Welcome to the website of the USF COM Fred Wright Jr Flow Cytometry Core. Our goal is to provide high quality, friendly service to our users.
For analytical purposes, the Flow Cytometry Core is equipped with two benchtop analyzers from BD Immunocytometry Systems (a 3 laser/8 color BD Canto II and a 4 laser/15 color BD LSR II). These instruments support a large variety of applications, including multicolor cell phenotyping, apoptosis, cell cycle and bead arrays. After orientation and training, the equipment is available for use directly by the investigators. Alternatively experiments can be run and analyzed by the Core Facility staff.
For sorting purposes the facility is equipped with a high speed BD FacsAria IIu sorter (4 laser/11 colors), as well as a magnetic sorter (AutoMacs Pro) from Miltenyi Biotech. The FacsAria IIu is enclosed in a Baker Bioprotect III to support sort of biohazardous agents level 1 and 2.
If you have not used our facility before, please read the How can you use the facility link. If you are not familiar with Flow Cytometry, the “User Education” section on the left side bar will help you. To schedule experiments, please email the facility manager, Charles Szekeres, PhD at firstname.lastname@example.org.