Core Facilities
USF Health - College of Medicine

 
    


GE Typhoon 9410 imager


A microarray slide, spotted with cDNA from Microarray ScoreCard control plate and cDNA from Incyte Genomics, Inc. was hybridized with 25pmol of Cy3 labeled skeletal muscle mRNA and Cy5 labeled liver mRNA. The slide was scanned on Typhoon9410 at 10µm pixel size resolution. (from GE Healthcare)

  • Versatile system handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays.
  • Powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets.
  • Red-, green-, and blue-excitation wavelengths and a wide choice of emission filters enable imaging of an extensive variety of fluorophores.
  • Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.
  • Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
  • Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.

Typhoon 9410 Variable Mode Imager unites proven storage phosphor autoradiography technology with four-color, nonradioactive fluorescent labeling techniques. For DNA, RNA, and protein samples, choose from:

  • Storage phosphor autoradiography
  • Direct blue-excited fluorescence (457, 488 nm)
  • Direct green-excited fluorescence (532 nm)
  • Direct red-excited fluorescence (633 nm)
  • Chemiluminescence

When one of the scanning modes is selected, the appropriate optical components are automatically activated. Typhoon scans gels, blots, and mounted or unmounted storage phosphor screens up to 35 × 43 cm. The instrument exhibits outstanding linearity, quantitative accuracy, and extremely low limits of detection.

 

TECHNICAL SPECIFICATIONS

Scan area 

35 × 43 cm

Sensitivity (limit of detection) 

Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): < 2 dpm/mm2; 32P: 5-10-fold lower than 14C
488 nm excited fluorescence:  100 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence:  200 amol HEX, TMR, ROX, and 400 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence:  200 amol Cy5 end-labeled DNA primer in 0.4 mm thick, 12% polyacrylamide gel with TBE buffer. 

Pixel size 

1000, 500, 200, 100, 50, 25, and 10 µm, selectable.

Spatial resolution 

Autoradiography: 2 line pairs/mm for lines drawn with 14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mm 

Uniformity 

± 5% over entire scan area. 

Pixel accuracy 

± 0.15% 

Data format 

16-bit (65 536 levels), TIFF 

Linear dynamic range and linearity 

Five orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range. 

Red light source 

Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10 000 h Wavelength: 632.8 nm

Green light source 

Type: 20 mW solid-state doubled frequency SYAG laser  Estimated average lifetime: ~ 10 000 h Wavelength: 532 nm

Blue light source 

Type: 30 mW all blue lines Argon ion laser  Estimated average lifetime: ~ 5000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW) 

External interface 

10 Base-T Ethernet using TCP/IP protocol

Software 

Scan control software for Windows™ operating system. 

Power requirements 

115/230 V (switchable), 50-60 Hz, < 500 W 

Dimensions
(W × D × H) 

Instrument: 118 × 78 × 48 cm
Blue laser module: 30 × 78 × 48 cm 


 

In-lane sizing of PCR products. GeneScan -500 ROX size standard (yellow, Applied Biosystems), Cy3-labeled PCR products (green), Alfexpress Cy5 Sizer™ 50-500 (red, GE Healthcare) and fluorescein-labeled PCR products (blue) were resolved in a 12% polyacrylamide gel glass sandwich. (image from GE Healthcare)