ThomasKlein

Thomas Klein, Ph.D.

Professor, College Of Medicine Molecular Medicine

College Of Medicine Molecular Medicine

Professor, Interdisciplinary Oncology, College of Medicine, University of South Florida

Emeritus Professor, Molecular Medicine, USF College of Medicine

Professor, Molecular Medicine, USF College of Medicine

Contact Info Molecular Medicine, Box 7
12901 Bruce Downs Blvd. MDC07
Tampa, FL 33612

Academic Email: tklein@health.usf.edu

Academic Phone: (813) 974-2502

View My C.V.

Education

  • PHD, Medical Microbiology, Creighton University, 1973
  • BS, Biology, Creighton University, 1966

Interdisciplinary and Emerging Signature Programs

  • Allergy, Immunology & Infectious Disease

Research Interests

  • Cannabinoid-based drugs are currently being tested for use as therapeutic agents in the treatment of chronic inflammatory conditions such as multiple sclerosis, arthritis, inflammatory pain, and Alzheimers disease. It is our goal to discover what effect the drugs have on the immune system and resistance to infections and tumors. Currently, we are studying the distribution, function, and molecular biology of cannabinoid receptors on immune cells as well as the effect of these drugs on resistance to infection.
  • In addition to our work with cannabinoids and immunity, we are also studying immune mechanisms important in vaccine development. It is becoming clear that effective public health measures to combat emerging infectious diseases must include a good vaccine program. The development of useful vaccines relies on a thorough understanding of the mechanisms of immunity to microbes. Therefore, we are investigating the cellular and molecular mechanisms involved in the regulation of immune T helper cell activity important in fighting infections. One model system we are studying intensively involves the cellular immune mechanisms induced by infection with the microbe that causes Legionnaires' Disease; a fatal pneumonia of concern in immunosuppressed populations and other select populations world-wide.

Memberships

  • Society on Neuroimmune Pharmacology 2002 - Present)
  • American Association of Immunologists 1990 - Present)
  • International Cannabinoid Research Society 1990 - Present)
  • American Society for Microbiology 1973 - Present)

Recent Publications

  • Newton, CA.Klein, TW. Cannabinoid 2 (CB2) Receptor Involvement in the Down-regulation but not Up-regulation of Serum IgE Levels in Immunized Mice. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. , 2012. http://www.ncbi.nlm.nih.gov/pubmed/22552780
  • Lengacher, CA.Kip, KE.Post-White, J.Fitzgerald, S.Newton, C.Barta, M.Jacobsen, PB.Shelton, MM.Moscoso, M.Johnson-Mallard, V.Harris, E.Loftus, L.Cox, C.Le, N.Goodman, M.Djeu, J.Widen, RH.Bercu, BB.Klein, TW. Lymphocyte Recovery After Breast Cancer Treatment and Mindfulness-Based Stress Reduction (MBSR) Therapy. Biological research for nursing. , 2011. http://www.ncbi.nlm.nih.gov/pubmed/22084404
  • Sherwood, TA.Nong, L.Agudelo, M.Newton, C.Widen, R.Klein, TW. Identification of transcription start sites and preferential expression of select CB2 transcripts in mouse and human B lymphocytes. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 4(4) : 476-88, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19757078
  • Lengacher, CA.Johnson-Mallard, V.Post-White, J.Moscoso, MS.Jacobsen, PB.Klein, TW.Widen, RH.Fitzgerald, SG.Shelton, MM.Barta, M.Goodman, M.Cox, CE.Kip, KE. Randomized controlled trial of mindfulness-based stress reduction (MBSR) for survivors of breast cancer. Psycho-oncology. 18(12) : 1261-72, 2009. http://www.ncbi.nlm.nih.gov/pubmed/19235193
  • Newton, CA.Chou, PJ.Perkins, I.Klein, TW. CB(1) and CB(2) cannabinoid receptors mediate different aspects of delta-9-tetrahydrocannabinol (THC)-induced T helper cell shift following immune activation by Legionella pneumophila infection. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 4(1) : 92-102, 2009. http://www.ncbi.nlm.nih.gov/pubmed/18792785
  • Chou, PJ.Newton, CA.Perkins, I.Friedman, H.Klein, TW. Suppression of dendritic cell activation by anthrax lethal toxin and edema toxin depends on multiple factors including cell source, stimulus used, and function tested. DNA and cell biology. 27(12) : 637-48, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18821847
  • Agudelo, M.Newton, C.Widen, R.Sherwood, T.Nong, L.Friedman, H.Klein, TW. Cannabinoid receptor 2 (CB2) mediates immunoglobulin class switching from IgM to IgE in cultures of murine-purified B lymphocytes. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 3(1) : 35-42, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18247126
  • Klein, TW.Peterson, P.Cabral, G.Kaminski, N.Adler, M. In memory of Billy R. Martin (1943-2008). Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 3(4) : 201-2, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18720014
  • Newton, CA.Perkins, I.Widen, RH.Friedman, H.Klein, TW. Role of Toll-like receptor 9 in Legionella pneumophila-induced interleukin-12 p40 production in bone marrow-derived dendritic cells and macrophages from permissive and nonpermissive mice. Infection and immunity. 75(1) : 146-51, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17060467
  • Mamata, Y.Hakki, A.Newton, C.Burdash, N.Klein, TW.Friedman, H. Differential effects of Chlamydia pneumoniae infection on cytokine levels in human T lymphocyte- and monocyte-derived cell cultures. International journal of medical microbiology : IJMM. 297(2) : 109-15, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17280867
  • Rogers, J.Hakki, A.Perkins, I.Newton, C.Widen, R.Burdash, N.Klein, T.Friedman, H. Legionella pneumophila infection up-regulates dendritic cell Toll-like receptor 2 (TLR2)/TLR4 expression and key maturation markers. Infection and immunity. 75(6) : 3205-8, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17371856
  • Klein, TW.Cabral, GA. Cannabinoid-induced immune suppression and modulation of antigen-presenting cells. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 1(1) : 50-64, 2006. http://www.ncbi.nlm.nih.gov/pubmed/18040791
  • Lu, T.Newton, C.Perkins, I.Friedman, H.Klein, TW. Cannabinoid treatment suppresses the T-helper cell-polarizing function of mouse dendritic cells stimulated with Legionella pneumophila infection. The Journal of pharmacology and experimental therapeutics. 319(1) : 269-76, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16837556
  • Friedman, H.Pross, S.Klein, TW. Addictive drugs and their relationship with infectious diseases. FEMS immunology and medical microbiology. 47(3) : 330-42, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16872369
  • Lu, T.Newton, C.Perkins, I.Friedman, H.Klein, TW. Role of cannabinoid receptors in Delta-9-tetrahydrocannabinol suppression of IL-12p40 in mouse bone marrow-derived dendritic cells infected with Legionella pneumophila. European journal of pharmacology. 532(1-2) : 170-7, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16443217
  • Klein, TW. Cannabinoid-based drugs as anti-inflammatory therapeutics. Nature reviews. Immunology. 5(5) : 400-11, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15864274
  • Rogers, J.Perkins, I.van Olphen, A.Burdash, N.Klein, TW.Friedman, H. Epigallocatechin gallate modulates cytokine production by bone marrow-derived dendritic cells stimulated with lipopolysaccharide or muramyldipeptide, or infected with Legionella pneumophila. Experimental biology and medicine (Maywood, N.J.). 230(9) : 645-51, 2005. http://www.ncbi.nlm.nih.gov/pubmed/16179732
  • Mamata, Y.Hakki, A.Yamamoto, Y.Newton, C.Klein, TW.Pross, S.Friedman, H. Nicotine modulates cytokine production by Chlamydia pneumoniae infected human peripheral blood cells. International immunopharmacology. 5(4) : 749-56, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15710343
  • Ehrhart, J.Obregon, D.Mori, T.Hou, H.Sun, N.Bai, Y.Klein, T.Fernandez, F.Tan, J.Shytle, RD. Stimulation of cannabinoid receptor 2 (CB2) suppresses microglial activation. Journal of neuroinflammation. 2: 29, 2005. http://www.ncbi.nlm.nih.gov/pubmed/16343349
  • Newton, CA.Lu, T.Nazian, SJ.Perkins, I.Friedman, H.Klein, TW. The THC-induced suppression of Th1 polarization in response to Legionella pneumophila infection is not mediated by increases in corticosterone and PGE2. Journal of leukocyte biology. 76(4) : 854-61, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15258190
  • Medveczky, MM.Sherwood, TA.Klein, TW.Friedman, H.Medveczky, PG. Delta-9 tetrahydrocannabinol (THC) inhibits lytic replication of gamma oncogenic herpesviruses in vitro. BMC medicine. 2: 34, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15369590
  • Klein, TW.Newton, C.Larsen, K.Chou, J.Perkins, I.Lu, L.Nong, L.Friedman, H. Cannabinoid receptors and T helper cells. Journal of neuroimmunology. 147(1-2) : 91-4, 2004. http://www.ncbi.nlm.nih.gov/pubmed/14741435
  • Klein, TW.Newton, C.Larsen, K.Lu, L.Perkins, I.Nong, L.Friedman, H. The cannabinoid system and immune modulation. Journal of leukocyte biology. 74(4) : 486-96, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12960289
  • Friedman, H.Newton, C.Klein, TW. Microbial infections, immunomodulation, and drugs of abuse. Clinical microbiology reviews. 16(2) : 209-19, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12692094
  • Matsunaga, K.Yamaguchi, H.Klein, TW.Friedman, H.Yamamoto, Y. Legionella pneumophila suppresses macrophage interleukin-12 production by activating the p42/44 mitogen-activated protein kinase cascade. Infection and immunity. 71(11) : 6672-5, 2003. http://www.ncbi.nlm.nih.gov/pubmed/14573695
  • Nong, L.Newton, C.Cheng, Q.Friedman, H.Roth, MD.Klein, TW. Altered cannabinoid receptor mRNA expression in peripheral blood mononuclear cells from marijuana smokers. Journal of neuroimmunology. 127(1-2) : 169-76, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12044989
  • Town, T.Vendrame, M.Patel, A.Poetter, D.DelleDonne, A.Mori, T.Smeed, R.Crawford, F.Klein, T.Tan, J.Mullan, M. Reduced Th1 and enhanced Th2 immunity after immunization with Alzheimer's beta-amyloid(1-42). Journal of neuroimmunology. 132(1-2) : 49-59, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12417433
  • Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. In vitro therapeutic effect of epigallocatechin gallate on nicotine-induced impairment of resistance to Legionella pneumophila infection of established MH-S alveolar macrophages. The Journal of infectious diseases. 185(2) : 229-36, 2002. http://www.ncbi.nlm.nih.gov/pubmed/11807697
  • Friedman, H.Yamamoto, Y.Klein, TW. Legionella pneumophila pathogenesis and immunity. Seminars in pediatric infectious diseases. 13(4) : 273-9, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12491233
  • Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. Epigallocatechin gallate, a potential immunomodulatory agent of tea components, diminishes cigarette smoke condensate-induced suppression of anti-Legionella pneumophila activity and cytokine responses of alveolar macrophages. Clinical and diagnostic laboratory immunology. 9(4) : 864-71, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12093687
  • Resto-Ruiz, SI.Schmiederer, M.Sweger, D.Newton, C.Klein, TW.Friedman, H.Anderson, BE. Induction of a potential paracrine angiogenic loop between human THP-1 macrophages and human microvascular endothelial cells during Bartonella henselae infection. Infection and immunity. 70(8) : 4564-70, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12117969
  • Lee, SF.Newton, C.Widen, R.Friedman, H.Klein, TW. Differential expression of cannabinoid CB(2) receptor mRNA in mouse immune cell subpopulations and following B cell stimulation. European journal of pharmacology. 423(2-3) : 235-41, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11448490
  • Klein, TW.Newton, CA.Friedman, H. Cannabinoids and the immune system. Pain research & management : the journal of the Canadian Pain Society = journal de la société canadienne pour le traitement de la douleur. 6(2) : 95-101, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11854771
  • Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. Legionella pneumophila replication in macrophages inhibited by selective immunomodulatory effects on cytokine formation by epigallocatechin gallate, a major form of tea catechins. Infection and immunity. 69(6) : 3947-53, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11349063
  • Salins, S.Newton, C.Widen, R.Klein, TW.Friedman, H. Differential induction of gamma interferon in Legionella pneumophila-infected macrophages from BALB/c and A/J mice. Infection and immunity. 69(6) : 3605-10, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11349020
  • Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. Involvement of nicotinic acetylcholine receptors in suppression of antimicrobial activity and cytokine responses of alveolar macrophages to Legionella pneumophila infection by nicotine. Journal of immunology (Baltimore, Md. : 1950). 167(11) : 6518-24, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11714820
  • Matsunaga, K.Klein, TW.Newton, C.Friedman, H.Yamamoto, Y. Legionella pneumophila suppresses interleukin-12 production by macrophages. Infection and immunity. 69(3) : 1929-33, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11179377
  • Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. Alveolar macrophage cell Line MH-S is valuable as an in vitro model for Legionella pneumophila infection. American journal of respiratory cell and molecular biology. 24(3) : 326-31, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11245632
  • Town, T.Tan, J.Sansone, N.Obregon, D.Klein, T.Mullan, M. Characterization of murine immunoglobulin G antibodies against human amyloid-beta1-42. Neuroscience letters. 307(2) : 101-4, 2001. http://www.ncbi.nlm.nih.gov/pubmed/11427310
  • McHugh, SL.Newton, CA.Yamamoto, Y.Klein, TW.Friedman, H. Tumor necrosis factor induces resistance of macrophages to Legionella pneumophila infection. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 224(3) : 191-6, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10865236
  • Nakachi, N.Klein, TW.Friedman, H.Yamamoto, Y. Helicobacter pylori infection of human gastric epithelial cells induces IL-8 and TNFalpha, but not TGFbeta1 mRNA. FEMS immunology and medical microbiology. 29(1) : 23-6, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10967256
  • Newton, C.McHugh, S.Widen, R.Nakachi, N.Klein, T.Friedman, H. Induction of interleukin-4 (IL-4) by legionella pneumophila infection in BALB/c mice and regulation of tumor necrosis factor alpha, IL-6, and IL-1beta. Infection and immunity. 68(9) : 5234-40, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10948149
  • McHugh, S.Yamamoto, Y.Klein, TW.Friedman, H. Differential expression of IL-1 and TNF receptors in murine macrophages infected with virulent vs. avirulent Legionella pneumophila. Canadian journal of microbiology. 46(10) : 885-91, 2000. http://www.ncbi.nlm.nih.gov/pubmed/11068674
  • Klein, TW.Newton, CA.Nakachi, N.Friedman, H. Delta 9-tetrahydrocannabinol treatment suppresses immunity and early IFN-gamma, IL-12, and IL-12 receptor beta 2 responses to Legionella pneumophila infection. Journal of immunology (Baltimore, Md. : 1950). 164(12) : 6461-6, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10843702
  • Noe, SN.Newton, C.Widen, R.Friedman, H.Klein, TW. Anti-CD40, anti-CD3, and IL-2 stimulation induce contrasting changes in CB1 mRNA expression in mouse splenocytes. Journal of neuroimmunology. 110(1-2) : 161-7, 2000. http://www.ncbi.nlm.nih.gov/pubmed/11024546
  • Klein, TW.Lane, B.Newton, CA.Friedman, H. The cannabinoid system and cytokine network. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 225(1) : 1-8, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10998193
  • McHugh, SL.Yamamoto, Y.Klein, TW.Friedman, H. Murine macrophages differentially produce proinflammatory cytokines after infection with virulent vs. avirulent Legionella pneumophila. Journal of leukocyte biology. 67(6) : 863-8, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10857860
  • Nakachi, N.Matsunaga, K.Klein, TW.Friedman, H.Yamamoto, Y. Differential effects of virulent versus avirulent Legionella pneumophila on chemokine gene expression in murine alveolar macrophages determined by cDNA expression array technique. Infection and immunity. 68(10) : 6069-72, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10992525

Positions Held

  • Interim Chair (Medical Microbiology and Immunology, USF College of Medicine 2003 - 2006)
  • Professor (Medical Microbiology and Immunology, College of Medicine, University of South Florida 1990 - 2006)
  • Vice Chair (Medical Microbiology and Immunology, USF College of Medicine 1989 - 2002)
  • Associate Professor (Medical Microbiology and Immunology, College of Medicine, University of South Florida 1984 - 1990)
  • Assistant Professor (Medical Microbiology and Immunology, College of Medicine, University of South Florida 1976 - 1983)
  • Postdoc (Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida 1973 - 1975)
  • Teaching Assistant (Medical Microbiology, College of Medicine, Creighton University 1968 - 1972)

Awards/Honors

  • Wybran Award (Society on Neuroimmune Pharmacology - 2008)
  • USF Million Dollar Researcher Award (University of South Florida - 2006)
  • President's Award for Faculty Excellence (University of South Florida - 2003)
  • Distingushed Scholar Award (Theodore and Venette Askounes-Ashford - 2002)
  • Professorial Excellence Award Recipient (State of Florida - 1999)
  • Alumni Merit Award (Creighton University - 1996)
  • Robert J. Grasso Award for Graduate Education (AMSGS - 1996)