EricBennett

Eric Bennett, Ph.D.

Professor, College Of Medicine Molecular Pharmacology & Physiology

College Of Medicine Molecular Pharmacology & Physiology

Professor, Molecular Pharmacology & Physiology, University of South Florida

Director, Ph.D. Program in Integrated Biomedical Sciences, College of Medicine, University of South Florida

Vice Chair, Molecular Pharmacology & Physiology, University of South Florida

Contact Info 12901 Bruce B. Downs Blvd.
MDC 8
Tampa, FL 33612

Academic Email: esbennet@health.usf.edu

Academic Phone: (813) 974-1545

Education

  • PHD, Biophysics, University Of Rochester, 1992
  • M.S., Biophysics, University of Rochester School of Medicine and Dentistry, 1989
  • B.S., Applied & Engr. Physics, Cornell University, 1985

Interdisciplinary and Emerging Signature Programs

  • Biomedical Engineering & Nanomedicine
  • Cardiovascular
  • Cardiovascular Sciences
  • Metabolic Regulation and Disorders
  • Neuroscience

Research Interests

  • We are interested in determining how neuronal, cardiac and skeletal muscle excitability is regulated through modulation of voltage-gated ion channel function. We have developed two programs within this area: 1) Studies designed to question how glycosylation and its regulation modulate ion channel function, and 2) Studies designed to determine how structural differences among channel isoforms confer isoform-specific functions. In addition, we are exploring other areas, including prostate cancer metastasis. Techniques routinely used by my laboratory include primary and immortal cell culture methods, protein expression, protein biochemistry and immunology using gel electrophoresis and immunocytochemistry technologies, including basic confocal microscopy, molecular biological methods, and electrophysiological recordings. Studies on transgenic and knockout mice used to determine the in vivo role of channel glycosylation on ion channel function, on cardiac and neuronal action potentials, and on conduction using ECG, EEG, optical mapping, and in vivo electrophysiology measurements are also done routinely in the lab. We have added transcriptomic and proteomic methods to determine N-glycan structures attached to neuronal and myocyte membranes to our technical repertoire.

Memberships

  • Society for Glycobiology (Member, 2007 - Present)
  • Council on Basic Cardiovascular Sciences, American Heart Association (Member, 2001 - Present)
  • Society for Neuroscience (Member, 1998 - Present)
  • Biophysical Society (Member, 1990 - Present)
  • American Physiological Society (Member, 1990 - Present)

Recent Publications

  • Du, D.Yang, H.Norring, SA.Bennett, ES. In-silico modeling of glycosylation modulation dynamics in HERG ion channels and cardiac electrical signals. IEEE journal of biomedical and health informatics. 18(1) : 205-14, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24403418
  • Everitt, H.Hu, M.Ajmo, JM.Rogers, CQ.Liang, X.Zhang, R.Yin, H.Choi, A.Bennett, ES.You, M. Ethanol administration exacerbates the abnormalities in hepatic lipid oxidation in genetically obese mice. American journal of physiology. Gastrointestinal and liver physiology. 304(1) : G38-47, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23139221
  • Norring, SA.Ednie, AR.Schwetz, TA.Du, D.Yang, H.Bennett, ES. Channel sialic acids limit hERG channel activity during the ventricular action potential. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(2) : 622-31, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23139156
  • Ednie, AR.Horton, KK.Wu, J.Bennett, ES. Expression of the sialyltransferase, ST3Gal4, impacts cardiac voltage-gated sodium channel activity, refractory period and ventricular conduction. Journal of molecular and cellular cardiology. 59: 117-27, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23471032
  • Panguluri, SK.Tur, J.Fukumoto, J.Deng, W.Sneed, KB.Kolliputi, N.Bennett, ES.Tipparaju, SM. Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle. American journal of physiology. Heart and circulatory physiology. 304(12) : H1651-61, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23585127
  • Ednie, AR.Bennett, ES. Modulation of voltage-gated ion channels by sialylation. Comprehensive Physiology. 2(2) : 1269-301, 2012. http://www.ncbi.nlm.nih.gov/pubmed/23798301
  • Bahia PK, Bennett ES, Taylor-Clark TE Reductions in external divalent cations evoke novel voltage-gated currents in sensory neurons PLoS One. 7(2) : e31585, 2012.
  • Schwetz, TA.Norring, SA.Ednie, AR.Bennett, ES. Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating. The Journal of biological chemistry. 286(6) : 4123-32, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21115483
  • D. Du, H. Yang, S. Norring, E. S. Bennett Multi-Scale Modeling of Glycosylation Modulation Dynamics in Cardiac Electrical Signaling Conf Proc IEEE Eng Med Biol Soc. : 104-7, 2011.
  • Schwetz, TA.Norring, SA.Bennett, ES. N-glycans modulate K(v)1.5 gating but have no effect on K(v)1.4 gating. Biochimica et biophysica acta. , 2009. http://www.ncbi.nlm.nih.gov/pubmed/19961828
  • Montpetit, ML.Stocker, PJ.Schwetz, TA.Harper, JM.Norring, SA.Schaffer, L.North, SJ.Jang-Lee, J.Gilmartin, T.Head, SR.Haslam, SM.Dell, A.Marth, JD.Bennett, ES. Regulated and aberrant glycosylation modulate cardiac electrical signaling. Proceedings of the National Academy of Sciences of the United States of America. 106(38) : 16517-22, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19666501
  • Johnson, D.Bennett, ES. Gating of the shaker potassium channel is modulated differentially by N-glycosylation and sialic acids. Pflügers Archiv : European journal of physiology. 456(2) : 393-405, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18043943
  • Stocker, PJ.Bennett, ES. Differential sialylation modulates voltage-gated Na+ channel gating throughout the developing myocardium. The Journal of general physiology. 127(3) : 253-65, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16476705
  • Johnson, D.Bennett, ES. Isoform-specific effects of the beta2 subunit on voltage-gated sodium channel gating. The Journal of biological chemistry. 281(36) : 25875-81, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16847056
  • Bennett, ES. Channel activation voltage alone is directly altered in an isoform-specific manner by Na(v1.4) and Na(v1.5) cytoplasmic linkers. The Journal of membrane biology. 197(3) : 155-68, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15042347
  • Bennett, ES.Smith, BA.Harper, JM. Voltage-gated Na+ channels confer invasive properties on human prostate cancer cells. Pflügers Archiv : European journal of physiology. 447(6) : 908-14, 2004. http://www.ncbi.nlm.nih.gov/pubmed/14677067
  • Johnson, D.Montpetit, ML.Stocker, PJ.Bennett, ES. The sialic acid component of the beta1 subunit modulates voltage-gated sodium channel function. The Journal of biological chemistry. 279(43) : 44303-10, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15316006
  • Bennett, ES. Isoform-specific effects of sialic acid on voltage-dependent Na+ channel gating: functional sialic acids are localized to the S5-S6 loop of domain I. The Journal of physiology. 538(Pt 3) : 675-90, 2002. http://www.ncbi.nlm.nih.gov/pubmed/11826157
  • Bennett, ES. Channel cytoplasmic loops alter voltage-dependent sodium channel activation in an isoform-specific manner. The Journal of physiology. 535(Pt 2) : 371-81, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11533130
  • Bennett, ES. Effects of channel cytoplasmic regions on the activation mechanisms of cardiac versus skeletal muscle Na(+) channels. Biophysical journal. 77(6) : 2999-3009, 1999. http://www.ncbi.nlm.nih.gov/pubmed/10585922

Positions Held

  • Associate Professor (College of Medicine, University of South Florida 2002 - 2008)
  • Assistant Professor (College of Medicine, University of South Florida 1997 - 2002)
  • Instructor Fellow (Health Sciences Center, University of Colorado 1994 - 1997)
  • Postdoctoral Fellow in Physiology (Biophysicsm and Neuroscience, Health Sciences Center, University of Colorado 1992 - 1994)
  • Graduate Fellow University of Rochester 1986 - 1991)
  • Engineer Lincoln Electric Company 1985 - 1986)
  • Engineering Co-op (semiconductor department, General Electric Company 1983 - 1984)

Awards/Honors

  • Class of 2015, Outstanding Freshman Instructor Award (University of South Florida, Morsani College of Medicine - 2013)
  • Robert J. Grasso Award, for outstanding dedication to graduate education (University of South Florida College of Medicine - 2008)
  • Robert J. Grasso Award, for outstanding dedication to graduate education (University of South Florida Colelge of medicine - 2004)
  • Presidents Award for Faculty Excellence (University of South Florida - 2003)
  • Most Outstanding Preclinical Professor Award (University of South Florida College of Medicine - 2003)
  • Outstanding Contribution to the Program in Undergraduate Medical Education Award (University of South Florida, College of Medicine - 2001)
  • Wallace O. Fenn Award (most outstanding dissertation) (University of Rochester School of Medicine and Dentistry - 1992)
  • Proctor & Gamble Student Award in Cell and General Physiology (American Physiological Society - 1991)
  • William F. Neuman Award (Deptartment of Biophysics, University of Rochester, School of Medicine and Denstistry - 1990)