3D Viewing and Analysis

Drosophila embryo stained with anti-a-tubulin (green) and anti-VASA (red) antibody. Projection of confocal z-series images shows the germ line cells (arrow, stained with anti-VASA) inside of the embryos. Cross-sectional view of the embryos along the dashed line clearly reveals the relative location of these cells in 3D. Images were taken and processed with Olympus FV1000 MPE microscope. 

                 Projected image                      Cross-sectional view of 3D image

The 3D viewing module in Olympus FV1000 program allows you to turn the 3D image in any direction and to simultaneouly visualize the cross-sectional views from x, y, and z axes.

Double immunofluorescence

In this figure, Eden Kleiman, using Leica SP2 confocal microscope, clearly demonstrates that PKCbetaII inhibitor CGP53353 inhibits Akt phosphorylation and subcellular redistribution in 3T3-L1 adipocytes.

Using an elegant plasma membrane sheet assay, Eden further confirms that CGP53353 blocks GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes.