-ATPase. The American journal of physiology. 277(6 Pt 2) : F974-81, 1999. http://www.ncbi.nlm.nih.gov/pubmed/10600945
  • Yip, KP.Tse, CM.McDonough, AA.Marsh, DJ. Redistribution of Na+/H+ exchanger isoform NHE3 in proximal tubules induced by acute and chronic hypertension. The American journal of physiology. 275(4 Pt 2) : F565-75, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9755128
  • Cutaia, MV.Parks, N.Centracchio, J.Rounds, S.Yip, KP.Sun, AM. Effect of hypoxic exposure on Na+/H+ antiport activity, isoform expression, and localization in endothelial cells. The American journal of physiology. 275(3 Pt 1) : L442-51, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9728038
  • Quayle, AJ.Porter, EM.Nussbaum, AA.Wang, YM.Brabec, C.Yip, KP.Mok, SC. Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. The American journal of pathology. 152(5) : 1247-58, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9588893
  • Yip, KP.Marsh, DJ. An Arg-Gly-Asp peptide stimulates constriction in rat afferent arteriole. The American journal of physiology. 273(5 Pt 2) : F768-76, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9374840
  • Sun, AM.Liu, Y.Dworkin, LD.Tse, CM.Donowitz, M.Yip, KP. Na+/H+ exchanger isoform 2 (NHE2) is expressed in the apical membrane of the medullary thick ascending limb. The Journal of membrane biology. 160(1) : 85-90, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9351894
  • Yip, KP.Holstein-Rathlou, NH. Chaos and non-linear phenomena in renal vascular control. Cardiovascular research. 31(3) : 359-70, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8681323
  • Yip, KP.Marsh, DJ. [Ca2+]i in rat afferent arteriole during constriction measured with confocal fluorescence microscopy. The American journal of physiology. 271(5 Pt 2) : F1004-11, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8945994
  • Zhang, Y.Mircheff, AK.Hensley, CB.Magyar, CE.Warnock, DG.Chambrey, R.Yip, KP.Marsh, DJ.Holstein-Rathlou, NH.McDonough, AA. Rapid redistribution and inhibition of renal sodium transporters during acute pressure natriuresis. The American journal of physiology. 270(6 Pt 2) : F1004-14, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8764320
  • Yip, KP.Kurtz, I. NH3 permeability of principal cells and intercalated cells measured by confocal fluorescence imaging. The American journal of physiology. 269(4 Pt 2) : F545-50, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7485540
  • Chen, YM.Yip, KP.Marsh, DJ.Holstein-Rathlou, NH. Magnitude of TGF-initiated nephron-nephron interactions is increased in SHR. The American journal of physiology. 269(2 Pt 2) : F198-204, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7653593
  • Yip, KP.Holstein-Rathlou, NH.Marsh, DJ. Mechanisms of temporal variation in single-nephron blood flow in rats. The American journal of physiology. 264(3 Pt 2) : F427-34, 1993. http://www.ncbi.nlm.nih.gov/pubmed/8456956
  • Smedley, G.Yip, KP.Wagner, A.Dubovitsky, S.Marsh, DJ. A laser Doppler instrument for in vivo measurements of blood flow in single renal arterioles. IEEE transactions on bio-medical engineering. 40(3) : 290-7, 1993. http://www.ncbi.nlm.nih.gov/pubmed/8335333
  • Yip, KP.Holstein-Rathlou, NH.Marsh, DJ. Dynamics of TGF-initiated nephron-nephron interactions in normotensive rats and SHR. The American journal of physiology. 262(6 Pt 2) : F980-8, 1992. http://www.ncbi.nlm.nih.gov/pubmed/1621821
  • Yip, KP.Holstein-Rathlou, NH.Marsh, DJ. Chaos in blood flow control in genetic and renovascular hypertensive rats. The American journal of physiology. 261(3 Pt 2) : F400-8, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1887904
  • Buchanan, TA.Sipos, GF.Gadalah, S.Yip, KP.Marsh, DJ.Hsueh, W.Bergman, RN. Glucose tolerance and insulin action in rats with renovascular hypertension. Hypertension. 18(3) : 341-7, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1889847
  • Marsh, DJ.Yip, KP.Källskog, O.Holstein-Rathlou, NH. Oscillations and more complex dynamics in tubuloglomerular feedback. Kidney international. Supplement. 32: S94-7, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1881062

Awards/Honors

  • National Kidney Foundation Young Investigator Grant (National Kidney Foundation - 1994)
  • Clifford and Evelyn Cherry Fellowship Award (American Heart Association - 1992)
  • American Heart Association Postdoctoral Fellowship (American Heart Association - 1992)