RobertDeschenes

Robert Deschenes, Ph.D.

Chair, College Of Medicine Molecular Medicine

Contact Info 12901 Bruce B. Downs Blvd.
MDC 7
Tampa, FL 33612

Academic Email: rdeschen@health.usf.edu

Academic Phone: (813) 974-6393

View My C.V.

Education

  • PHD, Biochemistry, Purdue University, 1984
  • M.S., Biochemistry, Tufts University, 1980
  • B.A., Biology, St. Michael's College, 1977

Interdisciplinary and Emerging Signature Programs

  • Cancer Biology

Research Interests

  • The Deschenes research group and collaborators are currently focused in two general areas. The first deals with the posttranslational modification and the subcellular localization of cancer signal transduction proteins. The second deals with stress signaling and the characterization of histidine kinases.

Memberships

  • AAAS (Fellow, 2013 - Present)
  • ASBMB (Member, 1990 - Present)

Recent Publications

  • Hamel, LD.Deschenes, RJ.Mitchell, DA. A fluorescence-based assay to monitor autopalmitoylation of zDHHC proteins applicable to high-throughput screening. Analytical biochemistry. 460: 1-8, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24878334
  • Mitchell, DA.Hamel, LD.Reddy, KD.Farh, L.Rettew, LM.Sanchez, PR.Deschenes, RJ. Mutations in the X-linked intellectual disability gene, zDHHC9, alter autopalmitoylation activity by distinct mechanisms. The Journal of biological chemistry. 289(26) : 18582-92, 2014. http://www.ncbi.nlm.nih.gov/pubmed/24811172
  • Luongo, K.Holton, A.Kaushik, A.Spence, P.Ng, B.Deschenes, R.Sundaram, S.Bhansali, S. Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy. Biomicrofluidics. 7(3) : 34108, 2013. http://www.ncbi.nlm.nih.gov/pubmed/24404028
  • Mitchell, DA.Hamel, LD.Ishizuka, K.Mitchell, G.Schaefer, LM.Deschenes, RJ. The Erf4 subunit of the yeast Ras palmitoyl acyltransferase is required for stability of the Acyl-Erf2 intermediate and palmitoyl transfer to a Ras2 substrate. The Journal of biological chemistry. 287(41) : 34337-48, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22904317
  • Pedram, A.Razandi, M.Deschenes, RJ.Levin, ER. DHHC-7 and -21 are palmitoylacyltransferases for sex steroid receptors. Molecular biology of the cell. 23(1) : 188-99, 2012. http://www.ncbi.nlm.nih.gov/pubmed/22031296
  • Mitchell, DA.Mitchell, G.Ling, Y.Budde, C.Deschenes, RJ. Mutational analysis of Saccharomyces cerevisiae Erf2 reveals a two-step reaction mechanism for protein palmitoylation by DHHC enzymes. The Journal of biological chemistry. 285(49) : 38104-14, 2010. http://www.ncbi.nlm.nih.gov/pubmed/20851885
  • Vinnakota, KC.Mitchell, DA.Deschenes, RJ.Wakatsuki, T.Beard, DA. Analysis of the diffusion of Ras2 in Saccharomyces cerevisiae using fluorescence recovery after photobleaching. Physical biology. 7(2) : 026011, 2010. http://www.ncbi.nlm.nih.gov/pubmed/20526029
  • Jennings, BC.Nadolski, MJ.Ling, Y.Baker, MB.Harrison, ML.Deschenes, RJ.Linder, ME. 2-bromopalmitate and 2-(2-Hydroxy-5-nitro-benzylidene)-benzo[b]thiophen-3-one inhibit DHHC-mediated palmitoylation in vitro. Journal of lipid research. , 2008. http://www.ncbi.nlm.nih.gov/pubmed/18827284
  • Shankarnarayan, S.Narang, SS.Malone, CL.Deschenes, RJ.Fassler, JS. Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein. The Journal of biological chemistry. 283(4) : 1962-73, 2008. http://www.ncbi.nlm.nih.gov/pubmed/18048366
  • Linder, ME.Deschenes, RJ. Palmitoylation: policing protein stability and traffic. Nature reviews. Molecular cell biology. 8(1) : 74-84, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17183362
  • Budde, C.Schoenfish, MJ.Linder, ME.Deschenes, RJ. Purification and characterization of recombinant protein acyltransferases. Methods (San Diego, Calif.). 40(2) : 143-50, 2006. http://www.ncbi.nlm.nih.gov/pubmed/17012026
  • Mitchell, DA.Vasudevan, A.Linder, ME.Deschenes, RJ. Protein palmitoylation by a family of DHHC protein S-acyltransferases. Journal of lipid research. 47(6) : 1118-27, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16582420
  • Wang, G.Deschenes, RJ. Plasma membrane localization of Ras requires class C Vps proteins and functional mitochondria in Saccharomyces cerevisiae. Molecular and cellular biology. 26(8) : 3243-55, 2006. http://www.ncbi.nlm.nih.gov/pubmed/16581797
  • Swarthout, JT.Lobo, S.Farh, L.Croke, MR.Greentree, WK.Deschenes, RJ.Linder, ME. DHHC9 and GCP16 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras. The Journal of biological chemistry. 280(35) : 31141-8, 2005. http://www.ncbi.nlm.nih.gov/pubmed/16000296
  • Linder, ME.Deschenes, RJ. Model organisms lead the way to protein palmitoyltransferases. Journal of cell science. 117(Pt 4) : 521-6, 2004. http://www.ncbi.nlm.nih.gov/pubmed/14730009
  • Dong, X.Mitchell, DA.Lobo, S.Zhao, L.Bartels, DJ.Deschenes, RJ. Palmitoylation and plasma membrane localization of Ras2p by a nonclassical trafficking pathway in Saccharomyces cerevisiae. Molecular and cellular biology. 23(18) : 6574-84, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12944483
  • Linder, ME.Deschenes, RJ. New insights into the mechanisms of protein palmitoylation. Biochemistry. 42(15) : 4311-20, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12693927
  • Lobo, S.Greentree, WK.Linder, ME.Deschenes, RJ. Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae. The Journal of biological chemistry. 277(43) : 41268-73, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12193598
  • Zhan, XL.Hong, Y.Zhu, T.Mitchell, AP.Deschenes, RJ.Guan, KL. Essential functions of protein tyrosine phosphatases PTP2 and PTP3 and RIM11 tyrosine phosphorylation in Saccharomyces cerevisiae meiosis and sporulation. Molecular biology of the cell. 11(2) : 663-76, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10679022

Positions Held

  • Professor and Chairman (Department of Biochemistry, Joseph F. Heil, Jr. Professor of Molecular Oncogenesis, Medical College of Wisconsin 2004 - 2008)
  • Director of Research (Cancer Center, Medical College of Wisconsin 2007 - 2008)
  • Professor (Department of Biochemistry, University of Iowa 2000 - 2004)
  • Director (Genetics Interdisciplinary Program, University of Iowa 1999 - 2004)
  • Vice Chair (Department of Biochemistry, University of Iowa 2002 - 2004)
  • Associate Professor (Department of Biochemistry, University of Iowa 1995 - 2000)
  • Assistant Professor (Department of Biochemistry, University of Iowa 1989 - 1994)
  • Postdoctoral Fellow (Department of Molecular Biology, Princeton University 1984 - 1989)

Awards/Honors

  • Basil O'Connor Scholar (1989-1992) (March of Dimes - 1987)
  • Postdoctoral Research Fellow (1987-1989) (National Institutes of Health - 1987)
  • Postdoctoral Research Fellow (1985-1987) (Anna Fuller Cancer Fund - 1985)
  • Fellowship (American Diabetes Association Summer Research Fellow - 1983)
  • Fellowship (1981-1983) (David Ross Research Fellow - 1981)