CraigDoupnik

Craig Doupnik, Ph.D.

Associate Professor, College Of Medicine Molecular Pharmacology & Physiology

College Of Medicine Molecular Pharmacology & Physiology

Contact Info 12901 Bruce B. Downs Blvd.
MDC 8
Tampa, FL 33612

Academic Email: cdoupnik@health.usf.edu

Academic Phone: (813) 974-1557

Education

  • PHD, Physiology & Biophysics, University Of Cincinnati, 1993
  • M.Sc., Physiology & Biophysics, University of Cincinnati, 1989
  • B.Sc., Biological Sciences, Ohio State University, 1984

Interdisciplinary and Emerging Signature Programs

  • Biomedical Engineering & Nanomedicine
  • Cancer Biology
  • Cardiovascular Sciences
  • Cellular and Molecular Biology
  • Neuroscience

Research Interests

  • • Ion channel regulation by G protein signaling networks
  • • Computational biology & protein engineering

Recent Publications

  • Doupnik, CA. GPCR-Kir channel signaling complexes: defining rules of engagement. Journal of receptor and signal transduction research. 28(1-2) : 83-91, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18437632
  • Drenan, RM.Doupnik, CA.Jayaraman, M.Buchwalter, AL.Kaltenbronn, KM.Huettner, JE.Linder, ME.Blumer, KJ. R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism. The Journal of biological chemistry. 281(38) : 28222-31, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16867977
  • Jaén, C.Doupnik, CA. RGS3 and RGS4 differentially associate with G protein-coupled receptor-Kir3 channel signaling complexes revealing two modes of RGS modulation. Precoupling and collision coupling. The Journal of biological chemistry. 281(45) : 34549-60, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16973624
  • Drenan, RM.Doupnik, CA.Boyle, MP.Muglia, LJ.Huettner, JE.Linder, ME.Blumer, KJ. Palmitoylation regulates plasma membrane-nuclear shuttling of R7BP, a novel membrane anchor for the RGS7 family. The Journal of cell biology. 169(4) : 623-33, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15897264
  • Jaén, C.Doupnik, CA. Neuronal Kir3.1/Kir3.2a channels coupled to serotonin 1A and muscarinic m2 receptors are differentially modulated by the "short" RGS3 isoform. Neuropharmacology. 49(4) : 465-76, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15935408
  • Doupnik, CA.Jaén, C.Zhang, Q. Measuring the modulatory effects of RGS proteins on GIRK channels. Methods in enzymology. 389: 131-54, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15313564
  • Zhang, Q.Dickson, A.Doupnik, CA. Gbetagamma-activated inwardly rectifying K(+) (GIRK) channel activation kinetics via Galphai and Galphao-coupled receptors are determined by Galpha-specific interdomain interactions that affect GDP release rates. The Journal of biological chemistry. 279(28) : 29787-96, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15123672
  • Zhang, Q.Pacheco, MA.Doupnik, CA. Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation. The Journal of physiology. 545(Pt 2) : 355-73, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12456817
  • Doupnik, CA.Xu, T.Shinaman, JM. Profile of RGS expression in single rat atrial myocytes. Biochimica et biophysica acta. 1522(2) : 97-107, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11750060
  • Cunnick, JM.Mei, L.Doupnik, CA.Wu, J. Phosphotyrosines 627 and 659 of Gab1 constitute a bisphosphoryl tyrosine-based activation motif (BTAM) conferring binding and activation of SHP2. The Journal of biological chemistry. 276(26) : 24380-7, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11323411
  • Ehrengruber, MU.Doupnik, CA.Xu, Y.Garvey, J.Jasek, MC.Lester, HA.Davidson, N. Activation of heteromeric G protein-gated inward rectifier K+ channels overexpressed by adenovirus gene transfer inhibits the excitability of hippocampal neurons. Proceedings of the National Academy of Sciences of the United States of America. 94(13) : 7070-5, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9192693
  • Doupnik, CA.Davidson, N.Lester, HA.Kofuji, P. RGS proteins reconstitute the rapid gating kinetics of gbetagamma-activated inwardly rectifying K+ channels. Proceedings of the National Academy of Sciences of the United States of America. 94(19) : 10461-6, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9294233
  • Kofuji, P.Doupnik, CA.Davidson, N.Lester, HA. A unique P-region residue is required for slow voltage-dependent gating of a G protein-activated inward rectifier K+ channel expressed in Xenopus oocytes. The Journal of physiology. 490 ( Pt 3): 633-45, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8683463
  • Doupnik, CA.Dessauer, CW.Slepak, VZ.Gilman, AG.Davidson, N.Lester, HA. Time resolved kinetics of direct G beta 1 gamma 2 interactions with the carboxyl terminus of Kir3.4 inward rectifier K+ channel subunits. Neuropharmacology. 35(7) : 923-31, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8938723
  • Dascal, N.Doupnik, CA.Ivanina, T.Bausch, S.Wang, W.Lin, C.Garvey, J.Chavkin, C.Lester, HA.Davidson, N. Inhibition of function in Xenopus oocytes of the inwardly rectifying G-protein-activated atrial K channel (GIRK1) by overexpression of a membrane-attached form of the C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America. 92(15) : 6758-62, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7542774
  • Doupnik, CA.Lim, NF.Kofuji, P.Davidson, N.Lester, HA. Intrinsic gating properties of a cloned G protein-activated inward rectifier K+ channel. The Journal of general physiology. 106(1) : 1-23, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7494135
  • Doupnik, CA.Davidson, N.Lester, HA. The inward rectifier potassium channel family. Current opinion in neurobiology. 5(3) : 268-77, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7580148
  • Doupnik, CA.Pun, RY. G-protein activation mediates prepulse facilitation of Ca2+ channel currents in bovine chromaffin cells. The Journal of membrane biology. 140(1) : 47-56, 1994. http://www.ncbi.nlm.nih.gov/pubmed/8051693
  • Doupnik, CA.Pun, RY. Cyclic AMP-dependent phosphorylation modifies the gating properties of L-type Ca2+ channels in bovine adrenal chromaffin cells. Pflügers Archiv : European journal of physiology. 420(1) : 61-71, 1992. http://www.ncbi.nlm.nih.gov/pubmed/1313168
  • Plews, PI.Abdel-Malek, ZA.Doupnik, CA.Leikauf, GD. Endothelin stimulates chloride secretion across canine tracheal epithelium. The American journal of physiology. 261(2 Pt 1) : L188-94, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1651666
  • Lee, HK.Ohya, Y.Doupnik, CA.Sperelakis, N. Effects of azelastine on contraction of guinea pig tracheal smooth muscle. European journal of pharmacology. 187(1) : 67-74, 1990. http://www.ncbi.nlm.nih.gov/pubmed/2272354
  • Doupnik, CA.Leikauf, GD. Acrolein stimulates eicosanoid release from bovine airway epithelial cells. The American journal of physiology. 259(4 Pt 1) : L222-9, 1990. http://www.ncbi.nlm.nih.gov/pubmed/2221083
  • Leikauf, GD.Claesson, HE.Doupnik, CA.Hybbinette, S.Grafström, RC. Cysteinyl leukotrienes enhance growth of human airway epithelial cells. The American journal of physiology. 259(4 Pt 1) : L255-61, 1990. http://www.ncbi.nlm.nih.gov/pubmed/2171354
  • Murlas, CG.Doupnik, CA. Electromechanical coupling of ferret airway smooth muscle in response to leukotriene C4. Journal of applied physiology (Bethesda, Md. : 1985). 66(6) : 2533-8, 1989. http://www.ncbi.nlm.nih.gov/pubmed/2745315
  • Leikauf, GD.Doupnik, CA.Leming, LM.Wey, HE. Sulfidopeptide leukotrienes mediate acrolein-induced bronchial hyperresponsiveness. Journal of applied physiology (Bethesda, Md. : 1985). 66(4) : 1838-45, 1989. http://www.ncbi.nlm.nih.gov/pubmed/2543658
  • Leikauf, GD.Leming, LM.O'Donnell, JR.Doupnik, CA. Bronchial responsiveness and inflammation in guinea pigs exposed to acrolein. Journal of applied physiology (Bethesda, Md. : 1985). 66(1) : 171-8, 1989. http://www.ncbi.nlm.nih.gov/pubmed/2917919

Positions Held

  • Assistant Professor (Department of Physiology & Biophysics, College of Medicine, University of South Florida 1997 - 2003)
  • Postdoctoral Fellow (Biology, California Institute of Technology 1993 - 1997)

Awards/Honors

  • Dean's Recognition Award for Teaching Excellence (University of South Florida College of Medicine - 2013)
  • Robert J. Grasso Award (Association of Medical Science Graduate Students (AMSGS) - 2003)
  • Robert J. Boucek Research Award (American Heart Association - Florida & Puerto Rico Affiliate - 1998)
  • Initial Investigator Award (American Heart Association - Florida & Puerto Rico Affiliate - 1998)
  • Postdoctoral Fellowship Awards (American Heart Association - Greater Los Angeles Affiliate - 1994)
  • Jeffery Doane Memorial Award (University of Cincinnati College of Medicine - 1991)