Andreas Seyfang, Ph.D.
Associate Professor, College Of Medicine Molecular Medicine
College Of Medicine Molecular Medicine
Associate Professor (joint appointment), Department of Internal Medicine- Division of Infectious Diseases, College of Medicine, University of South Florida
Associate Professor (joint appointment), Department of Global Health, College of Public Health, University of South Florida
Associate Professor (joint appointment), School of Physical Therapy & Rehabilitation Sciences, College of Medicine, University of South Florida
Associate Professor, Department of Molecular Medicine, College of Medicine, University of South Florida
Associate Professor (joint appointment), Department of Neurosurgery, College of Medicine, University of South Florida
Molecular Medicine, MDC Box 7
12901 Bruce B. Downs Blvd.
Tampa, FL 33612
Academic Email: firstname.lastname@example.org
Academic Phone: (813) 974-2332
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- Postdoc, Molecular Microbiology, Oregon Health Sciences University, 1995
- PHD, Biochemistry, University Of Tubingen, 1993
- Postdoc, Molecular Parasitology, University of Bordeaux, 1993
- MSc, Parasitology, Zoology, University of Tübingen, 1989
- DAAD Scholar, Microbiology, Parasitology, Duke University, 1984
- BSc, Biology, University of Tübingen, 1983
Interdisciplinary and Emerging Signature Programs
- Allergy, Immunology & Infectious Disease
- LABORATORY OF MEDICAL MICROBIOLOGY AND MOLECULAR PARASITOLOGY:
Research in the Seyfang laboratory focuses on two major projects (i) membrane permeases (transporters) as target for drug delivery and (ii) cytochrome b5 reductase as enzymatic drug target in opportunistic microbial pathogens including protozoan parasites and nosocomial and neuro-pathogenic fungi.
We are studying membrane permeases (transporters) and receptors in opportunistic microbial pathogens including protozoan parasites (trypanosomes, Leishmania) and nosocomial and neuro-pathogenic fungi (Candida albicans, Cryptococcus neoformans). We use a multi-disciplinary approach of biochemistry, proteomics, molecular pharmacology and genetics to investigate the structure-function relationship, substrate/drug selectivity, protein-protein interaction, and significance for microbial pathogenicity of these membrane proteins at the molecular level in both in vitro culture and animal models. The objective of this project is to use membrane permeases, such as the myo-inositol transporter MIT, for delivery of cytotoxic and parasite-specific substrate analogues as novel pharmacological targets in these microbial pathogens.
A second project investigates cytochrome b5 reductase as enzymatic drug target in these opportunistic pathogens. Cytochrome b5 reductase (Cb5r) plays an important role in P450-mediated detoxification of xenobiotics and drugs, lipid biosynthesis, and the synthesis of cholesterol (humans, Leishmania) or ergosterol (fungi and Trypanosoma cruzi). Hence we use recombinant Cb5r protein for biochemical, structural and pharmacological studies and in silico modeling as a novel pharmacological target in these opportunistic microbial pathogens.
TECHNIQUES that are applied in the laboratory include protein biochemistry and proteomics, microarray and real-time PCR gene expression analysis, site-directed mutagenesis, targeted gene disruption and reverse genetics, heterologeous expression in Xenopus oocytes microinjected with transporter RNA, and the generation of transgenic protozoa and candida cells. Furthermore, we use axenic cultivation of both mammalian and insect forms of trypanosomes and Leishmania, and candida (yeast and hyphae forms) to probe the pharmacology of these important permeases in vivo. Hence, our studies are aimed to develop novel inositol-based drugs such as fluoro-inositol analogues specific for these microbial pathogens.
- American Society for Microbiology (Member, 2008 - Present)
- American Association for the Advancement of Science (Member, 2008 - Present)
- Society for Neuroscience (Member, 2008 - Present)
- American Society for Biochemistry and Molecular Biology (Member, 2008 - Present)
- Seyfang, A., Saporta, S. & Johnson, W.E. Effect of Pre-Medical Education on Student Performance in a First-Year Medical School Curriculum. Medical Science Educator. 23(4) : 712, 2013.
- Seyfang, A., Nazian, S.J., Saporta, S., Doupnik, C.A., Johnson, W.E. & Stevenson, F.T. Assessment of an Adjusted vs. Fixed Pass Line for Student Performance in a Medical School Curriculum. Medical Science Educator. 22(4) : 270-271, 2012.
- Groër, MW.Yolken, RH.Xiao, JC.Beckstead, JW.Fuchs, D.Mohapatra, SS.Seyfang, A.Postolache, TT. Prenatal depression and anxiety in Toxoplasma gondii-positive women. American journal of obstetrics and gynecology. 204(5) : 433.e1-7, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21345406
- Xue, C.Liu, T.Chen, L.Li, W.Liu, I.Kronstad, JW.Seyfang, A.Heitman, J. Role of an expanded inositol transporter repertoire in Cryptococcus neoformans sexual reproduction and virulence. mBio. 1(1) : e00084-10, 2010. http://www.ncbi.nlm.nih.gov/pubmed/20689743
- Liu, L.Bailey, SM.Okuka, M.Muñoz, P.Li, C.Zhou, L.Wu, C.Czerwiec, E.Sandler, L.Seyfang, A.Blasco, MA.Keefe, DL. Telomere lengthening early in development. Nature Cell Biology. 9(12) : 1436-41, 2007. http://www.ncbi.nlm.nih.gov/pubmed/17982445
- Lin, T.Gao, L.Seyfang, A.Oliver, JH. 'Candidatus Borrelia texasensis', from the American dog tick Dermacentor variabilis. International Journal of Systematic and Evolutionary Microbiology. 55(Pt 2) : 685-93, 2005. http://www.ncbi.nlm.nih.gov/pubmed/15774644
- Seyfang, A.Jin, JH. Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round. Analytical Biochemistry. 324(2) : 285-91, 2004. http://www.ncbi.nlm.nih.gov/pubmed/14690693
- Mongan, TP.Ganapasam, S.Hobbs, SB.Seyfang, A. Substrate specificity of the Leishmania donovani myo-inositol transporter: critical role of inositol C-2, C-3 and C-5 hydroxyl groups. Molecular and Biochemical Parasitology. 135(1) : 133-41, 2004. http://www.ncbi.nlm.nih.gov/pubmed/15287594
- Ling, J.Pi, W.Yu, X.Bengra, C.Long, Q.Jin, H.Seyfang, A.Tuan, D. The ERV-9 LTR enhancer is not blocked by the HS5 insulator and synthesizes through the HS5 site non-coding, long RNAs that regulate LTR enhancer function. Nucleic Acids Research. 31(15) : 4582-96, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12888519
- Jin, JH.Seyfang, A. High-affinity myo-inositol transport in Candida albicans: substrate specificity and pharmacology. Microbiology. 149(Pt 12) : 3371-81, 2003. http://www.ncbi.nlm.nih.gov/pubmed/14663071
- Seyfang, A.Landfear, SM. Four conserved cytoplasmic sequence motifs are important for transport function of the Leishmania inositol/H(+) symporter. The Journal of Biological Chemistry. 275(8) : 5687-93, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10681553
- Seyfang, A.Landfear, SM. Substrate depletion upregulates uptake of myo-inositol, glucose and adenosine in Leishmania. Molecular and Biochemical Parasitology. 104(1) : 121-30, 1999. http://www.ncbi.nlm.nih.gov/pubmed/10589986
- Vasudevan, G.Carter, NS.Drew, ME.Beverley, SM.Sanchez, MA.Seyfang, A.Ullman, B.Landfear, SM. Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant. Proceedings of the National Academy of Sciences of the United States of America. 95(17) : 9873-8, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9707568
- Barrett, MP.Tetaud, E.Seyfang, A.Bringaud, F.Baltz, T. Trypanosome glucose transporters. Molecular and Biochemical Parasitology. 91(1) : 195-205, 1998. http://www.ncbi.nlm.nih.gov/pubmed/9574935
- Seyfang, A.Kavanaugh, MP.Landfear, SM. Aspartate 19 and glutamate 121 are critical for transport function of the myo-inositol/H+ symporter from Leishmania donovani. The Journal of Biological Chemistry. 272(39) : 24210-5, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9305873
- Wille, U.Seyfang, A.Duszenko, M. Glucose uptake occurs by facilitated diffusion in procyclic forms of Trypanosoma brucei. European Journal of Biochemistry / FEBS. 236(1) : 228-33, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8617269
- Bakalara, N.Seyfang, A.Davis, C.Baltz, T. Characterization of a life-cycle-stage-regulated membrane protein tyrosine phosphatase in Trypanosoma brucei. European Journal of Biochemistry / FEBS. 234(3) : 871-7, 1995. http://www.ncbi.nlm.nih.gov/pubmed/8575447
- Barrett, MP.Tetaud, E.Seyfang, A.Bringaud, F.Baltz, T. Functional expression and characterization of the Trypanosoma brucei procyclic glucose transporter, THT2. The Biochemical Journal. 312 ( Pt 3): 687-91, 1995. http://www.ncbi.nlm.nih.gov/pubmed/8554506
- Bakalara, N.Seyfang, A.Baltz, T.Davis, C. Trypanosoma brucei and Trypanosoma cruzi: life cycle-regulated protein tyrosine phosphatase activity. Experimental Parasitology. 81(3) : 302-12, 1995. http://www.ncbi.nlm.nih.gov/pubmed/7498427
- Seyfang, A.Duszenko, M. Functional reconstitution of the Trypanosoma brucei plasma-membrane D-glucose transporter. European Journal of Biochemistry / FEBS. 214(2) : 593-7, 1993. http://www.ncbi.nlm.nih.gov/pubmed/8513808
- Duszenko, M. Seyfang, A. Endocytosis and Intracellular Transport of Variant Surface Glycoproteins in Trypanosomes. Advances in Cell and Molecular Biology of Membranes: Membrane Traffic in Protozoa. JAI Press. Greenwich(Plattner, H.; Ed.) : 227-258, 1993.
- Seyfang, A.Duszenko, M. Specificity of glucose transport in Trypanosoma brucei. Effective inhibition by phloretin and cytochalasin B. European Journal of Biochemistry / FEBS. 202(1) : 191-6, 1991. http://www.ncbi.nlm.nih.gov/pubmed/1935976
- Seyfang, A.Mecke, D.Duszenko, M. Degradation, recycling, and shedding of Trypanosoma brucei variant surface glycoprotein. The Journal of Protozoology. 37(6) : 546-52, 1990. http://www.ncbi.nlm.nih.gov/pubmed/2086784
- Frommel, TO.Seyfang, A.Balber, AE. Trypanosoma brucei sspp.: cleavage of variant specific and common glycoproteins during exposure of live cells to trypsin. Experimental Parasitology. 66(2) : 213-24, 1988. http://www.ncbi.nlm.nih.gov/pubmed/2456221
- Robert J. Grasso Award for Outstanding Dedication to Graduate Education (USF College of Medicine - 2009)
- AHA Fellowship (American Heart Association - 1997)
- Alexander von Humboldt Fellow (Alexander von Humboldt Foundation - 1995)
- FRM Fellowship (Fondation pour la Recherche Médicale (France) - 1994)
- PhD, Biochemistry with Magna cum laude (University of Tübingen - 1993)
- EMBO Fellowship (European Molecular Biology Organization - 1993)
- DAAD Fellowship (German Academic Exchange Service - 1984)